We narrowed to 803 results for: Cre

...added flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC). To make...have used PCR-based screening to detect mutants, but this method limits screening to a few hundred worms... less than eight hours worth of hands-on time. Creating a modular, selection-based gene tagging approach...rare events difficult. Dickinson et al. sought to create a new and efficient selection-based CRISPR/Cas9...construct will serve as the repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The...knock-ins. These candidate animals can then be further screened based on their Roller phenotype. If SEC is inserted...separates the targeted gene from its promoter, creating a loss-of-function allele as well as a promoter-FP...

...modular expression systems exist (eg. LexA/LexAop and Cre/Lox systems) in case you want to express one gene...Drosophila geneticists use the Gal4/UAS system. This incredibly useful, yet simple system allows you to systematically...simpler, this modular system saves you from having to create a new fly line every time you want to express YFG...

... Resources on the Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Interview...artificial intelligence (including a team that created a novel form of AI that can learn new tasks and...

...Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome Engineering Posts...

...FLP)-OCaMP-WPRE pAAV Ef1a pAAV-hSyn-FLEX(cre)-OCaMP-WPRE pAAV hSyn pAAV-CMV-OCaMP-WPRE.... These industrial fiber lasers are becoming increasingly common due to their low cost and high power,...single AP. B) Averaged fluorescence responses to increasing numbers of evoked APs in neurons expressing OCaMP...

...Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with GFP Resources ...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three.... The Problem:  There is no common method for creating optically controlled kinases. The Solution: In...et al, the Lin lab present a generic design for creating photoswitchable kinases and use MEK1 as a model...often hard to decipher because it’s difficult to create short pulses of kinase activity. Zhou et al studied...feedback on endogenous MEK1/2 phosphorylation by creating a short pulse of psRaf1 activity. They did this...just 5 minutes post-Raf1 inactivation, and then decreases. Treatment of the cells with a PP1/PP2A inhibitor...

...delete the functional portion of the Nfix gene using Cre recombinase and to generate Nfix-negative neural ...burden of immune symptoms on CDG patients and to increase their quality of life by reducing their risks ...ligand binding sites in T4 lysozyme by deficiency-creating substitutions." Journal of molecular biology 277.2...

... expression, and compatible with constitutive or Cre-dependent expression. These sensors have also been...binding sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity), and is a ...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...illumination with violet light and (2) exposure to increased intracellular calcium. It has been applied to ...

...expression levels of certain genes. A popular method uses Cre recombinase on transgenic flox animals to introduce...light or noise should be avoided as this will also increase stress levels and affect behavior. A fact to consider... also to minimize the damage of tissue due to increased pressure of the added liquid. The volume should...editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency can be observed after...

...modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA or Cas9 mRNA and sgRNA...for its small size. They were able to increase activity and decrease the gRNA size by intensively remodelling... engineered guide RNAs Yong-Sam Kim’s lab also created a miniature yet active Cas system starting with...