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We narrowed to 569 results for: CHI;

Showing: 181 - 200 of 569 results

  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques ...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

  2. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...while not widely known, plasmid assembly can be achieved in vivo using a bacterial recombination pathway...in a gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications requires multiple recombination...DNA encoding plasmids, or promoters such as the chicken beta-actin promoter of the pHLsec vector. In this...

  4. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing cells....convert adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit...as assessed using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital PCR as a tool for...is common. Here are some steps you can take to achieve a clean NTC: Have a dedicated bench with a dedicated...

  6. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...responsible for an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital... therapy restores vision in a canine model of childhood blindness. Nature Genetics 28, 92-95. PubMed PMID...PubMed Central PMCID: PMC5960719. 8. Trapani I and Auricchio A., 2018. Seeing the Light after 25 Years of Retinal...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...was derived from figure 3b in Chan et al.   To achieve the full diversity of colors AND sparse labeling...co-injected. The dose of this AAV can be adjusted to achieve sparse levels of labeling, without loss of color...virus, but the desired sparsity of labeling is achieved with lose doses of inducer virus. Additionally...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate the...bacteria have poor non-homologous end joining machinery, so a CRISPR-induced double-stranded break (DSB...into the bacterial genome, and it carries CRISPR machinery targeting 1) the given resistance gene and 2) ...

  9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...and the viral mRNAs are translated) by cellular machinery. NP encapsidates the negative sense, single-stranded.... These mRNAs are translated by cellular host machinery (as viral mRNAs would be in the course of viral...initial titration experiments might be helpful to achieve optimal results. It is important to note that this...

  10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...happening! Addgene actually has chimes outside of our break room. A ring of the chimes means something fun is ...completed their work thoroughly. It’s kind of a chicken and egg situation- do we work so hard so we have...

  11. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...If you are job searching, you may be wondering if you need to go to the effort of writing a cover letter...relocation plans. They can be skills-based: if you're switching fields, you can use your cover letter to explain...greeting like “Dear Hiring Manager.” Highlight your achievements with specific examples. Instead of saying “I...

  12. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...leading to a positive signal. The same effect can be achieved for a fluorophore that relies on dimerization ...cleavage and an RNA aptamer that bound the compound malachite green (MG) to create a non-protein based visualization...of high throughput assays with microarrays and biochips. Ultimately, there are just some applications ...

  13. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...salaries). Postdocs (who are not in a primarily teaching role) come under this ruling, regardless of visa...on higher education, you can see that those in teaching roles (such as adjuncts) and graduate and undergraduate...already have salaries at or above $50,000. Boston Children’s Hospital is the only part of the Harvard group...

  14. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...projects. Almost all of my projects, have involved enriching conversations with R&D, Medical Affairs, and Commercial...challenges, quickly breaking them up into smaller achievable tasks, and then executing on them in a timely...situations, and personalities. Teams striving to achieve a lot of great output in a short amount of time...

  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...a synonymous mutation can be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall, codon optimization may... A, Santana-Quintero LV, Katneni U, Simonyan V, Kimchi-Sarfaty C (2017) A new and updated resource for... https://doi.org/10.1093/nar/28.1.292 Sauna ZE, Kimchi-Sarfaty C (2011) Understanding the contribution...

  16. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...native conditions, the less activation you can achieve using a CRISPRa. Your gene of interest might already...Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon...Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and transcription...

  17. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...Improvements in our discipline for annotation and archiving of data are likely to improve research. What is...common file types? And how long should data be archived? Simplifying data access Some of the above mentioned...data repositories emerge that allow efficient searching and extraction of information. Looking a little...

  18. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ... in your host organism. Because transcription machinery differs between cell types or organisms, promoters...from beta actin gene Constitutive  Ubiquitous. Chicken version is commonly used in promoter hybrids. ... promoter Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice...

  19. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... lab. Some are rabbit immunoglobulin G1 (IgG1) chimeric antibodies, and others are rat or mouse IgG2a....reproducible rat or mouse rAbs. The other eight are chimeric antibodies composed of rat or mouse variable regions...antibodies comes from IPI’s work with the Boston Children’s Hospital and Harvard-based lab of Tim Springer...

  20. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...getting dark and wintry with the end of year approaching. We’re always looking for something to brighten...bright fluorescent proteins with different thermal quenching rates (mNeonGreen and tdTomato), they produce ...demonstrates that taking advantage of fluorescence quenching properties is a promising strategy for developing...
Showing: 181 - 200 of 569 results