We narrowed to 711 results for: ANG

...precise insertion of a DNA sequence ranging from a single nucleotide change to large insertions like the addition...: The key components of Nanoblades. Image from Mangeot et al., 2019.   MLV GAG-POL polyprotein Nanoblades...efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar to other approaches... accessible genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary...

...may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying the...working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition of the viral...viral vector with bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain... following transfection. In other words, when exchanging the medium after transfecting cells, replace ...

... to create xCas9, an enzyme recognizing a broad range of PAMs like NG, GAA, and GAT, but also displaying...spectinomycin resistance. xCas9 3.7 showed the broadest PAM range, recognizing NG, NNG, GAT, and CAA PAMs, while xCas9... xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian cells Hu et al. compared... the observed off-target loci displayed a broad range of PAM sequences, indicating they may be less predictable... with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses. The development...

..., a deletion or a frameshift (Ding et al., 2014, Wang et al., 2015). In these studies, the strategy employed...disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al., 2014). An alternative...and Adeno EA. Or if you're looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423... . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin...

...bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such as the human gut bacteria...proteins. Second, it can be introduced into a broader range of bacteria. Once transferred, Mobile-CRISPRi stably...reporter, was variable across bacterial strains, ranging from an ~8 to 150-fold reduction, with a median...been a target for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible...27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system." Nature...

...in other plant species, thanks to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T.,...transsynaptic label based on antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based...subunit GluA1 found at excitatory synapses (Rivera & Huang, et al., 2025). AF was fused to a presynaptic vesicle...generated with mRNA display. Modified from Rivera & Huang, et al. (2025) under CC BY-NC-ND license, created...applications. Find ATLAS plasmids here! Rivera, J. F., Huang, H., et al. (2025). ATLAS: a rationally designed...

...enjoy seeing how our quality control process has changed with the introduction of next generation sequencing... we use blastn to identify any discrepancies in Sanger sequences, such as mismatches, deletions, or insertions... first decision to make is whether to compare a Sanger sequencing result to a single known reference sequence...95% of our results were always correct!]  Since Sanger sequencing reactions are imperfect and sequence...used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential mismatches...

...instead of nMagHigh1-C714, generating paCas9-2. This change did not significantly alter the system’s efficiency...Using Guide RNAs with Photocleavable Protectors. Angew Chem Int Ed 55:12440–12444 . https://doi.org/10.1002...Biology. Springer US, pp 261–281 Manna D, Maji B, Gangopadhyay SA, Cox KJ, Zhou Q, Law BK, Mazitschek R, Choudhary...Dosing and Spatiotemporal Control of CRISPR‐Cas9. Angew Chem Int Ed 58:6285–6289 . https://doi.org/10.1002...Bardhan A, Delaney M, Ilk AS, Rauen RR, Kahn SI, Tsang M, Deiters A (2020) Spatiotemporal Control of CRISPR... and Zebrafish using Light‐Activated Guide RNA. Angew Chem Int Ed 59:8998–9003 . https://doi.org/10.1002...

...10.1073/pnas.76.2.615  Zhou, Y., Zhang, Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018...the Patterson Protocol was shown to purify DNA ranging in size from 100 bp to 48 kb with high yields and...10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G., Wang, Y., Wee, E.J., Turni, C., Blackall, P.J., Trau,...

...have larger homology arms in the 500 bp to 1 kb range, however, shorter homology tracts have recently ...is a silent mutation, so you don’t accidentally change your protein of interest.   Fig. 2: Stop...to be efficacious for increasing knock-in events (Yang et al., Int J Mol Sci). Adding these inhibitors ...cutting. Cas12a may promote HDR by generating ssDNA overhangs at the break site – an early HR step (Moreno-Mateos...: 10524-10535 (2018). 1 10.1074/jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring...

...regulations. A grant professional’s job duties can range from coordinating the grant application and submitting...government grantmakers. We keep up-to-date on the changing guidelines and regulations impacting grant management...government. Throughout these moves and program changes, I learned to write grants for health, housing,...within the city infrastructure. My career path changed when I was asked if I would consider consulting...evaluation and grant management.  Your niche will change over time. Going into the grants profession with...

...technology? See our improved CRISPR-Cas Guide. Wang et al., Science. 2014. Jan 3;343(6166):80-4. Shalem...a mutant of the fluorescent protein dsRed that changes irreversibly its color from green to red when it... show its usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer...green) protein and organelles with older (yellow to orange) protein. Image courtesy of Roberta Gottlieb. ...sites, and provides a modular system for easier exchange and re-use of existing elements. DNA sequences... and Dieter Söll at Yale University previously changed the way researchers can explore important questions...site, and permits DNA fragments with compatible overhangs to be efficiently assembled. Scientists can engineer...

...Lukas Villiger, Wenyuan Zhou, Kaiyi Jiang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, John...broadly similar to shRNA and Cas13 systems in a range of mammalian cell lines. However, in those same ...Prime editing — the ability to now make any small changes, including base edits, insertions, and deletions...

...doi.org/10.1089/hgtb.2017.084 Li Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development...virus to either restrict or broaden the host cell range (tropism). Pseudotyping is only done on enveloped...513 . https://doi.org/10.3390/v12050513 Cronin J, Zhang X-Y, Reiser J (2005) Altering the Tropism of Lentiviral... Jennifer Tsang...

....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from....0029875 Pubmed PMID: 22253811 Ni B, Du Z, Guo Z, Zhang Y, Yang R (2008) Curing of four different plasmids in...

... could be screened for their affinity to a wide range of non-nucleotide targets. These RNA molecules, ...The key to aptamers’ flexibility is their broad range of targets, including proteins, peptides, amino ...ions, and cells (even pathogenic bacteria). That range is possible because aptamers can either fold to ...- since they frequently undergo conformational changes after target binding, aptamers are highly customizable... 26877022. PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent...

...of diagnosis) is about 35 years, but it usually ranges from 20-50 years and sometimes longer. There is...mesothelioma is so rare, there’s not really much of a danger of getting the disease, even if you do happen to...options: Early detection, immunotherapy, and angiogenesis inhibitors New research is also going into two...bevacizumab (Avastin). This drug (known as an angiogenesis inhibitor) prevents the growth of new blood ...future generations will be protected from this dangerous material. This post was contributed by the Mesothelioma...

...How are you currently exchanging information with your team? If you need to exchange information regarding...types of ELNs are available on the market today, ranging from general note taking tools, to more scientific....e. main priorities of different ELN features, ranging from whether respondents saw them as not important...which they are sure that there will be no sharp changes in costs once they establish the system within ...

...10.7554/elife.06100 Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian...doi.org/10.1159/000504294 Wang, T., Guan, C., Guo, J., Liu, B., Wu, Y., Xie, Z., Zhang, C., & Xing, X.-H. (...addition, axolotls have many age-related phenotypic changes that are similar to those observed in humans. Thus...

... technology. Links: Zhang Lab’s CRISPR FAQs CRISPR forum moderated by the Zhang lab CRISPR Challenges... aging, and other harmful processes. The most dangerous type of DNA lesion results from breakage of both... is resected by nucleases to create a ssDNA 3’ overhang. This will serve as both a substrate for proteins...or on the non-crossing strand (vertically at the orange arrows). If resolved dissimilarly (e.g. one junction...