...Scientists are known for asking "What? and "How?" , but they are not always known for asking "Should we?". Scientists...discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal cord ...primary Addgene contact for many years, I've been looking forward to seeing how the sharing of his OSKM factors...clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic due to ...techniques are neither fast nor inexpensive.  After talking to numerous poster presenters, it was gratifying... Eric J. Perkins...

...affinity and slower kinetics iGluSnFR3 v857: decreased affinity and faster kinetics which are illustrated... it is selectively activated by glutamate, and tweaking it to improve its responsiveness, they showed ...Improve sensor’s photophysical properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum...v82) and lime green (v857) variants differing in kinetics, affinity, and ∆F/F0. Introduce the two variants..., GPI, and SGZ, to improve neuronal membrane trafficking and localization. Improved photophysical properties...al…, Supplementary Figure 2.   Improved trafficking and localization through different C-terminal...“Glutamate indicators with improved activation kinetics and localiation for imaging synaptic transmission...

...last for several days.” Working with CRISPR Recently Doench has been working on developing CRISPR pooled...not, well, your grade is lower.' ... When you are waking up on a Saturday at like 7 am to go check your ...” Doench has spent most of his research career working on miRNAs and RNAi. He first heard about RNAi at...technology development alongside it.” Doench starting working with David Root in the then RNAi Platform, which...investigate the human genome. Doench says he enjoys working on technology development under the Platform model... but work in true collaboration with scientists asking biological questions. Doench loves that he still... “very much in line with the overall mission of making science easier and more transparent. That’s largely...

...consider when seeking your dream lab. The principal investigator's point of view If you are looking to join...decision. A science trainee will spend 4-7 years working for one person and with a group who all strive ...join a lab, you may be wondering “What is this PI looking for in a new researcher?,” “How do I convince the...typically go through a rotation, which reveals their working habits, personality, and gives them the opportunity...style to spur you to succeed or you might enjoy working closely with others to accomplish your goals. You... You must understand your own style before making this choice.  How big is the lab? While larger labs ... Dr. George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists...

...by your library. After blocking for 1 h at room temperature with gentle shaking, gently wick off excess...display little to no homology to anything else, making it difficult to quickly identify possible gene ... overexpression in E. coli, such as pET30a. If working with DNA that is refractory to enzymatic manipulation...the T7 polymerase and a few proteases knocked out making them particularly useful for protein production...harvested dose of cells (preferably in log phase) in blocking solution (around 108 CFU/mL is a good place to...start) and add your membranes to the solution, shaking gently for 1 h at room temperature. This will allow...onto nitrocellulose and carry out the steps from blocking the membrane to probing with cells in order to...

...improve resolution, use a stacking gel on top of the resolving gel. A stacking gel typically has a different...buffer, blocking the membrane  from binding to the antibodies during the stain. The ideal blocking buffer...frequently include suggestions for working concentrations, blocking buffers, and incubation times. If ...interested in making your own knockout line for validation, please view this tutorial from Stuart Orkin's and Daniel...of the proteins in a sample migrate through the stacking gel at the same pace and enter the resolving gel...

...protein-peptide locking, Mark Howarth’s lab has developed a new tool for peptide-peptide locking. The new technology...light-activated receptor tyrosine kinases Receptor tyrosine kinases (RTKs) are a large class of cell-surface...informational videos, download protocols, and more! Looking for background information on CRISPR technology...Easy-to-use tools to monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed...described as “knocksideways” (a British idiom for “taking by surprise”) by Margaret Robinson’s lab, and more...mitochondrial membrane. Sequestration is accomplished by knocking down your endogenous protein of interest and co-expressing... blue and green light-excitable and has faster kinetics than those of other channelrhodopsins. CnChR1,...

... from Leah Bury of Microscopic Art. I started thinking about this problem when I recently attended the...the transcription of a gene a megabase away, or looking at the histone methylation status at a double-strand...double-strand break that needs repairing. They may be looking at chromatin at the level of nucleosomes, loops... other areas of biological research. If you’re looking for resources that will help you study how chromatin...with Addgene, but you’ll find many great tools by clicking on the links below and visiting their associated...Sharon Dent Archa Fox Elaine Fuchs Kai Ge Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer Alexander... listed here. If you don’t see the gene you’re looking for on this list, please consider searching directly...

...information, making it available to everyone, that creates more breakthrough and innovation. With any kind of open...'s operations. We’re looking for those “what could go wrong scenarios” and making sure we have adequate...than just picking up the phone and having a discussion. By only relying on email and never picking up the... more about Addgene and heard that Addgene was looking for new board members. Paratore: Kristen Darby,...goals were and so I decided to join. I’ve been working with Addgene since 2014 so I’ve been able to watch...

...organizations with similar ideas who were also thinking about reproducibility and shared similar desires...Fortunately, the eLife ambassadors group was already thinking about ways to tackle irreproducibility. Benjamin...organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility and thanks... post-workshop surveys, recording results, and tracking material downloads. This was particularly helpful...community. Lessons learned Instead of continuously talking about a problem and potential solutions, take actionable..., but how do you get them to connect? Through networking of course! Thanks to individuals in the group...right time. Be inclusive and seek solutions by working with communities, not for them - Another strength...