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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 17 results for: CHI;

Showing: 1 - 17 of 17 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...gently touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well...nothing falls into the plate. Leave dilutions on the chilled 96-well freezer block in the BSC until ready to...Prepare the Master Mix Place a ddPCR plate onto a chilled 96-well freezer block and set aside in the droplet...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 20 µL of the master ...aspirating the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. In this protocol...

  2. CRISPR Library Amplification

    Type
    Protocol
    ...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to ...Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent...ice for 15-20 minutes or until completely thawed. Chill a box of 200 µL micropipette tips in a -20 °C freezer...kV, 1 pulse. Aliquot 25 µL DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation involves...sterile LB at 4 ℃. Day 2 (morning) Before beginning, prechill at least four conical tubes on ice and ensure ...

  3. Gibson Assembly Protocol

    Type
    Protocol
    ... order primers that are 60 bp long, with 30 bp matching the end of the adjacent fragment and 30 bp annealing...the assembled parts. Tips and Troubleshooting “Stitching” fragments together using oligos When you need...Pro-Tip Please note that the way to design the “stitching” primers and the amounts of primers to include...TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA...Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...

  4. Lentivirus ddPCR Titration

    Type
    Protocol
    ... gently touching the screen. Preparation of the Master Mix Place a ddPCR plate onto a chilled 96-well ...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 16 µL of the master ...pipetting the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the...

  5. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below the primer...liquid to the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...minutes at 95°C in a PCR machine or in a beaker of boiling water. If using a PCR machine, incubate the sample... that does not contain antibiotics. h. Without touching the sides of the dish, gently add DNA:FuGENE® ...PubMed . Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Zufferey R et...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...surface and should be handled carefully. Avoid touching the cells when replacing media. T-175 flask, Corning...plasmids, we can determine the total μg/bp we need to achieve a 1:1:1 molar ratio of each plasmid: 2000 μg / ...

  9. DNA Quantification

    Type
    Protocol
    ...Blanking' measures the background inherent to the machine and your solvent. If using a NanoDrop to measure...

  13. Water Bath Protocol

    Type
    Protocol
    ...can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room temperature....

  14. Centrifugation

    Type
    Protocol
    ...separate different components in a liquid sample. It achieves this by using centrifugal force, which is generated...

  15. Pipetting Protocol

    Type
    Protocol
    ...submerge the pipette itself into the liquid and avoid touching the pipette to the inside of the tube. Only the...

  17. Kit Free RNA Extraction

    Type
    Protocol
    ... (Link opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new window) TRIzol...
Showing: 1 - 17 of 17 results