We narrowed to 192 results for: GFP
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TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry fluorescence...involves tracking the ratio of mCherry-positive to eGFP positive cells through fluorescence microscopy or...
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With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m.... including those targeting EGFP, BRAF, and MAP3K4. The gRNAs targeting EGFP can be used as positive controls... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m....nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features, such as Cas9, weren... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m.... pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1, and pAdSh.U6.gRNAGFP. Gonçalves says that advantages of AdVs include...guide RNA constructs, pAdSh.U6.gRNAS1 and pAdSh.U6.gRNAGFP, and also Adeno Cas9 and Adeno EA. Or if you're... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....relating to that specific protein (for instance: EGFP). Users can “favorite” certain proteins, or create... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m.... QY (EC*QY, sometimes normalized to the value of EGFP). The higher the number, the higher the theoretical... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and 2) gRNAs Number of loci targeted One ... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....Multicistronic Element Expression Type MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start and gene... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m....commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use the drop-down menu to ... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....