We narrowed to 457 results for: PAC
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TypeBlog PostPublishedOct. 3, 2024, 1:15 p.m....repository! This includes the first Addgene prep packaged in the AAV9-X1.1 serotype. In rodents, AAV9-...You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1! We also have eight new AAV-PHP....
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An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....first week at Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity...safety, general work skills — such as using Google workspace — and got the chance to work on my very own research... -
We're Updating Our Hold for Publication Policy
TypeBlog PostPublishedJune 24, 2025, 1:15 p.m....add the citation and release the hold. Increasing impact Making the plasmids available as soon as your paper...paper is published helps maximize the potential impact of your work. Plus, it’s costly for us to process... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....experiment and the organism you are working in. The spacing of genes and whether or not it’s ok to disrupt ...the known and unknown microbial coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m....aliquots in a kit with a huge amount of wasteful packaging. Turns out, one could make a lifetime supply ...indefinitely at room temperature (thus also saving freezer space). The procedure becomes less attractive when noting... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m....blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m....Developmental biology caught me early on: a simple egg is packed with information. I am fascinated with how developmental... from. Put your skills and interests into your backpack, and step out there into the galaxy. TL;DR: Many... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m.... increasing the impact of the work, and as a collateral benefit, increasing the impact of the specific... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....pots, trays, soil, fertilizer, seeds and a growth space with fluorescent lights. Other experiments require... answer questions and provide support. Growing capacity - ABRC outreach today Over the years, the outreach... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....ColE1 A Relaxed pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent...phage-derived ori that allows for the replication and packaging of ssDNA into phage particles. Plasmids with phage-derived... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....time. “At an early age, I was fascinated by the impact that medicines can have on human health. As a teenager...organizations are less common, they can be very impactful. At Novartis, Stegmeier deposited the ClonTracer... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....engage their readers or listeners thus expanding the impact and appreciation for their research. Long story...judgement. Eventually, we published the paper in a high-impact journal and it worked out for all of us.” This ... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m....for in vivo gene delivery, but have limited packaging capacity making them difficult to use with CRISPR ... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....plasmids and new cloning vectors such as pBR322, pACYC, and pUC were developed to provide higher copy number... discovery in the 1950s, plasmids have greatly impacted many areas of biological research and have been... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....tech companies using only laptops and some server space. But what if you want to be an entrepreneur in ...you start at a disadvantage in the health science space if you don’t have an advanced degree behind your... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....to a protein of interest thereby giving it the capacity to enter cells. One such CPP was discovered in...directly with proteins removes the limitations of packaging size associated with viral vectors. For these ... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....can be limited by the need for a specific PAM (protospacer adjacent motif) in order to bind the target sites...These loops not only provide a 3-D structure for packing genomic DNA into nuclei, but are also thought to... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....menstrual pads while we talked about the environmental impact of disposable products. A great deal of scientific...Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived climate... -
DAP arrays for multiplex gene editing
TypeBlog PostPublishedJuly 7, 2022, 1:15 p.m....promoters, has limited the number of gRNAs that can be packaged into viral vectors for potential in vivo applications...therapeutic potential of the DAP array, the Gao Lab packaged MBE and MPE elements for deliveries via adeno-... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....and novelty in scientific research and in any workspace. Re-design lab meetings with a focus on discussion...making lab meetings more interactive and creating space for discussion at the end of each talk - time for...