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We narrowed to 431 results for: {{{s}}}

Showing: 1 - 20 of 431 results

  1. Kit Free RNA Extraction

    Type
    Protocol
    ...seconds. Incubate sample(s) for 15 minutes on ice and centrifuge the sample(s) for 15 minutes at 12,000...mL of Solution D per 1 X 10 7 cells. Allow sample(s) to sit at room temperature for 5 minutes to allow...the cells. Extract RNA from the homogenized sample(s).Transfer tissue/cell lysate to a 4 mL tube. Add the...aiming your tip in the tube. Precipitate your sample(s). You can use either Isopropanol or Lithium Chloride...Quantify and assess the quality of your RNA sample(s) using a spectrophotometer (such as a Nanodrop), agarose...which can be modified for RNA. Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple... 1 mL of TRIzol® per 1 X 10 7 cells. Allow sample(s) to sit at room temperature for 5 minutes to allow...

  2. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...tube combine the following: DNA Restriction Enzyme(s) Buffer BSA (if recommended by manufacturer) dH 2 ...purifying it, you may need to inactivate the enzyme(s) following the digestion reaction. This may involve...digesting a large number of plasmids with the same enzyme(s) (for instance, in a diagnostic digest), you can create...

  3. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...tube of 6 mL BCD TFX. Cap the tube and vortex for 5 s to mix. Add 450 µL of 1 mg/mL PEI-MAX to the second..., 450 µg = 450 µL). Cap the tube and vortex for 5 s to mix. Pro-Tip The optimal ratio of DNA:PEI may vary...diluted DNA. Cap the tube and vortex with three 1 s pulses. Incubate for 3 min at room temperature. Transfer...

  4. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ... size insert. By selecting the appropriate enzyme(s), one can either linearize a plasmid to determine ...therefore the only thing left to do is identify the clone(s) in which the insert is in the correct orientation...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...none S. pyogenes Gao pZmU3-gRNA 53061 Plant none S. pyogenes Gao pU6-gRNA 53062 Plant BbsI none S. pyogenes...vector was designed to be used with, such as S. pyogenes, S. aureus, N. meningitidis, etc. gRNA scaffolds...Bacteria BsaI none S. pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol...HindIII none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein.... elegans BsaI none S. pyogenes Joung pCFD3-dU6:3gRNA 49410 Drosophila BbsI none S. pyogenes Virmilion...BspQI yes, cut S. pyogenes Puro Liu pCFD4-U6:1_U6:3tandemgRNAs 49411 Drosophila BbsI none S. pyogenes Virmilion...Mammalian AfIII none S. pyogenes Bleocin Church MLM3636 43860 Mammalian BsmBI none S. pyogenes Joung pSPgRNA...

  6. CRISPR Guide

    Type
    Collection
    ...23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou,...Ishiguro, S., Gao, L., Hirano, S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H...Chandrasekaran, S. S., Perry, N. T., Schaepe, J., Du, P. P., Lotfy, P., Bassik, M. C., Bintu, L., Bhatt, A. S., & ...PMID: 38216671 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...Scheiman, J., Vora, S., Pruitt, B. W., Tuttle, M., Iyer, E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand..., E. C., Newberry, K. M., Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E....Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung...

  7. COVID-19 Resources

    Type
    Collection
    ...Kleine-Weber, H., Schroeder, S., Krüger, N., Herrler, T., Erichsen, S., Schiergens, T. S., Herrler, G., Wu, N....key step is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed...-2 Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain..., Hofmann-Winkler, H., Gierer, S., Liepold, T., Jahn, O., & Pöhlmann, S. (2014). TMPRSS2 and ADAM17 cleave... MERS CoV, depends on binding of the viral spike (S) protein to cellular receptors. The cellular receptor...genes encoding SARS-CoV-2 proteins, (spike protein, S; small envelope protein, E; nucleocapsid protein, ...plasmids - a serine protease that primes the SARS-CoV-2 S protein and is involved in virus entry into cells ...

  8. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...30783202 (Link opens in a new window) Lee, S., Lim, W. A., Thor,n K. S. (2013) Improved blue, green, and red...red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https://doi.org/10.1371... (Link opens in a new window) Loqué, D., Lalonde, S., Looger, L. L., von Wirén, N., Frommer, W. B. (2007...Link opens in a new window) Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein...Link opens in a new window) Vickers, C. E., Bydder, S. F., Zhou, Y., Nielsen, L. K. (2013). Dual gene expression...

  9. Recombinases AAV Preps

    Type
    Collection
    ... ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Hongkui Zeng... ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA Syn none rg* Hongkui Zeng ...Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII...Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none 1, PHPeB Hongkui...VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a none 8, rg* Karl Deisseroth...

  10. The Pleiades Promoter Project

    Type
    Collection
    ...Jiang, S., Khorasan-zadeh, S., Komljenovic, I., Laprise, S., Liao, N. Y., Lim, J. S., Lithwick, S., Liu..., Wong, B. K., Wong, S. H., Wong, T. Y., Yang, G. S., Ypsilanti, A. R., Jones, S. J., Holt, R. A., Goldowitz... Ho Sui, S. J. Fulton, D. L., Ali, J., Amirabbasi, M., Arenillas, D. J., Babyak, N., Black, S. F., Bonaguro...selective expression in the brain. Proc Natl Acad Sci U S A, 107 (38):16589-94. https://doi.org/10.1073/pnas...

  11. CRISPR References and Information

    Type
    Collection
    ...identifies putative target sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis...checks for off-target binding and can work for S. pyogenes , S. thermophilus , or N. meningitidis Cas9 PAMs...support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ), fruit fly, zebrafish...to maximize on-target activity for selected target(s). Uses human, mouse, and rat reference genomes. Hosted...forum started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol...gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49 KB Vosshall and Matthews (Link opens in a...

  12. p53 Pathway

    Type
    Collection
    ...ageing-associated phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G...papillomavirus). These mutations interfere with p53’s ability to activate transcription, and they also have...through oligomerization. In particular, the loss of p53’s pro-apoptotic effects is especially important to tumorigenesis...kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed 1; also known as GTSE1 BAI-1 Brain-specific... human malignancy: a clinical perspective. Surget S, Khoury MP, Bourdon JC. Onco Targets Ther. 2013 Dec...

  13. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...use of phenol red. Image adapted from Zolotukhin, S., et al. "Recombinant adeno-associated virus purification...purified AAV. Left image adapted from Zolotukhin, S., et al. "Recombinant adeno-associated virus purification...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...(monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish Xenopus References Barrangou... Acad Sci U S A . 112(10):3002-7. PMID: 25713381 Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D...and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16 subtypes based...PAM, improving targeting in AT-rich genomes. Cpf1's small size also makes it suitable for multiplexing...Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero DA, Horvath P. 2007. CRISPR provides acquired...12. PMID: 17379808 Bondy-Denomy J, Garcia B, Strum S, Du M, Rollins MF, Hidalgo-Reyes Y, Wiedenheft B, ...407-410. PMID: 28931002 Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...

  15. Zhang Lab CRISPR Page

    Type
    Collection
    ... CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell . 2013...Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial nuclease...implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease along with the guide...The single vector system uses a smaller Cas9 from S. aureus (SaCas9) that has been human codon-optimized...et al., Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express...using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick...

  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Aguiar, M., Beausoleil, S. A., Paulo, J. A., Rinehart, J., Huttlin, E. L., & Gygi, S. P. (2022). A multi-... described in: Mohler, K., Moen, J. M., Rogulina, S., & Rinehart, J. (2023). System‐wide optimization ...Barber, K. W., Muir, P., Szeligowski, R. V., Rogulina, S., Gerstein, M., Sampson, J. R., Isaacs, F. J., & Rinehart...Aerni, H. R., J, N., MA, Haimovich, A. D., Rogulina, S., Isaacs, F. J., & Rinehart, J. (2015). A flexible... N. L., Barber, K. W., Ter Haar, C. M., Rogulina, S., Amrofell, M. B., Isaacs, F. J., Rinehart, J., & ...Heinemann, I. U., Rovner, A. J., Aerni, H. R., Rogulina, S., Cheng, L., Olds, W., Fischer, J. T., Söll, D., Isaacs...

  17. Deisseroth INTRSECT Collection

    Type
    Collection
    ...) Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, Ma T, Ztaou S, Sienna AC, Tepler S, Poulin JF, Ansorge...Link opens in a new window) Mingote S, Amsellem A, Kempf A, Rayport S, Chuhma N. 2020. Dopamine-glutamate...Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed...Preprint (Link opens in a new window) Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, Deisseroth...Ramakrishnan C, Fenno L, Deisseroth K, Herry C, Arber S, Lüthi A. 2016. Midbrain circuits for defensive behaviour...

  18. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Ben-Simon, Y., Hooper, M., Narayan, S., Daigle, T. L., Dwivedi, D., Way, S. W., Oster, A., Stafford, D. A....-Araya, X., Roth, J. R., Alexander, J. R., Allen, S., Amster, A., Arbuckle, J., Ayala, A., Baker, P. M...Bendrick, J. L., Kim, T. K., Zhou, T., Mortrud, M., Yao, S., Siverts, L. A., Larsen, R., Gore, B. B., Szelenyi...Johansen, N. J., Hooper, M., Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird, W. D....Ben-Simon, Y., Opitz-Araya, X., Martinez, R. A., Way, S. W., Thyagarajan, B., . . . Ting, J. T. (2025). Enhancer...Mahoney, J. T., Kojima, Y., Ding, Y., Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli, C., Gore...Shapovalova, N. V., Martinez, R. A., Fong, O., Yao, S., Mortrud, M., . . . Levi, B. P. (2021). Functional...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...Gossen, M., Bujard, H., & Reed, S. I. (1994). Acceleration of the G1/S phase transition by expression ...transactivator (rtTA-Advanced, also known as rtTA2 S -M2) with greater sensitivity to doxycycline and three...tetracycline-responsive promoters . Proc Natl Acad Sci U S A, 89 (12), 5547–5551. https://doi.org/10.1073/pnas...Link opens in a new window) Gossen, M., Freundlieb, S., Bender, G., Müller, G., Hillen, W., & Bujard, H....PMID: 8114703 (Link opens in a new window) Urlinger, S., Baron, U., Thellmann, M., Hasan, M. T., Bujard, ...expanded range and sensitivity . Proc Natl Acad Sci U S A, 97 (14), 7963–7968. https://doi.org/10.1073/pnas...
Showing: 1 - 20 of 431 results