We narrowed to 332 results for: NUC

...high-throughput screen for molecules affecting alpha-synuclein oligomerisation. Eastwood et al. FEBS Lett. 2017...mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant J. 2010 Yuhai Cui Red mScarlet...

...an unmethylated CpG to behave naturally in the nuclear environment. One good system to balance these needs...CRISPR101: Editing the Epigenome  Mapping the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read...

...lab and Feng Zheng’s lab had already developed nucleic acid detections tools based on Cas12a and Cas13...one-step RT-qPCR reaction to detect SARS-CoV-2 nucleic acids. Mammalian targets During the 2003 SARS outbreak...

...Assembly: a) Graphic depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of...libraries encode target sites flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM...

... than the more efficient single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR...codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids...

... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the...expression using an engineered CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093...

... "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737. PubMed ... a platform for 3′ UTR biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed...

...overhangs with the same three nucleotides in a row; (4) no more than two nucleotides in the same position; (...

...administration. However, until recently safe delivery of nucleic acid cargo (DNA or RNA) to target cells has been...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

...mutations, frame shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements... reference; and identifying mixed plasmid and nucleotide populations. All of these topics require additional...

...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess...

...cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell, 2008. 132(4): p. 567-82. PubMed... S. and D. Wolf, Totipotency, pluripotency and nuclear reprogramming. Adv Biochem Eng Biotechnol, 2009...

...ends. These fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out ...When mixed, topoisomerase I provides both the endonuclease and ligase function while 5’ adenosine overhangs...

...including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs ...power of this system lies in the ability of the endonuclease Cas9 to create a DNA double stranded break (...

...constructs for visualizing cellular components (nucleus, endosomes, lysosome, golgi, ER, inhibitory synapses...TnpB (A) and Cas9 (B) genome editors. Effector nucleases are shown bound to the target DNA, recruited by...

...endA+. endA encodes a thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid...recommend performing an extra wash step to remove endonucleases (see figure at right for an example). Look out...

...non-specific endonuclease (Cas9) and a single stranded guide RNA (gRNA). The ~20 nucleotide targeting sequence...

...VchINT does not recruit other nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms...

...innate immune system; and reconfiguring of the nuclear compartment, gene expression, and protein translation...Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https...

..., W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the Bryan strain of Rous sarcoma virus:...purification of the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences...