We narrowed to 623 results for: SCR

...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert amplified...2018) CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science 360...

...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...very relieved not to be going through the urgent scramble of their peer institutions. The issues facing ..., and it is becoming ever clearer that outside scrutiny of science and the academic system is increasing...

...methyltransferase — that can drive lasting transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration...versions using dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen...Weissman, J. S. (2021). Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell...

...Easi-CRISPR in vitro transcribes RNA encoding your repair template, then uses reverse transcriptase to generate..., and ultraviolet light. In this post, we will describe the mechanism of homology directed repair, one...

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...library that’s ready to be tested in a functional screen of your liking. This method is similar to site-directed... screen...

...same fluorescent markers, Brainbow 3.1 and 3.2 (described in the same publication) are unique because by...cell type. The Confetti mouse was first used to describe the self-renewal of cells in the intestinal crypt...previous Brainbow technologies were useful for discriminating neurons within only one field of view. Tetbow...

...strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to reduce transcriptional readthrough...worked too. Rhamnose induction relies on the transcriptional activator rhaS. To allow manipulation of bacteria...

...site-specific incorporation of phosphoserine (as described below) provides researchers with a valuable tool...context-specific effects of UAG suppression, as described (6). To determine the stoichiometry of phospho-incorporated...phospho:non-phospho species on PhosTag gels as described above. Reagents and protocols Sep-OTSλ plasmid...

...pathological condensates, including oncogenic transcription, proliferation of leukemia cells, and assembly...easily visible chromoproteins in a cell-free transcription-translation system (Figure 6). This approach...CRISPRkit uses a cell-free system to carry out transcription and translation of chromoprotein and gRNA plasmids...

...applications, including transgenesis, gene-trap screens, and gene editing. How does the piggyBac™ system...effects, such as with signaling molecules or transcription factors, or when the gene is constitutively-..., since there is no selection, you will need to screen somewhere between one hundred and one thousand ...

...Antibody-independent methods may include analysis of transcriptomic or proteomic data from several different tissues... feasible and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want...tissues. You know that there is a wealth of transcriptomic data from this sample type, so you use your...

...brightness of individual fluorophores. Table 1 describes the brightness of a few commonly used fluorophores...brightness levels on your smartphone or computer screen), and you should be able to properly label many...panel! Designing a Panel In this example, I will describe a basic panel (Table 2, Figure 5) for a hypothetical...

... drug discovery by big pharma (i.e. HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular...are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET...it all together The FRET donors and acceptors described above can be paired together to measure a wide...

... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding...can even be used to detect a single copy of a transcript. But this sensitivity can sometimes cause as ...

...Noninvasive assessment of gut function using transcriptional recording sentinel cells! 🎵 On the 11th day...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...

...various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR...

...populate your “PubMed filter list” to the left of your screen. That’s it! Next time you log-in to your PubMed...Scientific Review Article Tips for Improving Your Next Manuscript Resources on Addgene.org Learn How to Order...

...used to increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target...cell population. The resulting mutant cells are screened for phenotypes of interest. Limitations of CRISPR...

... Just click on the search bar at the top of the screen and type in what you’re looking for. After you ...know about new articles on the Addgene blog by subscribing to instant alerts below.  ...

...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...

...individually isolated and expanded. The clonal cells are screened to identify hybridoma lines, each producing a ...antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET...

...Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to...to an Mxi1 repressor domain repressed transcription of HSP90 an essential C. albicans gene. Read the ...

...plasmid page. Check out the purple arrow in the screenshot below to see what I mean. Additional features...increase the length of the list (purple oval in the screenshot below). You can also use the "Search Table" box...

...rescue organization where I foster dogs and help screen dog adoption applications. My most recent foster...about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog Resources on addgene.org:...

...including beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA for use as a negative control, and empty...rapidly in 2011, from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). ...

...supporting the tools in our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub... to learn more about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi,...

...communication between all members of a team. As described here in a 2012 Intuit blog post, a study coming...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

...mutations The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and...targeted therapeutic interventions for cancer. This screening platform is an incredible boost to the cancer ...

...updated! Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene...

...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...