Skip to main content
Addgene
Showing: 181 - 200 of 343 results

  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...identified that a S375A mutation in RESCUE (named RESCUE-S) offered ~76% on-target C-to-U editing while reducing...from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...

  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even temperature change... all of the droplets, but you can go as low as 1C/s. Increasing the elongation time to 2 minutes and the...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...You’ll first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to ...Barnett, Jai A. Pathak, Christopher J. Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation...

  4. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ... to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination...doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of Lentiviral...

  5. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...References and Resources References Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences...Sciences Education. 15(4):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome... this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ... Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros...Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318-22....

  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...transferable. Use your experience and your passion(s) to guide you in building a grant career. Do you have... client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor, find...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... menschlichen und tierischen Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky....1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et ...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant...Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS  1.8 Solubility and Affinity Abundance...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015)....al., 2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression...//doi.org/10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling...

  13. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...precise deletion products compared to monomeric Cas9’s. Read the paper in Nature Communications Find ...

  14. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic...Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization...

  15. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

  17. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...nucleotides of DNA can serve as the target for Cas9’s single guide RNA (sgRNA), provided it meets two conditions...system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic residues has been converted...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences,...They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t integrate or replicate...from this reference. 1. Malik, Nasir, and Mahendra S. Rao. "A Review of the Methods for Human iPSC Derivation...

  20. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...://doi.org/10.7554/elife.06100 Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings...
Showing: 181 - 200 of 343 results