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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 329 results for: NUC

Showing: 181 - 200 of 329 results

  2. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737. PubMed ... a platform for 3′ UTR biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed...

  3. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...high-throughput screen for molecules affecting alpha-synuclein oligomerisation. Eastwood T, Baker K, Brooker ...mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant J. 2010 Yuhai Cui Red mScarlet...

  5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...lab and Feng Zheng’s lab had already developed nucleic acid detections tools based on Cas12a and Cas13...one-step RT-qPCR reaction to detect SARS-CoV-2 nucleic acids. Mammalian targets During the 2003 SARS outbreak...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...Assembly: a) Graphic depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of...libraries encode target sites flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess...

  9. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...mutations, frame shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements... reference; and identifying mixed plasmid and nucleotide populations. All of these topics require additional...

  10. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...ends. These fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out ...When mixed, topoisomerase I provides both the endonuclease and ligase function while 5’ adenosine overhangs...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs ...power of this system lies in the ability of the endonuclease Cas9 to create a DNA double stranded break (...

  13. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...endA+. endA encodes a thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid...recommend performing an extra wash step to remove endonucleases (see figure at right for an example). Look out...

  14. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...non-specific endonuclease (Cas9) and a single stranded guide RNA (gRNA). The ~20 nucleotide targeting sequence...

  16. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...innate immune system; and reconfiguring of the nuclear compartment, gene expression, and protein translation...Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ..., W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the Bryan strain of Rous sarcoma virus:...purification of the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences...

  18. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

  19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins in 2004. In addition,...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress...
Showing: 181 - 200 of 329 results