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TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m....replication experiment that you conducted as part of setting up your project. These data are valuable but remain...
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New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....be useful to you.) With these goals in mind, we set out to update our web resources and plasmid pages... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog PostPublishedJune 23, 2020, 1:15 p.m....available to scientists working in a for-profit setting. In light of the need for basic research during... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m.... to complement our next-generation sequencing, setting new standards for plasmid quality control. Through... -
Build Your CRISPR Vocabulary
TypeBlog PostPublishedJuly 2, 2024, 1:15 p.m....target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....Microbiology Page! The start of the conference After setting up the Addgene booth on Thursday evening, I stayed... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....in writing your own blog post about a plasmid or set of plasmids you’ve deposited with us. Don't forget... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog PostPublishedNov. 17, 2015, 3:30 p.m....Freshers Fair, all of the societies within a university set up stalls or stands on campus and recruit students...network, find groups with common interests, and settle into university life. The fairs are also an opportunity... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m...., cells may be imaged under lower illumination settings, lowering photobleaching and phototoxicity concerns... -
9 Tips to Achieve Success in Academia
TypeBlog PostPublishedApril 1, 2014, 5:33 p.m.... 1. Keep up with the literature. He recommends setting up a few Pubmed email alerts for new papers with... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m.... the transparency window of mammalian tissues is set by the properties of hemoglobin and melanin pigments... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m....” Wang’s library is designed to target a smaller set of genes and it includes more guides per gene. The... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....sites in combination with existing ChIP-seq data sets, of which there are many readily available. Based... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....relative to mCerulean imaged with a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine and... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....serological pipettes from Corning®, Falcon®, or Axygen®, set up a bin or box in your tissue culture room for the...but your institute may still be interested as it offsets the cost of regular waste services. Learn about... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....scientists can purify and study it in a controlled setting. Read more: Plasmids 101: Protein Tags Produce... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....Bertuzzi welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....technology that can be used to quickly barcode a set of DNA sequences. In this post, Alan Wong (formerly... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....simplify my work with an Arabidopsis mutant that can’t set seeds. I study the development of the mutant plants... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....you could see yourself taking? If so, consider setting up an informational interview with them to learn...