...co-founder of BGI-China presenting, it’s no wonder it was attended by over 300 Chinese scientists eager...Having never been to China myself, I was pleased to be able to participate when the organizers of the...Dmitry Samarsky, and a very welcoming team from China’s Ribobio, the illustrious speaker list attracted...were potential Addgene requestors and depositors. China is in one of the top 10 countries in number of plasmid...where one can shop for components of traditional Chinese medicine. There were hundreds of stalls selling...replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no proteins). The chemistry... china...

...successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though ChIP requires ...Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein...protein:DNA interactions. ChIP works by using antibodies to capture protein:DNA complexes with antibodies...downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for exploring...an approach for the faint of heart!  The Process ChIP can be done for either tissues or cells. The process...step. For this blog, we're going to focus on the ChIP process and not the upsteam sample collection process...process or the downstream analysis process. And ChIP itself begins at...  Crosslinking Once you have your...

...Fc region may be changed.   Chimeric antibodies can help A chimeric antibody is made by changing an... are subtle, chimeric antibodies should be revalidated before use. In addition, chimeric antibodies will... a similar problem, then isotype converted, or chimeric, antibodies may be the right solution for you.... B antibody to a mouse IgG2a Fc. The resulting chimeric antibody would have the original variable regions...isotype (Liddell 2013). In most cases, the resulting chimeric antibody retains the binding specificity to the...utility of recombinant antibody tools, the resulting chimera may have different binding properties (Morelock...isotype-specific secondary antibodies when multiplexing with chimeric antibodies. In the case above you should use an...

...on to work in patent law, business development, teaching, etc. Church also encourages his mentees to follow...

...plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “...

...unrelated worm (C. elegans) [2]. Delivering dsRNA matching a specific mRNA sequence triggers a cascade of... through physical blocking of the translation machinery. In the Sánchez lab protocol, dsRNAs are delivered...

...knockout in immune systems.   CHIME We previously developed CHIME (CHimeric IMmune Editing system) to enable...have not been widely employed in vivo. The CHIME and new X-CHIME systems, developed and deposited at Addgene...hindering studies post embryogenesis. Using C-CHIME and S-CHIME, we discovered that double knockout of PTPN1...Schematic showing the X-CHIME approach. Figure from LaFleur, et al. 2024.   The X-CHIME deposit also includes...preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a...LaFleur et al., 2019a and LaFleur et al., 2019b). CHIME works through transduction of Cas9-expressing (Rosa26...quite useful for interrogating these scenarios. X-CHIME We recently developed and deposited four new systems...

... a scientist, it is useful to make literature searching and reading a regular part of your routine. Set...scientific literature a little bit at a time. Searching for literature My go-to search engines for finding...a network with an institutional subscription), archived on PubMed Central, or in preprint onBioRXIV. One...Mendeley – these programs offer more options for searching and sorting your own digital library as well as...some integration with internet-wide literature searching. Update 5/6/2015:Here's another option that may... of their F1000Workspace.  Give your favorite searching, reading and organization tips in the comments...

...and have coaching plans that are financially accessible. Sometimes only a few hours of coaching time can...Some have online coaching tools that can be very effective and also help keep the coaching costs low. Most...also launched an online career coaching program that might make her coaching more affordable for you. There...making this transition). Another coaching group is Still Point Coaching. One nice thing about a group is...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...exception, that this helps people do a better job at reaching their goals.  Check out Joanne's Reddit AMA I...that few scientists are aware of the benefits of coaching relationships and do not know where to look for...

...engineered DNA-binding molecule-mediated ChIP (enChIP). By using enChIP, specific genomic regions can be easily...technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP are recent examples. We also...locus-specific ChIP). These methods consist of insertional chromatin immunoprecipitation (iChIP) and engineered...the insertional chromatin immunoprecipitation (ChIP) (iChIP) technology, which uses locus-tagging and affinity...requested plasmids for enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene...molecule-mediated chromatin immunoprecipitation (enChIP), both developed in the lab. In June 2014, Dr. ...  Addgene: Your lab has worked extensively with enChIP systems. Can you describe this technology and its...

...the interview with Joachim Goedhart Podcast breakdown Intro Chapter 1 - How Joachim ended up at the University...art. Outro Many thanks to our guest, Joachim Goεdhart! Joachim Goedhart is an assistant professor at...Scientist Benoit Giquel. Ben recently spoke with Joachim Goεdhart, a professor at the University of Amsterdam...University of Amsterdam Chapter 2 - Joachim’s Academic and career goals. Career advice for people going... microscopy. Chpater 4 - Research projects in Joachim’s lab. New microscopy probes and more. Chapter 5...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Special thanks to our sound engineer/producer...Fluorescent Proteins featured topic page Read Joachim's Series on Choosing the B(right)est Fluorescent...

... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence... set of possible downstream states that can be achieved by expression of other recombinases. Multiple ...terminators, etc) are composed. Fear not – the state machine design space is highly degenerate and often times...Roquet et al., Synthetic recombinase-based state machines in living cells. Science. 353, aad8559 (2016)....

...naïve questions. During the panel, an artist was sketching her interpretation of the discussion in markers...

...gene. CHIME can also be used for high throughput screens using pooled libraries. Find the CHIME plasmids...successfully visualized in vivo using ultrasound in Escherichia coli and Salmonella Typhimurium. This work demonstrates...cell lineages in vivo using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen ...the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any...

... (2002): 682-688. PubMed PMID:12404098.  4. Lin, Chi-Wang, Cindy Y. Jao, and Alice Y. Ting. "Genetically... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ...to have occurred that was passed down to the grandchildren; in this study,epigenetic changes were statistically...influenced cardiovascular mortality of the female grandchildren." BMC genetics15.1 (2014): 12. PubMed PMID: ...

...based on the original Tol2kit generated by the Chi-Bin Chien lab (Kwan et al., 2007) and includes four promoters...diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene expression...With eSpCas9(1.1) and coselection, Agudelo et al. achieved editing rates as high as 83% with NHEJ and 40-...shortening leads to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation...

... 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on power is...thanks to our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...to select a photostable fluorescent protein. Photobleaching is the irreversible destruction of a fluorophore...fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins may display reversible...Shaner et al, 2008; Bindels et al, 2017) and photoswitching (Kremers et al, 2009), which usually are undesired...destroyed. Factors that affect photostability The photobleaching rate of a fluorophore primarily depends on ...

...This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai...cells.   Many thanks to our guest bloggers Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe ...Leiwe and Takeshi Imai from Kyushu University. Richi Sakaguchi is a graduate student at Kyoto University. ...25157152. PubMed Central PMCID: PMC4246963. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright...light microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the...Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al., 2018) (Figure 1). Once introduced, fluorescent...you use AAVs, it typically takes a few weeks to achieve optimal expression of XFPs. Any longer and the ...

...resulting in the following hierarchical model: Because the U.S., Europe, and China account for over 75% of...instabilities in the China data, so we recommend interpreting the model results for China with more uncertainty... dominated by the U.S. (42%), Europe (23%), and China (14%). Spikes in plasmid requests correspond to ...encountered COVID-19 at different times. Most notably, China was affected by COVID-19 earlier than the rest of...COVID-19 event windows as 01/15/20 -- 03/15/20 for China and 03/11/20 -- 05/19/20 (the last date in our dataset...day across regions, with Europe most impacted and China least impacted. The inferred weekend effect is, ...well. However, we observed one complication in the China data – there is a period of 1 month, from 1/22/20...