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Showing: 361 - 400 of 673 results

  1. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...

  2. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...for material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that...with the UBMTA result in quick approvals, so your plasmid requests can get to your lab faster. For Everybody...reference pages. So go ahead and use Addgene plasmids in your experiments. You know that they’re properly...

  3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...

  4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...transcription from the accessory plasmid (AP) encoding phage gene III. The accessory plasmid library contained all...PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...

  5. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...generation of mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found ...initiative to curate our collection of cancer-related plasmids such that each is linked to a known major pathway...pathway. Now, using our new Cancer Research Plasmids and Resources page, investigators may select a pathway...investigator to a list of all available Addgene plasmids he or she can use to study it (as seen in the ...Visit our Cancer Collection Pages Deposit Your Plasmids for Studying Cancer Browse Our Other Collections...

  6. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...: 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et ...4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, ... 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional Resources...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein ...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene.... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

  10. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ... great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow...antibodies can be produced through a single or dual-plasmid transfection, depending on whether the heavy and...and light chain genes are contained to one plasmid or if they’ve been separated onto two. You’ll first...first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to collect ...on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies...

  11. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...antibiotic selectable markers by the destination plasmid. Antibiotic selection allowed for high throughput...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...'s work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...

  12. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ... Tips for Writing a Good Cover Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates...Kinome Libraries Available: Pooled and Individual Plasmid Formats Better Dyeing Through Chemistry & Small...Addgene supporters who spoke with me and deposited plasmids on this trip. Here’s to hoping that October is...

  13. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...exact Cas9 plasmid used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and...find a link to search Addgene's collection for plasmids containing that enzyme, and embedded in the References...lists on every page you can find direct links to plasmids from the article if they're available through ...

  14. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...mammalian cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there...dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct for this variation,...Goedhart et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  15. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic microbes...granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones (the Cpn...pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a ...biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment Read our...

  16. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...that Addgene provides is an open repository of plasmids and other necessary information that’s required...the collection, storage, and distribution of the plasmids and their ability to identify and fulfill a need... helping the scientific community manage those plasmids so scientists aren’t bogged down with distributing...continuing to look at providing other things besides plasmids to the scientific community through Addgene’s ...

  17. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...received the first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that ...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...pool and extract plasmid DNA directly from the colonies. Transform the resultant plasmid DNA into E. coli...DNA bases.  Ligate and transform your resultant plasmid libraries into chemically competent E. coli and... you’re using a pET vector) and antibiotic for plasmid selection. Remove air bubbles between the membrane...they expressed well, the proteins encoded by the plasmids in the library will remain attached to the membrane...of Expression Vectors Deposit Your Microbiology Plasmids ...

  19. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...interest endogenously and transfect them with a plasmid encoding your protein of interest. Then you should...target protein in a Western blot. You acquire a plasmid encoding your protein of interest, known as Gene... In parallel, you transfect cells with another plasmid not encoding this protein, which we will call Negative...band from the cells transfected with the other plasmid. Your antibody works! Multiple (Independent) Antibodies...Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ...Designing a recombinant validation experiment? Find plasmids here!  References Baker M (2015) Reproducibility...

  20. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting...the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in ..., the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected...provided (in the form of an oligonucleotide or plasmid), precise changes can be made in the genome via...mice carrying the intended mutation. With a donor plasmid, the outcome is less predictable. In our best case...

  21. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ... from cleaning out the freezers to writing new plasmid protocols for our website. Free lunch is provided... labs, and people work hard at Addgene to make plasmid sharing as easy as possible. However, I think it...keeps us smiling even when we’re packing the 400th plasmid that day.   As we head into Thanksgiving weekend...

  22. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... that is something to recognize! Distributing plasmids, viral service, and more For the first time ...to worry about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% ...distribution to ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our ...Addgene Resources on the Addgene website Find plasmids for your research  Learn about our viral service...

  23. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ... a reporter and a nanobody that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...

  24. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...for expression of a gene carried on the transfer plasmid or quantifying the number of viral copies that ...beneficial as they ensure that the correct transfer plasmid was used in the transfection; if preparing several... that comes from amplification of the original plasmid which can over-inflate results. A packing vector...antibiotic resistance genes carried on transfer plasmids. Target cells are transduced with serial dilutions...

  25. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...Table 1). The four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME...  The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations...first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral Fellow...

  26. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...associated publication for the specifics of your chosen plasmid. Types of biosensors When choosing a biosensor...

  27. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...scFV antibody, which is expressed from a separate plasmid. This system amplifies the intensity of the fluorescent...basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it’s clear that the...Fluorescent Protein Guide Page Find Luciferase Plasmids ...

  28. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...look clean and easy to use.  Easily search for plasmids (and more!) The main goal for the homepage was...functionality. We wanted users to be able to find plasmids and viral vector tools more easily. In the redesign...hope every time you come to Addgene to find the plasmid or viral vector for your next experiment, that ...

  29. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...Addgene.org Find plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent...acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau...

  30. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ....1420024112. Epub 2015 Feb 23. Pubmed. Find the plasmids from this publication at Addgene. Dynamic imaging...10.1016/j.cell.2013.12.001. Pubmed. Find the plasmids from this publication at Addgene. A protein-....2014.09.039. Epub 2014 Oct 9. Pubmed. Find the plasmids from this publication at Addgene. ...

  31. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ... smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single infection system... thousands of targets at once. That’s what our plasmids allow.” Wang’s library is designed to target a...

  32. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...trying.  When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and...addgene.org! Fun fact! Did you know you can search for plasmids from a publication by pasting the paper's title...

  34. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Want to get your hands on SpRY? Find a mammalian plasmid here and edit on! References and Resources References..., 6175 (2023). DOI:10.1038/s41467-023-41829-y (Plasmids available here) Walton, R. T., et al., Unconstrained...368,290-296(2020). DOI:10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered... on Addgene.org Addgene’s CRISPR Guide CRISPR Plasmids and Resources Resources on the Addgene blog ...

  35. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

  36. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ... Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander...Experiment Deposit Your Plasmids with Addgene ...

  37. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...and writing of two blog posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications...

  38. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...topic or lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational...Addgene.org Check Our Our Protocol Pages Browse our Plasmid Reference Pages ...

  39. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...

  40. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate...
Showing: 361 - 400 of 673 results