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We narrowed to 286 results for: tac

Showing: 1 - 20 of 286 results

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a functional hybrid derived from the trp...

  6. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein...cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70 Intact Cells ... Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized cells  Cy5NTAc 43 34-62 YFP   ...   Lumio Red Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc Poly His  65 52-94 Plasma membrane protein, permeabilized...resulting in site-specific incorporation of the attached fluorophore. However, since tRNA suppression competes...cell membranes, thus enabling FRET measurements in intact cells. The Gradia Lab has deposited a gateway vector...permeabilized cells  Cy5NTAc 59 47-85 a R0 is the donor/acceptor (D/A) distance at which 50% FRET...

  7. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

  8. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...improve resolution, use a stacking gel on top of the resolving gel. A stacking gel typically has a different...protein’s secondary and tertiary structures remain intact and the protein is separated through a matrix by...of the proteins in a sample migrate through the stacking gel at the same pace and enter the resolving gel... causing proteins to migrate out of the gel and attach to the membrane.  In a wet transfer system the ...Figure 2: The western blot dry apparatus includes a stack of filter paper, a copper anode/iblot gel matrix...

  9. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...gills. It attaches there until they mature and changes sex from male to female. Then, it detaches from the...Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green algae (Soo et al., 2015...those linked to neurotransmitter activity and geotactic behavior (Biron et al., 2005).   References Biron...fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." Copeia 1983.3 (1983): 813-816. Gilbert...

  10. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...endogenous molecular target, meaning the dye will be attached to the target through binding of the molecular...encoded tag is required. These dyes can also be attached to an antibody to detect various targets, such...or glycans. Fluorophores can also be covalently attached to self-labeling proteins. Some common examples...covalently reacts with a small substrate ligand motif attached to a fluorophore. When paired with a protein of...higher specificity, increased versatility in probe attachment, and compatibility with far-red and near-infrared...Isothiocyanate-conjugated Antibody. Salari, M., Bitounis, D., Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, ...

  11. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ... know better. Networking is not about how many contacts you have; it’s about building genuine relationships...authenticity and not discomfort: Invite the new contact to connect on LinkedIn and always include a personal...profile link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters...postdoc association. It was 25 years since our last contact, but we were delighted to be reconnected.  Science... small community and you will come across your contacts over the years in many different ways. This leads...

  12. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...Research tackling questions in the fields of Cancer, Inflammation and Immunity, as well as various combinations...Researchers and Immunologists are coming together to tackle the challenge of curing cancer in cross-disciplinary.... to understand cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile a few...are preparing and carrying out this multi-pronged attack. Inflammation and tumorigenesis The conference...d like to share with the scientific community, contact [email protected] to find out more about our ...

  13. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...sequence. Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites...canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. The loxP sequence does not occur naturally...

  14. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ... your plasmids back to us. Our scientists will contact you if any issues arise during the QC process. ...collection that is greater than 75 plasmids, please contact us at [email protected]. These deposits are considered...strain in the “Growth Strain Other” field, and contact Addgene. We may require that you send your samples...in bacteria, select “yes” in the drop-down and contact Addgene. We can only accept plasmids that can be...to prepare and ship the samples to Addgene and contact your technology transfer office about the MTA. ...

  15. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...irreversibly with a small substrate ligand motif attached to a fluorophore (Figure 1b). Engineered ligases...phosphopantetheinyltransferase) – These enzymes catalyze the covalent attachment of a fluorophore ligand to a peptide tag (Figure...bioorthogonal chemical reactions (i.e., click chemistry) to attach a fluorophore at the specific site of incorporation... the speed and selectivity of the fluorophore attachment, the brightness of the resulting conjugate, and...for samples where washing is difficult, such as intact tissue. Some chemical labeling strategies such ...

  16. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...cofactor that in its active form is covalently attached to the active site of key metabolic enzymes including...Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...Photobiotin acetate consists of a biotin molecule attached to a photoreactive arylazide group. Under strong...methods. Scientists have commonly used this method to attach proteins or polypeptides to a solid substrate (... & Cronan, J. E. “Molecular Biology of Biotin Attachment to Proteins; Symposium: Nutrition, Biochemistry...

  17. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...professional internationalization faces several obstacles; for instance there are few resources to help ...University of Valencia): “It is very inspiring to be in contact with mentors who are at a professional level where...professional collaborations, setting up bases and contacts with Spanish laboratories to start working on ...needed to pursue them, increased their network of contacts important for their career goals, and identified.... Two independent scientists, who did not have contact with the program, evaluated survey results. Participants...

  18. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...transfection in: 15 cm dishes OR CF5 stacks Select how many dishes or stacks you plan to transfect: 1 to ...following μg of each plasmid needed per dish or stack. Calculations are based on using 28 μg of total ...) per 15 cm dish or 2500 μg of plasmids per CF5 stack. Total ug of each plasmid needed for the whole experiment...

  19. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...bolster and modulate our immune defenses to better attack cancer. Combing our knowledge with our bodies’ ...created by the cancer cells to shield themselves from attack. It is a region of incessant chemical activity,...that suppress APCs. T cells that would normally attack the cancer cells are also suppressed by chemical...microenvironment TGFβ, IL-10, can also help the immune system attack tumors. Attempts have been made to use whole cell...collaborative therapies must be explored. When attacking cancer, multiple immunotherapy targets can be ...

  20. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome to Montana...and I have an internal motto for scientists who contact us and want a guarantee that their experiments ...really, really hard. That so many scientists are tackling this type of genetic engineering is a testament...
Showing: 1 - 20 of 286 results