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TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....You’ll first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to ...Barnett, Jai A. Pathak, Christopher J. Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation...
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CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing while reducing...from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even temperature change... all of the droplets, but you can go as low as 1C/s. Increasing the elongation time to 2 minutes and the... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m.... the genome to introduce the genetic modification(s) contained within it by selecting for Zeocin resistance...19416369. Kulakauskas, Saulius, Arvydas Lubys, and S. Dusko Ehrlich. "DNA restriction-modification systems... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....transgenic plants in the process. References 1. Emami, S., Yee, M. and Dinneny, J. R. (2013) ‘A robust family...24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method of identifying... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....Zebrafish (Danio rerio) have been used since the 1930’s in a range of biological studies, including investigations...data in useful ways. Zebrafish mutants In the 1990’s, two revolutionary large-scale random mutagenesis ... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m.... adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other Ly6 ... these two references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive gene... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....Vanderperre S, Paul R, Björklund M, Ahr D, Ahmed AW, Spinelli L, Brun C, Basler K, Merabet S (2018) Generation... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m.... to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination...doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of Lentiviral... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m.... Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome... this paper at Addgene. 3. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m.... Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros...Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318-22.... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....transferable. Use your experience and your passion(s) to guide you in building a grant career. Do you have... client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor, find... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m.... menschlichen und tierischen Praparaten. Leipzig: S. Hierzel. J.A Dent, A.G. Polson, M.W. Klymkowsky....1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et ... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m.... peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant...Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS 1.8 Solubility and Affinity Abundance... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog PostPublishedNov. 12, 2014, 3:54 p.m....CRISPR/Cas9 vector system. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T. Sci Rep. 2014 Jun 23;4:5400... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m.... then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015)....al., 2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression...//doi.org/10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling... -
What's New in CRISPR - Winter 2018
TypeBlog PostPublishedDec. 18, 2018, 1:17 p.m....precise deletion products compared to monomeric Cas9’s. Read the paper in Nature Communications Find ... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....nucleotides of DNA can serve as the target for Cas9’s single guide RNA (sgRNA), provided it meets two conditions...system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic residues has been converted... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....Matho, K. S., Cetin, A., Woodard, K., Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic...Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization...