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We narrowed to 790 results for: Cre

Showing: 91 - 100 of 790 results

  1. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...modular expression systems exist (eg. LexA/LexAop and Cre/Lox systems) in case you want to express one gene...Drosophila geneticists use the Gal4/UAS system. This incredibly useful, yet simple system allows you to systematically...simpler, this modular system saves you from having to create a new fly line every time you want to express YFG...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA or Cas9 mRNA and sgRNA...for its small size. They were able to increase activity and decrease the gRNA size by intensively remodelling... engineered guide RNAs Yong-Sam Kim’s lab also created a miniature yet active Cas system starting with...

  4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...dual virus and the experimental approach. (B) CR-Cre mice demonstrate pain response due to blue light ...microcircuits. “In the longer term, this screening platform may help us to create high-performance sensors to enable...mechanical allodynia, a condition where a light touch creates a painful sensation. The dorsal horn of the spinal...virus expression in CR neurons of the spinal cord. Created with BioRender.com. Image from Haoyi Qiu.   ...indicators. Tian developed a video-based high-throughput screen for directed evolution of biosensors based on their... to engineer many types of biosensors.   This screening method allowed Tian to optimize a red‑shifted ... the improved voltage indicator coming from the screen to understand mouse cortical and hippocampal neural...

  5. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...expression levels of certain genes. A popular method uses Cre recombinase on transgenic flox animals to introduce...light or noise should be avoided as this will also increase stress levels and affect behavior. A fact to consider... also to minimize the damage of tissue due to increased pressure of the added liquid. The volume should...editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency can be observed after...

  6. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... expression, and compatible with constitutive or Cre-dependent expression. These sensors have also been...binding sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity), and is a ...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...illumination with violet light and (2) exposure to increased intracellular calcium. It has been applied to ...

  7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...delete the functional portion of the Nfix gene using Cre recombinase and to generate Nfix-negative neural ...burden of immune symptoms on CDG patients and to increase their quality of life by reducing their risks ...ligand binding sites in T4 lysozyme by deficiency-creating substitutions." Journal of molecular biology 277.2...

  8. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with GFP Resources ...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three.... The Problem:  There is no common method for creating optically controlled kinases. The Solution: In...et al, the Lin lab present a generic design for creating photoswitchable kinases and use MEK1 as a model...often hard to decipher because it’s difficult to create short pulses of kinase activity. Zhou et al studied...feedback on endogenous MEK1/2 phosphorylation by creating a short pulse of psRaf1 activity. They did this...just 5 minutes post-Raf1 inactivation, and then decreases. Treatment of the cells with a PP1/PP2A inhibitor...

  9. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ..., lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression, insect expression...and Organization where your plasmids were first created. From there, we’ll use the information in the ...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...light-dependent Gal4 transcriptional activation and Cre recombination. Find PhoCl plasmids at Addgene. Zhang... for easy removal of the resistance cassette via Cre recombination. Each knockout includes unique barcodes...natural ancestors), and screened through libraries of new RFP variants for increased fluorescence, brightness...polycistronic cassettes and discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal... did not lead to the observed reprogramming discrepancies. The polycistronic piggyBac transposon vectors... protein PhoCl expands optogenetics toolbox Increasingly popular Optogenetics tools use light to alter...PMID: 28241009 CRISPR coselection with ouabain increases editing efficiency Blue flame depositor Yannick...
Showing: 91 - 100 of 790 results