Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 393 results for: ARA-2

Showing: 181 - 200 of 393 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... Genes Dev. 1988 Jun;2(6):718-29. doi: 10.1101/gad.2.6.718. PMID: 2843425.Takara Bio. Tet-On and Tet-Off...Targeted Gene Expression in Drosophila.” Genetics 186(2) (2010): 735-55. PubMed PMID: 20697123. PubMed Central... in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197-214. PubMed PMID: 22129153. Additional... the system have been engineered and improved. Takara’s “2nd generation Tet-Off vectors (Tet-Advanced)...this way, you can interrogate the activity of uncharacterized promoters. Similarly, placing UAS upstream ...-Off systems: Second generation. https://www.takarabio.com/products/gene-function/tet-inducible-expression-systems...

  2. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... pCRISPomyces-2 contains Cas9 and a gRNA cassette. The simpler system of pCRISPomyces-2 displays a higher...and a subsequent curing protocol that requires only 2-3 hours incubation. E. coli and T. citrea scarless...four major components including (1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting ...expresses lambda Red and Cas9, which are separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively... template or non-template strand, among other parameters. .  Stanley Qi lab's CRISPRi plasmids Stanley...across multiple gRNAs per gene (Singh et al. 2016). Sarah Fortune's lab M. tuberculosis CRISPRi plasmids Noting...proteotoxic when used in Mycobacterial species, Sarah Fortune’s lab screened 11 other Cas9 orthologs to...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...nonsense-mediated decay. Although Nelson et al. observed only 2% genome editing in one experiment, they found the ... cells throughout the body, especially stem cells 2. Remove any immunogenicity of the AAV vector Once ...Science. 2015 Dec 31. doi:10.1126/science.aae0169 2. Long C, Amoasii L, Mireault AA, McAnally JR, Li H...highest-ranking predicted off-target sites. Each paper characterizes additional, unique facets of CRISPR DMD therapy...effectiveness and off-target risk must be evaluated separately, increasing the time to clinical approval. Even...Blog Learn How CRISPR Could Be Used to Treat Cataracts and Cystic Fibrosis Read How Homology Directed...

  4. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...wisdom  Reach out to 2-3 individuals in your professional circle to vouch for the character you painted in ...and scholarships in different subject areas!    2. Resume and cover letter know-how  Tailor your resume...increase your competitive edge, they alone, are no guarantee of success. Don’t luck into an internship, prepare...

  5. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast episode 2 "Essential...flagging passive voice, overuse of adverbs, and paragraphs with many hard-to-read sentences.  Grammarly ...

  6. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...linear fashion. This implies that reducing the power 2-fold does not reduce photobleaching by half. How exactly...prolonged time). Other illumination strategies, such as 2-photon-excitation, selective plane illumination, TIRF...al, 2017) and cannot be described by a single parameter. Therefore, it is necessary to know how the fluorescence... power, the photostability of the variants is comparable (lower panel of figure 1). To avoid changes in...microscopy (University of Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes...

  7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...studies have shown that the conditions Sotos-type 2 and Marshall Smith Syndrome are due to mutations in...accurate diagnosis of NFIX-related syndromes (Sotos-type 2 and Marshall-Smith) and could be expanded to NFIA-...manifestations. PMM2-CDG is the most frequent CDG type characterized by deficient phosphomannomutase (PMM) activity...suffer from immunological defects. These are characterized by a predisposition to severe and recurrent ... in the NFIX gene. These diseases are characterized by overgrowth phenotypes and neural developmental ...

  8. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    Published
    Dec. 5, 2013, 3:27 p.m.
    ...   1)   “Piled Higher and Deeper” by Jorge Cham   2)   “Boxplot” by Maki Naro   3)   “Bird and Moon” by...Unearthed Comics” by Sara Zimmerman   5)   “Beatrice the Biologist” by Katie McKissick       Sara Zimmerman, Unearthed...

  9. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    Published
    March 16, 2016, 5:30 p.m.
    ...reach out to the corresponding potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid...where we can find the plasmid. While we cannot guarantee that everyone we reach out to will end up depositing...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ..., and the desired gene (transfer vector plasmid), 2) the HIV-1 genome containing all viral genes with ...protocols that have been described (protocol 1, protocol 2, protocol 3) to find the one that will give you the...PubMed PMID: 3785217. PubMed Central PMCID: PMC367857. 2. Naldini, Luigi, et al. "In vivo gene delivery and...safer system. Unlike the MoMLV, lentiviruses, a separate genus of the Retroviridae family including human...facilitates nuclear export) is expressed from a separate plasmid, and the promoter of the 5’LTR has been... In this case your gene and the marker can be separated by an internal ribosome entry site (IRES) and ...

  11. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...residues, which can be found throughout the antibody. 2) Endogenous cysteines, such as those that make up ...Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/nprot.2007.267 ...commercially conjugated antibodies available, that never guarantees the antibody you need will be conjugated, or ...//doi.org/10.3390/antib4030197  Hegazy M, Cohen-Barak E, Koetsier JL, et al (2020) Proximity Ligation ...

  12. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...misplacing your results or losing that lab notebook from 2 years ago. The free subscription to Lab Archives allots...notebooks you’re saving trees! The Earth thanks you. 2. Gibco Cell Culture by Gibco Whenever I start to learn...MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet is in this...

  13. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... reduce the background staining (Table 2).      Table 2: Secondary antibody selections for a four-antibody...with users trying to probe multiple targets in parallel. In these cases, it is critical that users choose... experiment.  When probing multiple targets in parallel, use the most narrowly specific secondary antibodies... four-antibody panel, staining in parallel    In short, when staining multiple targets, look for primary...

  14. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...days before the deadline isn’t going to be much use. 2. Originality is essential for grant success The two... it’s important not to throw in the towel if 1 or 2 funding bodies say no. If possible, get feedback from...perfectly reasonable paragraph unintelligible to a lay audience. My lay paragraph typically includes a...kindly friends and family to read my non-expert paragraph and point out words or sentences that are unclear...

  15. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ... signal back again. The Ting lab constructed 2 versions of this reporter to target the K9 (pcDNA3-K9 ... 525.7570 (2015): 533-537. PubMed PMID: 26352475. 2. Bygren, Lars O., et al. "Change in paternal grandmothers...disrupts normal function. Angelman syndrome is characterized by intellectual and developmental disability...or smiling, whereas Prader–Willi syndrome is characterized by low muscle tone, short stature, cognitive... in the FRET signal. Subsequent demethylation separates the fluorescent units and so lowers the FRET signal...

  16. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...populations that may otherwise be difficult to separate out. In Figure 2, you can see that, without the FMO, it... from the Ly6C positive population. Figure 2: An FMO gating example. The FMO sample (A) can be ...

  17. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...represent a favorable state for tumor progression (Ref 2). Future treatments may need to target this immunosuppressive...cell 30.1 (2016): 147-160. PubMed PMID: 27411589. 2. Todoric, Jelena, Laura Antonucci, and Michael Karin...the Relationship” by our guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... Zetsche et al., Nat Biotechnol. 2015 Feb 2;33(2):139-42. doi: 10.1038/nbt.3149. Light-inducible ...and on your favourite signalling pathways (Figure 2). They are all available now at Addgene. 1 Lungu...20;19(4):507-17. 10.1016/j.chembiol.2012.02.006. 2 Guntas et al., Proc Natl Acad Sci USA 2015 Jan 6; ...Nihongaki et al., Chemistry and Biology. 2015 Feb 19; 22(2):169-74. High efficiency cloning for enzyme screening...inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene deletion, (3) simultaneous...different fluorescent proteins, 8 constitutive promoters, 2 includible promoters, 3 polyA terminators and various...of plasmids required for each input/output system; 2) removing all chemically inducible promoters; 3) improving...

  19. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...knock-out cell line to perform downstream characterization of, isolating 2 or 3 distinct clones to perform key...sites would confound your experimental results and 2) perform targeted off-target measurements at those...experiments where only localization of the Cas occurs. 2 gRNA approach Off-targets that come from mutations...

  20. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...somewhere else (Fig. 2). Class II TEs have LTRs on both of their ends.    Figure 2: Overview of DNA...has four major components: 1) a CRISPR RNA (crRNA), 2) four proteins (TniQ, Cas8, Cas7, Cas6) that forms...another. This jumping is what caught the eye of Barabara McClinntock who first discovered transposons due...
Showing: 181 - 200 of 393 results