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Showing: 181 - 200 of 513 results

  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic... thawed more than once. Fibronectin is another membrane-interacting protein that can be used to enhance...receptor-independent virus adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. ...

  2. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...designing primers, and running analyses. New team members have taken up to a month to design a single 96-...began building CrispyCrunch in July 2018 and in November, we began using it internally. Guiding principles...biopython, bwa, samtools, bowtie, UCSC genome browser, Ensembl, and others. It builds on existing popular web ...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...it may benefit you to work as a team with other members of your lab, a lot of the “soft skills” required... In an academic environment, surrounded by lab members who are all working on related topics, scientific...will be part of a team in industry with most team members giving oral presentations, you will also have to...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...In cell-free expression systems, proteins are assembled in vitro using purified components of the transcription...Cell-free expression systems are ideal for fast assembly of more than one protein in one reaction. A major...advantage of these systems system is their ability to assemble proteins with labelled or modified amino acids...

  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...VP2C fusion protein anchors to VP1, which self-assembles into a compartment encapsulating the protein of...contains a series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs. zCas9i is a high efficiency...2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane tethered GFP to enhance axonal labeling and synaptophysin-tagged...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...Jorgensen Lab recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR genomic...SapTrap offers scientists a convenient one tube assembly reaction to generate high-throughput targeting...templates. A final ligation reaction correctly assembles the final targeting vector (Fig. 1, Step 4), and...

  7. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...always a good idea to introduce yourself to a few members of your desired department during the application... Different platforms have different quirks, so remember to practice on the program you’ll be using for... be difficult. However, an online meeting with members of your desired program will allow for you to have...

  8. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive...regions by default, meaning that you do not have to remember to adjust any blastp algorithm parameters. We ...http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http://www.ebi.ac.uk/services http://www....

  9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...figure above), which generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm...nucleofection, which forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques...protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated methods, while...

  10. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...notebooks and computer files. If you asked your team members or colleagues: How much unpublished AAV data do...Therapy - Methods and Clinical Development, 18(September), 98–118. https://doi.org/10.1016/j.omtm.2020.05.018...Stimulation. Frontiers in Human Neuroscience, 14(December), 1–12. https://doi.org/10.3389/fnhum.2020.578687...

  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...compromising the photon budget. Likewise, the switch from ensemble imaging to single-molecule imaging places a greater...recently discovered that incorporation of four-membered azetidine rings could substantially enhance the...analogs without sacrificing their small size and membrane permeability (Figure 2c). These cell-permeable...

  13. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...data from the ENCODE project, find enhancers in embryonic stem cells, and conclude with a targeted CRISPR...for NANOG, an essential transcription factor in embryonic stem cells (ESCs). Your ChIP-seq finds just over...CRISPR screen. We start by infecting millions of embryonic stem cells (ESCs) with a lentiviral vector, an...

  14. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...development, complex genetic programs specify and assemble diverse arrays of neurons, forming the neuronal...subgenome-scale CRISPRa screening approach in embryonic stem (ES) cells to determine which transcription...gene-function relationships in the CNS using CRISPRa as a member of the Gelbard Laboratory at URMC.     References...

  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... The G·U wobble base pair. EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001 Additional resources...mammalian cells. This was the first codon optimized membrane protein expressed in mammalian cells. In this ...

  16. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...for attendees and helping societies help their members get the most out of their meetings. Amanda Gunn...Gunn ran randomized coffee trials for us at ASCB|EMBO 2019, matching Mentors with Singletons for short coffee... a culture that brings more value to ALL their members.  Shoutout to our mentors and supporters Thanks...

  17. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...structures in RNA transcripts Effects on transcription (remember, it’s not all about translation - e.g. codon choice... isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157...transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366. PubMed PMID: 2265611...

  18. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ... streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher linkers and ...and target capture module (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP nucleosomes...loss. Ci Ji Lim’s lab found that adding Late Embryogenesis Abundant (LEA) proteins, small proteins that...

  19. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non toxic to cells. Because it rapidly...split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of a “Top” and ...fluorescent split aptamer for visualizing RNA–RNA assembly in vivo." ACS synthetic biology 6.9 (2017): 1710...

  20. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...heads and long legs that protrude from the cell membrane, physically connecting the extracellular environment...the integrin, where it is resisted by the ligand embedded in the extracellular environment. This further...the extended open integrin conformation. During embryonic development, extended-open integrins enable tissue...
Showing: 181 - 200 of 513 results