Skip to main content
Addgene
Showing: 181 - 200 of 455 results

  1. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor...

  2. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...vectors VSV-G and psPAX2, which were used in a publication by Xiuyuan Ou, et al. that characterized the ... requested these plasmids from Addgene. This publication then resulted in the development of a new plasmid...

  3. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...do just this.  In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with...

  5. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate in the biotech revolution...detect compounds in the environment, and openly publish protocols so that anyone can engineer yeast to ...

  6. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...expect to generate data that will contribute to a publication? A grant? A patent? It is never too early to ...

  7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...this screen successfully differentiated cells resembling all three germ layers (ectoderm, endoderm, and...amplification, refer to the Zhang lab’s previously published protocol and its relevant modifications on the...

  11. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...nucleofection The in vivo editing experiments in this publication were performed using a technique called nucleofection...Additonal Corn Lab Members who worked on this publication:         References 1. Richardson, Christopher...

  12. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...available. With new fluorescent proteins being published every month, deciding on the best protein for ...common use (particularly not the most recently published ones), they can be a good starting point. In many...

  13. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...In the blink of an eye, the long days in the lab as a graduate student and postdoc come to an end and...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane...

  14. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...to make the sequences of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative...Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded their recombinant...

  15. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...

  16. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...resulting in a fluorescent signal. Since their first publication in 2012, the Nagai laboratory has assembled a...sensitivity and faster imaging compared to previous publications. They also developed calcium, cAMP, and ATP ...

  17. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

  18. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

  19. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking about these...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...

  20. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...to find the simplest way to do it (i.e. avoid assembling too many fragments at once, if it is possible...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...
Showing: 181 - 200 of 455 results