We narrowed to 193 results for: SPL
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TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m....digest products of the unmodified plasmid will be split into two smaller bands (Figure B, digest A). In ...
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Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....state. In contrast, Brainbow-3.1 and -3.2 do not display default fluorescence due to the addition of translation-blocking... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m....previously made mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....choose are teaching the stuff you want to know and display character traits and skills that you hope to grow... -
Antibodies 101: Validation
TypeBlog PostPublishedMarch 24, 2022, 1:15 p.m....alternative translation start sites or alternative splicing that could lead to some form of the target protein... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m.... Types of transposons Transposable elements are split into two classes based on their mechanism of transposition... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....slowly gone crazy. As it was, my partner Mike and I split the work, shared the credit, and we both won accolades... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m....D.A., et al., Generation, purification and transplantation of photoreceptors derived from human induced... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog PostPublishedDec. 20, 2017, 2:33 p.m....critical role the work would not have been possible. Display of work in artistic exhibitions or showcases (not... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....when transiently overexpressed in CHO cells and displayed similar labeling patterns on different primary... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m.... to tumor formation in iPSC-derived and iPSC-transplanted mice. Cre-deletable or Tet-inducible lentiviruses... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon...cassette. The simpler system of pCRISPomyces-2 displays a higher editing efficiency, perhaps due to its... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m..... Each gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the...