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Showing: 1 - 20 of 693 results
  1. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at...talked to people with industry experience, the more I heard that getting into industry early would only help...to postdocs in both industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic..., many believe that industry postdocs, having already been immersed in an industry environment, have a...biotech. In other words, industry postdocs already have the prerequisite “industry experience” that I’ve ...as a requirement on some industry job postings. Also, if you work in industry for any length of time, ...be higher in an industry setting. Scientific community and mentorship: As an industry postdoc, you might...
  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this...experience not long ago. I just recently started working in the pharmaceuticals industry after earning my Master...jobs, I interviewed with just the PI of the lab and for one position, I just had a phone interview. I ...you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready...between applying for an academic position versus an industry position is the on-site interviewing process. ...groups without having a formal interview process. Industry interviews, however, were more structured. I met...is what I’ve found helpful for my interviews in industry. Prepare stories to tell in your interview The...
  3. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed gRNAs and ...
  4. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...had quite the up-tick in views over the month of August - at over 56,000 views, we’ve grown 12% over last...surprising given how many scientists go on vacation in August (for another part of my job, I travel around the...posts as each one has something great to offer, I just need to get back to emailing (hopefully non-vacationing...non-vacationing) scientists. You can find all of our August posts in chronological order here: Site Directed...
  5. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ... the second in a series of posts on bringing sustainable practices in the lab. The first post focused ...learn about some creative approaches to lab sustainability, and find ways to further reduce our consumption...consumption beyond recycling.     Choosing more sustainable products Recycling is great for rerouting waste... grant agencies), we can shift toward a more sustainable one. The math on this is pretty simple: wash ...grades of plastic lumber and distribute it to industrial and residential markets. Rethinking our approach...
  6. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...more lab sustainability tips, check out our other articles in this series: Bringing Sustainable Practices... specialized companies can recycle plastic-- you just have to find them (or keep reading). Corning takes...Encourage your workplace to become a leader in sustainability. Sign up for blog posts and updates from ...individuals, companies, and universities to be sustainability leaders. Take initiative to bring ideas to ... A lot of places have small or non-existent sustainability offices, so it’s up to all of us to work on...Practices to the Lab: Innovations Bringing Sustainable Practices to the Lab: Easy Action Items References...
  7. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...is a new software tool used to design gRNAs for custom CRISPR screens targeting a (long or short) list...Glance!  CRISPR knockout screens: Full genome vs. custom screens Full genome CRISPR knockout screens have... being involved in the studied phenotype. For robustness of a screen, each gene is not targeted by only...hypothesis driven research question. Examples of custom CRISPR screens Differentially expressed genes  ...massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...microbe or a cytokine is added to a cell). With a custom CRISPR screen and the appropriate phenotypic readout...
  8. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ... and increase your lab sustainability. For my other posts on lab sustainability, check out my Recycling...This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy...unimportant. With so many big contributors to the sustainability problem, how can one little change make a difference...quick things above, try joining or starting a sustainability project. This could be: Setting up a recycling...Learn about other creative approaches for lab sustainability Resources on Addgene.org Sign up for our ...
  9. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a...of our core values here is to provide unmatched customer support, as this is vital to ensuring that our...technical, or quality control issues. Improving customer support with self service We strive to make our... at the moment you’re looking for it, or you may just prefer to troubleshoot yourself without contacting...waiting for an answer. Luckily, there’s a trend in customer support to address this exact problem, and it’... find the answer that you’re looking for, or you just have a general question or concern about the answers... customer support...
  10. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    Published
    Oct. 5, 2017, 1:13 p.m.
    ...What's a lab group in industry? The collaborative nature of working in industry and how delegating can...Crystal’s exciting career path, to gain insight into industry research, and to get advice on how to succeed ...importance of thinking about the big picture and not just the next experiment. Chapter 6: Advice to scientists...scientists who are planning on working in industry. The importance of recognizing and leveraging your unique...adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings. Mobility between...
  11. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...from.   Acoustic reporter genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene clusters that ...optimized for expression in E. coli: acoustic gene 1 (arg1) and acoustic gene 2 (arg2). The key difference...Bourdeau et al calls this method acoustic collapse.  Importantly, acoustic collapse doesn’t affect bacterial...for serial acoustic collapse to aid in the development of ARG constructs with new acoustic phenotypes....but each of these reporters has its drawbacks. Acoustic reporter genes (ARGs) overcome these limitations...possible if bacteria are sequentially exposed to acoustic pulses with high enough amplitudes to first collapse...viability and with 20 hours of growth following acoustic collapse E. coli re-express arg1 gas vesicles ...
  12. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used...members of our lab - the Shapiro Lab - developed Acoustic Reporter Genes (ARGs), which are sets of genes...inside the body. So we screened gas vesicles gene clusters from a diverse set of gas vesicle-expressing species...39006 produced the highest levels of nonlinear acoustic contrast in E. coli. We deleted an unannotated...the first-generation bARGs, poor expression and acoustic properties of these gas vesicles made non-destructive...expression, a smaller genetic footprint, and nonlinear acoustic scattering. The best candidate for this was the...the Anabaena flos-aquae gas vesicle gene cluster because of its small size and its ability to produce ...
  13. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...available to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists...available to industry scientists To make plasmid sharing more open for academic and industry scientists,...available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’s COVID-19 resources... to Addgene’s new approach of working with the industry-based scientific community. Conversations with...Academy of Medical Sciences University of Texas at Austin University of California, San Francisco  University...to the scientific community - both academic and industry scientists. This partnership complemented each...we have other plasmid collections available to industry? Over the past 8 years, several organizations ...
  14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ... et al., Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive imaging of microbes...Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In recent years,...solution, Mikhail Shapiro’s lab has developed four acoustic reporter gene (ARG) plasmids to better monitor...the ARG plasmids at Addgene. For more details on acoustic reporters, check out this blog post. Bourdeau ...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...
  15. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...Having spent that long in customer service, I've daydreamed about the ideal customer service interaction -...to any biology-related customer support interaction. Before contacting customer service In an age of Tweeting...you haven’t received a response, just keep in mind that our customer support scientists wear many hats...answered hundreds of phone calls and thousands of customer service emails in my six years as a senior scientist... - one that gives our customers the most utility in the least amount of time. Though I now spend far less...my years of accumulated wisdom so that you, the customer, can get the most out of your email or phone call... that everything I could have wanted to know was just buried beneath someone's lunch. Many times, I’m ...
  16. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...support through the customer’s perspective is good. If you’ve never participated in a customer support conversation...the types of questions customers ask, and there are also differences in customers’ personalities and their... roles in industry. What to look forward to in a technical support role Talking to customers everyday,...because there are just so many things which can go wrong in a lengthy protocol. And customers may tell all ...be enhanced on the job. When dealing with customers, you must be polite, friendly, patient, attentive, ...lab to industry without being too commercial or “sales-y”. The work includes reviewing customer data sets... product issue in the lab. The issue was with a custom-made reagent to manipulate cells (manufactured ...
  17. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...little different. Industry and academic jobs do exist, but working in industry just wasn't for me, and...applied for a role in Industry. I wanted to get "a real job" and roles in Industry do tend to pay better... (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however...Research in Australia I then travelled abroad to work at the University of Queensland in Australia, this was...months in Australia or another country of the Commonwealth. Another reason why I chose Australia was because...cancer. This was particularly interesting as I had just completed my thesis on the study of e2Fb, which ...roles in the field require experience. Working in industry: Microbiology On returning from Ghana at the beginning...
  18. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...transferred to an industry position. If you cannot get an Industry H-1B, remember that industry career paths...Subject Industry H1B Academic H1B National Interest Waiver EB-1A EB-1B Cap subject industry H-1B There...endeavor must have both substantial merit and national importance; (2) The foreign national must be well...review. The peer review request must be directed to you specifically. You must also complete the peer review...available to you including careers in academia, industry, non-profit, and government sectors.  Within these... Admission of nonimmigrants). The foreign worker must possess at least a bachelor's degree or its equivalent...-international focus. Candidates in need of an industry H-1B need to be aware of the April 1 deadline ...
  19. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...things are more frustrating than being on a leaderless team. Sometimes the leader just has to be the person...scores. That player doesn’t trust his teammates; the teammates don’t trust him. This team is not going...responsible for a group of specific processes, they must work in sync to get tasks done. At Addgene, the ...performing quality control, and providing technical customer service. Team members are highly trained, and ... a very broad term since a project goal could be just about anything. Is the goal of your project to launch...necessarily want a lot of redundant skills - you just want the necessary skills. You might find that your...of people to provide that base. Sometimes there’s just too much work If you are one of those graduate students...
  20. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...employees overtime, salaries must be above this level, and certain job duty tests must be met. In the Department... is over and instead we must make real changes to make the system sustainable. For years, there have been...that the salary level should now be automatically adjusted every 3 years. In May 2016, Secretary of Labor... that while they recommend NRSA levels, postdocs must be at least at the FLSA minimum of $23,660. Any ...institutions, we can say with relative certainty that just over 40% of current postdocs will have their salaries...writing responses to requests for the legal justification of this have not been received. Future actions... funding climate combined with the lack of a sustainable research enterprise, there is no easy way for...
Showing: 1 - 20 of 693 results