We narrowed to 662 results for: Clu

...– it makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and...which have fragments of the AdV genome in them, including the E1 region). The space in the viral particle...

...Biology (gDNA → mRNA → protein): their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its...will simply express from whatever promoters were included in the vector (or are nearby in the host genome...

...to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have been reported...al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated purification of a specific genomic...

...mammalian production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese ...specifically. Possible applications for validation include western blot, immunohistochemistry, and immunocytochemistry...

...the primer sequences. Multiple plasmids can be included in a single PCR, for example for subcloning genes...vector. The new codon and homologous region can be included at the 5’ end of either primer. Subcloning - To...

...and its RNA directed binding site. These methods include the fusion of an activation domain such as VP16... or tracrRNA sequences can also be modified to include RNA domains that recruit additional transcription...

...accompanying cartoon, they have a multitude of uses including (but not limited to) purification, detection, ...combinations, though frequently those combinations still include at least one element from the original TAP tag....

... CRISPR complex much easier in various cells, including primary mouse cells. We believe that this system...sight in that Addgene takes care of everything including MTA and shipping. Addgene realizes the One-Stop...

...method can be used for an array of applications including clinical diagnostics (e.g. pathogen or virus detection...Cas12a is initiated. Surrounding trans-ssDNA, including the ssDNA-FQ reporter are subsequently degraded...

... works on our site. For Addgenies, development includes everything from tracking plasmids from the moment...then go back to this area later and tidy it up. Conclusion While none of us are life science majors, we ...

...to the total number of viral capsids (which can include empty capsids that are devoid of a viral genome... et al., 2010). In addition to the AAV RSM, we include a second AAV reference sample of known titer, which...

... in the linings of certain areas of the body, including the lung, the heart, and the abdomen. Long story...primary conventional treatments for mesothelioma include surgery to remove cancerous masses (tumor resection...

...target the ubiquitin E3 ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein...mScarlet-I3 that were codon-optimized for C. elegans and included artificial introns to improve expression. When...

...show your enthusiasm for getting the position and include a brief summary of why you are a good applicant...taught for five years at [name of the university]” Include keywords from the job description to help the hiring...

...antibiotic resistance develop? Microbes (bacteria included) are very skilled at the delicate art of survival... contributing to the development of resistance include (Figure 1): Over-prescription of antibiotics (...

...citing research from outside of their disciplines. Included in the article is an interactive chart where you...is of interest. In his research, Scott Spicer concludes that “… the emergence of the video abstract genre...

...number of potentially hostile posters (this advice included leaving out the term “implicit bias” which is ...rewriting or coming up with answers, I was able to include links to these posts and eBooks. I also have ...

...burden from getting overwhelming. These mechanisms include efforts like flexible staffing on case teams, where...offices, classes at business schools, and consulting clubs are especially helpful since they both provide up-to-date...

...subtle and may include reduced saturation density, decreased rates of growth, or clumping. To ensure the...

...dominant-negative and/or toxic. A potential solution is to include an in-frame degron, or degradation sequence, upstream...expressing a truncated protein. This can be avoided by excluding a stop codon from donor A in any of the reading...

...everyone who visits your poster to agree with your conclusions. That conversation you’re having--that conflict...of the conflict. It can be easy to leap to the conclusion that a conflict is personal (”Person A and Person...

...insoluble inclusion bodies. While it’s possible to re-solubilize and re-fold scFvs from inclusion bodies,...

...Build: Jennifer created a mobile camera area that included iPad and her laptop raised on an ironing board... the camera, to control some basic functions, including starting and stopping recording).  Test: It was...

...easy to follow seminar See item 2. Attendees will include grad students (that are solicited for feedback)...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...

...ideal model organism to study an array of topics including development, genetics, and the nervous system.... an array of biological systems and processes including the nervous system, development, cell signaling...

...addition to the XFP in the construct, dBrainbow includes small epitopes fused to each XFP. Secondary antibodies...post from the researchers who developed Tetbow. Conclusion Stochastic multicolor cell labeling systems allow...

...attached to the active site of key metabolic enzymes including biotin carboxylase and decarboxylases. These enzymes...oligonucleotides can be used in a multitude of applications including but not limited to in situ hybridization, blotting...

...if some of your work does relate, make sure to include that material, but don’t force it. You can always... Having answer slides ready after your formal conclusion slide will also help with your confidence. It...

...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications,...pSer-incorporated proteins on a single plasmid. This includes 4 copies of a tRNA that specifically recognizes...

... search suggests there are hundreds. This only includes general lab strains designed for subcloning or...or protein expression. If you were to include customized strains, the number is probably in the thousands...

...which uses light to regulate transcription, also includes light-regulated histone methyltransferases and...has deposited mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300...

...Cre recombinase contained within the knock-in. Including Cre-mediated marker excision, it takes approximately...that recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for...

...in yeast, plant, insect, and mammalian cells, including human cells. piggyBac is biased to integrate at...all been used to generate transgenic animals, including zebrafish, mice, rats, and rabbits (Sandoval-Villegas...

... still valuable for anyone in the lab though, including undergrads. I found a lot of career resources ...in microbes until the end of my second year! Conclusion and other pieces of advice Give yourself permission...

... utilizing chemogenetics (Designer Receptors Exclusively Activated By Designer Drugs, DREADDs) and optogenetics...examining the use of DREADDs (Designer Receptors Exclusively Activated By Designer Drugs) to determine the...

... infect both dividing and non-dividing cells, including stem cells. These properties make lentiviral vectors..., a separate genus of the Retroviridae family including human immunodeficiency virus type-1 (HIV-1), can...

...stop and plan it out. Some questions to answer include: What is the goal of the experiment? What are...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...

... detector and all light from above or below is excluded. Parallelized Confocal Microscopy (Spinning Disk... type of sample as they are most efficient at excluding out of focus light. However, they also continually...

...information related to your plasmid. It is optional to include the total size of the insert and the GenBank ID...submission, please email us at [email protected]. Include your deposit number and which data needs to be ...

... Paramyxoviridae family of viruses, which also includes measles and mumps. Sendai transduces a wide range...the field of iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC...