Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 383 results for: FLU

Showing: 181 - 200 of 383 results

  1. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...bodies decorated with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed... body dynamics. Second, we showed that these fluorescent R bodies can rupture E. coli spheroplasts (cells...

  2. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...application. Each visual barcode consists of a fluorescent protein coupled to a specific subcellular localization...different localization peptides with four different fluorescent proteins, and inserting each visual barcode into...and reporters that drive the expression of the fluorescent protein by a specific transcription response ...

  3. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...protein. OptoNBs were also tagged with an infrared fluorescent protein tag which let the team detect its co-...revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead, suggesting that OptoNBs...OptoNB-SOS activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These ...

  5. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...number in a cell. One can attempt to eliminate fluctuations due to copy number by introducing genes of interest...output. The input, plasmid copy number, directly influences gene expression. By itself, increasing the copy...stabilized promoters perform during these types of fluctuations. When cells are dividing rapidly, multiple rounds...

  6. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ... external factors can have a quite noticeable influence on the outcome and cause variability in the testing...and is not always recommended.  Environmental influences like bright light or noise should be avoided ...ultrasonic frequencies (> 20 kHz, produced from fluorescent light bulbs, for example) that are outside the...

  7. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...should appear fluorescent if they were infected. While there may be some low-level fluorescent protein or...virus. Alternatively, if the virus encodes a fluorescent protein, the packaging cells could be examined...

  8. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...is particularly useful when vectors carrying fluorescent markers are used as it eliminates the need for...for antibody staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes... lentivirus Volume and transduction time will influence lentiviral titers as will the target cell type...

  9. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...co-express your gene along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow...co-express your gene of interest along with a fluorescent protein or selectable marker, it is easiest to...genes at equivalent levels upstream of an IRES fluorescent reporter for easy detection.5  Just released:...

  10. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...Zimmer, whose previous books focused primarily on fluorescent proteins and their applications, tackles much...middle-aged scientists, the identification of Green Fluorescent Protein (GFP) and its development into an essential...reading this review--in the current period of rapid flux, the chapter might still feel slightly dated by ...

  11. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...are easy to trace and not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color...constitutively express a red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral...morphology studies when used as part of a two part fluorescent tag-then-express system that allows for controlled...

  12. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but for simplicity we ...antigen in the sample. A variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available...read on a standard plate reader as opposed to chemifluorescent, and chemiluminescent methods that require...

  13. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...slow-growing protein fiber in the cytoplasm and can be fluorescently labeled. Using lentiviral vectors to stably ...different promoters, specific time points appear as fluorescent bands in the fiber, resembling a “ticker tape... treated with a phorbol ester activator gave fluorescent signal in the ticker tape from cFos::GFP-iPAK4...

  14. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used...without signs of toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin...plasmid and 954 ng CMV-gvpNJKFGWV plasmid into 70%-confluent well of HEK cells in a 6-well dish and change ...

  15. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...successfully and expressing the protein. For example, fluorescence microscopy images resulting from our experiment...was not successful due to the absence of GFP fluorescence from the living, viable cells. This result presents...produces the expected effect, which is green fluorescent cells (Figure 3).   Figure 3: Expression of Plasmid...

  16. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...purify a variety of peptides including antibiotic efflux pumps and ankyrin seven-repeat proteins: Sjuts...Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

  17. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...laser impacts the magnitude of FSC, SSC, and all fluorescent stains. The cytometer's software will allow you... my analysis. For this measurement, I used a fluorescent dye that can only enter dead cells, while living...interested in measuring a population based on a fluorescent stain, you might be wondering which second measurement...

  18. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ... cells were labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed...through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely with target...use in western blot, immunoprecipitation, immunofluorescence and flow cytometry. F1000Research, 13, 817...

  20. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling...agar art have a look at our Microbiology and Fluorescent Protein Resources and get started!  I learned...
Showing: 181 - 200 of 383 results