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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 704 results for: ANG

Showing: 181 - 200 of 704 results

  1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... to create xCas9, an enzyme recognizing a broad range of PAMs like NG, GAA, and GAT, but also displaying...spectinomycin resistance. xCas9 3.7 showed the broadest PAM range, recognizing NG, NNG, GAT, and CAA PAMs, while xCas9... xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian cells Hu et al. compared... the observed off-target loci displayed a broad range of PAM sequences, indicating they may be less predictable... with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses. The development...

  2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such as the human gut bacteria...proteins. Second, it can be introduced into a broader range of bacteria. Once transferred, Mobile-CRISPRi stably...reporter, was variable across bacterial strains, ranging from an ~8 to 150-fold reduction, with a median...been a target for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible...27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system." Nature...

  3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ..., a deletion or a frameshift (Ding et al., 2014, Wang et al., 2015). In these studies, the strategy employed...disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al., 2014). An alternative...and Adeno EA. Or if you're looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423... . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin...

  4. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...enjoy seeing how our quality control process has changed with the introduction of next generation sequencing... we use blastn to identify any discrepancies in Sanger sequences, such as mismatches, deletions, or insertions... first decision to make is whether to compare a Sanger sequencing result to a single known reference sequence...95% of our results were always correct!]  Since Sanger sequencing reactions are imperfect and sequence...used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential mismatches...

  5. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...instead of nMagHigh1-C714, generating paCas9-2. This change did not significantly alter the system’s efficiency...Using Guide RNAs with Photocleavable Protectors. Angew Chem Int Ed 55:12440–12444 . https://doi.org/10.1002...Biology. Springer US, pp 261–281 Manna D, Maji B, Gangopadhyay SA, Cox KJ, Zhou Q, Law BK, Mazitschek R, Choudhary...Dosing and Spatiotemporal Control of CRISPR‐Cas9. Angew Chem Int Ed 58:6285–6289 . https://doi.org/10.1002...Bardhan A, Delaney M, Ilk AS, Rauen RR, Kahn SI, Tsang M, Deiters A (2020) Spatiotemporal Control of CRISPR... and Zebrafish using Light‐Activated Guide RNA. Angew Chem Int Ed 59:8998–9003 . https://doi.org/10.1002...

  6. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...have larger homology arms in the 500 bp to 1 kb range, however, shorter homology tracts have recently ...is a silent mutation, so you don’t accidentally change your protein of interest.   Fig. 2: Stop...to be efficacious for increasing knock-in events (Yang et al., Int J Mol Sci). Adding these inhibitors ...cutting. Cas12a may promote HDR by generating ssDNA overhangs at the break site – an early HR step (Moreno-Mateos...: 10524-10535 (2018). 1 10.1074/jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring...

  7. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...10.1073/pnas.76.2.615  Zhou, Y., Zhang, Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018...the Patterson Protocol was shown to purify DNA ranging in size from 100 bp to 48 kb with high yields and...10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G., Wang, Y., Wee, E.J., Turni, C., Blackall, P.J., Trau,...

  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...regulations. A grant professional’s job duties can range from coordinating the grant application and submitting...government grantmakers. We keep up-to-date on the changing guidelines and regulations impacting grant management...government. Throughout these moves and program changes, I learned to write grants for health, housing,...within the city infrastructure. My career path changed when I was asked if I would consider consulting...evaluation and grant management.  Your niche will change over time. Going into the grants profession with...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...technology? See our improved CRISPR-Cas Guide. Wang et al., Science. 2014. Jan 3;343(6166):80-4. Shalem...a mutant of the fluorescent protein dsRed that changes irreversibly its color from green to red when it... show its usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer...green) protein and organelles with older (yellow to orange) protein. Image courtesy of Roberta Gottlieb. ...sites, and provides a modular system for easier exchange and re-use of existing elements. DNA sequences... and Dieter Söll at Yale University previously changed the way researchers can explore important questions...site, and permits DNA fragments with compatible overhangs to be efficiently assembled. Scientists can engineer...

  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... could be screened for their affinity to a wide range of non-nucleotide targets. These RNA molecules, ...The key to aptamers’ flexibility is their broad range of targets, including proteins, peptides, amino ...ions, and cells (even pathogenic bacteria). That range is possible because aptamers can either fold to ...- since they frequently undergo conformational changes after target binding, aptamers are highly customizable... 26877022. PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent...

  11. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...of diagnosis) is about 35 years, but it usually ranges from 20-50 years and sometimes longer. There is...mesothelioma is so rare, there’s not really much of a danger of getting the disease, even if you do happen to...options: Early detection, immunotherapy, and angiogenesis inhibitors New research is also going into two...bevacizumab (Avastin). This drug (known as an angiogenesis inhibitor) prevents the growth of new blood ...future generations will be protected from this dangerous material. This post was contributed by the Mesothelioma...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...Lukas Villiger, Wenyuan Zhou, Kaiyi Jiang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, John...broadly similar to shRNA and Cas13 systems in a range of mammalian cell lines. However, in those same ...Prime editing — the ability to now make any small changes, including base edits, insertions, and deletions...

  13. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...doi.org/10.1089/hgtb.2017.084 Li Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development...virus to either restrict or broaden the host cell range (tropism). Pseudotyping is only done on enveloped...513 . https://doi.org/10.3390/v12050513 Cronin J, Zhang X-Y, Reiser J (2005) Altering the Tropism of Lentiviral... Jennifer Tsang...

  14. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from....0029875 Pubmed PMID: 22253811 Ni B, Du Z, Guo Z, Zhang Y, Yang R (2008) Curing of four different plasmids in...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...to simultaneously test how multiple amino acid changes affect a protein of interest’s function. This technique...combination and possibly discover unexpected epistatic changes in protein activity. See Natarajan et al for an...mutagenic primers to introduce all desired codon changes (step 1 in the image above). A second universal...This results in the final product having a codon change present on both strands of the plasmid. The ssDNA...PMC4409680. 2. Mccullum, E. O., Williams, B. A., Zhang, J., & Chaput, J. C. (2010). “Random Mutagenesis...

  16. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ... technology. Links: Zhang Lab’s CRISPR FAQs CRISPR forum moderated by the Zhang lab CRISPR Challenges... aging, and other harmful processes. The most dangerous type of DNA lesion results from breakage of both... is resected by nucleases to create a ssDNA 3’ overhang. This will serve as both a substrate for proteins...or on the non-crossing strand (vertically at the orange arrows). If resolved dissimilarly (e.g. one junction...

  17. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...How are you currently exchanging information with your team? If you need to exchange information regarding...types of ELNs are available on the market today, ranging from general note taking tools, to more scientific....e. main priorities of different ELN features, ranging from whether respondents saw them as not important...which they are sure that there will be no sharp changes in costs once they establish the system within ...

  18. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...10.7554/elife.06100 Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian...doi.org/10.1159/000504294 Wang, T., Guan, C., Guo, J., Liu, B., Wu, Y., Xie, Z., Zhang, C., & Xing, X.-H. (...addition, axolotls have many age-related phenotypic changes that are similar to those observed in humans. Thus...

  19. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated...the region you want to cover - and as your titer changes, so may your needed volume (de Backer et al., 2010...

  20. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...publication at Addgene.  Lei Y, Zhang X, Su J, Jeong M, Gundry MC, Huang Y-H, Zhou Y, Li W, Goodell MA ... at Addgene. Xu X, Tao Y, Gao X, Zhang L, Li X, Zou W, Ruan K, Wang F, Xu G, Hu R (2016) A CRISPR-based... from this publication at Addgene. Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai ...
Showing: 181 - 200 of 704 results