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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 659 results for: Clu

Showing: 181 - 200 of 659 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...the ATCC Declutter! Keep the hood clean Hoods should not be used as storage cabinets. Clutter not only...ll be brought under the hood with 70% ethanol, including your glove-covered hands! You should additionally...

  2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... transferable into multiple bacterial species including several involved in human disease. However, the...every ~100 to 1,000 colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer...Mobile-CRISPRi expands CRISPRi to non-model bacteria including pathogenic strains and environmental isolates....

  3. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...NgAgo optimization before we can make any major conclusions: That said, many people are having difficulty...Some great places to get your results out there include: Google Groups - I've specifically set up a forum...Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing methods in brain cells...

  5. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...be relied on exclusively because historical studies of pathogen research have conclusively shown that barrier...For example, an sgRNA-only drive will spread exclusively through populations that already express Cas9...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use...course multicistronic vectors do not have to exclusively be used as a means of detection; they are useful...note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized 2A and...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...terrific getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs...regions” filter unchecked so that these regions are included in the search to maximize the alignment length...sequence alignments that BLAST is unable to handle, Clustal is our frequent choice for pairwise or multiple...

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...current genome engineering applications of CRISPR including: generating single or multi-gene knockouts in ...) that harbors two surface exposed cysteines, including the native C547, allowing for ligation to pyridyl...Senior Scientist at Addgene.  Andrew's interests include genome engineering and microbiology.     References...

  9. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ... Selecting crispr in Guides will return links including our CRISPR guide.  For a closer look at this new... deposit prior to publication and were able to include specific plasmid ID numbers. If you know the ID...suggest direct links to other types of searches including depositing labs and institutions. For example,...

  10. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ... different.  Conclusion Figure 7: Mean inferred effects by region. In conclusion, our model infers...requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 years for 35 papers...

  11. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...addressed in a somewhat cursory manner, their inclusion here adds much needed context to understand every...state of science would be complete without the inclusion of some interesting case studies, and Zimmer provides...who originally isolated the GFP gene but was not included amongst the Nobel prize winners for developing...

  12. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...mind that access and availability to resources (including the internet!) can vary depending on factors like...your best to understand what kind of resources - including your time - will be required to produce an outcome...naked eye or to make things more accessible by including, highlighting, or omitting details. Digital or...

  13. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce...CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: lentiCRISPR... observed in small trials for other diseases, including the common hematopoietic disorders sickle cell...

  14. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... and management process or the whole process, including grant readiness, funding opportunity research,...grant training, mentoring and coaching services, including Smartegrants, to help those entering the Grants...LLC, a grant professional development firm that includes the social media community GrantChat. She has ...

  15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...scientists employ GFP for a wide number of functions, including: tagging genes for elucidating their expression...detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations and pH, using...selection: Expression constructs like plasmids often include GFP as a marker to help identify which cells have...

  16. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ...(GTA). The GTA has a wealth of information and includes annotated images of the brain sections describing...enhancers and their neuroscience research. This includes other data, tools, and resources. Luckily for ...are also more resources available at Addgene, including the Allen Institute for Cell Science Plasmid Collection...

  17. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools allow the researcher to...again, this post has a lot of acronyms, so we've included a glossary at the end of this post! Figure...for their application from among many options.  Conclusion With these advances, every base can now be directly...

  18. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries...viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically...aliquot to full use! Common conditions to optimize include incubation time, antibiotic conditions, and amount...

  19. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...previously not associated with ricin regulation, including almost all genes involved in the production of...non-targeting guides and 6,750 safe-targeting guides were included in the library. Safe-targeting guides were designed...activity, Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting...

  20. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance ...can be used for an array of detectable proteins including fluorescent proteins, antibiotic resistance, and...
Showing: 181 - 200 of 659 results