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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7 results for: sds

Showing: 1 - 7 of 7 results

  1. Western Blot

    Type
    Protocol
    ...β-mercaptoethanol 4X protein loading buffer Precast SDS-PAGE gel SDS-PAGE running buffer Prestained protein ladder...the gel into the chamber of the SDS-PAGE gel tank. Pro-Tip Different SDS-PAGE gel tanks will have different...protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting. Protocols...protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting. Sharing speeds science...General Guidelines Acrylamide percentage varies in SDS-PAGE gels. Use lower percentage acrylamide when immunoblotting...2022 Workflow Timeline Day 1: Prepare lysates, run SDS-PAGE, transfer, block, incubate with primary antibody...protein concentration and prepare the lysate for SDS-PAGE Determine the protein concentration using a ...

  2. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...Update: June 14, 2023 Workflow Timeline Day 1: Run SDS-PAGE and stain gel Day 1 or later: Image analysis...1mm thick, 10-well, Invitrogen NP0321BOX 20X MOPS SDS running buffer, Invitrogen NP0001 4X NuPage sample...water. Gently invert to mix. Procedure Section 1: SDS-PAGE Add 5 µL of 4X sample buffer to each sample....

  5. Kit Free RNA Extraction

    Type
    Protocol
    ...Glycogen (Optional) Caution Make sure to read the SDS (Safety Data Sheet) for safety warnings and hazards...

  6. Weighing Reagents Protocol

    Type
    Protocol
    .... Read the reagent’s material safety data sheet (MSDS) to determine if this is best. Check that the balance...biohazardous. Check your lab’s material safety data sheets (MSDS) to understand how to properly dispose of reagents...
Showing: 1 - 7 of 7 results