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We narrowed to 608 results for: Sis

Showing: 181 - 200 of 608 results

  1. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...of a senior thesis), I knew early on that I wanted to work with a partner. WPI’s emphasis on teamwork ...decision about something? Fix a problem? Test a hypothesis? Whether you officially call it a “project team... risky shots at the goal rather than going for assists that are more likely to lead to scores. That player...

  2. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...post was contributed by Jordan Ward who is an assistant professor at the University of California, Santa...elegans relied on selective markers such as drug resistance (Chen et al., 2013), fluorescence (GFP; Tzur ...Thank you to our guest blogger! Jordan Ward is an assistant professor at the University of California, Santa...on gene regulation in nematode molting and spermatogenesis. His group also builds tools to improve genome...

  3. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    .... Like other ABEs, the editor they were using consisted of Cas9 fused to an engineered adenosine deaminase...tool for predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun...A., Yang, D., Mok, B., Replogle, J. M., Xu, A., Sisley, T. A., Weissman, J. S., Adamson, B., & Liu, D....developed using CRISPRi screens, target-library analysis, and machine learning. Nature Biotechnology, 39...

  4. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...a prestigious institution: This criterion is consistently under scrutiny. USCIS wants you to prove that... is very important in this case.  You can still assist your PI in the peer review process, but remember...love of science and technology, Rachel is proud to assist companies and foreign nationals working in the ...dedicated to the practice of US immigration law and assists individuals, researchers, entrepreneurs, companies...

  5. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...optimize and troubleshoot the system. Site Directed Mutagenesis by PCR & Single Base Editing with CRISPR For ...technology may make some uses of Site Directed Mutagenesis by PCR obsolete! Honorable mentions Of course... in chronological order here: Site Directed Mutagenesis by PCR Google Forums Round Up: First Impressions...

  6. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...this article: “A systematic identification and analysis of scientists on Twitter.” This paper addresses..., or are friends, are included in this cluster analysis. The closer two dots are to each other, the more... C. R. (2017). A systematic identification and analysis of scientists on Twitter. Plos One,12(4). doi:...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ... than point modifications. CRISPR base editing persists through multiple cell divisions, indicating that...specificity. In this first, the Liu lab used phage-assisted evolution selection systems to generate ABE8e(... a similar approach. Dali Li’s lab’s A&C-BEmax consists of a fusion of both cytidine and adenosine deaminases... E., Doudna, J. A., & Liu, D. R. (2020). Phage-assisted evolution of an adenine base editor with improved...

  8. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...dopamine. Methods for measuring Dopamine In vivo microdialysis The gold standard method of measuring DA and...neurotransmitters in the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011...References Darvesh, Altaf S., et al. "In vivo brain microdialysis: advances in neuropsychopharmacology and drug...

  9. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ... these techniques and is a multi-step process consisting of digestion, purification, ligation, and transformation... 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for...Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your Plasmid Background with...

  10. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...social and regulatory reasons. 1.) The transposon consists of a mirrored set of inverted repeats (green) ...1) an SB transposase, the enzyme required for catalysis of transposition; and 2) a transposon containing...determined by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In their Nature...

  11. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...culture, only the plasmid with the antibiotic resistance gene and origin will replicate, and the transgene...recombined vector backbone contains the antibiotic resistance gene (AmpR) and origins of replication (ori and...of bacterial plasmid DNA: electron microscopic analysis of in vitro intramolecular recombination. Proceedings...

  12. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...labels cells with heritable barcodes. Barcodes consist of an 8bp variable region and a 6 bp fixed region... a sample of cells was collected for scRNA-seq analysis and the remaining cells were maintained in culture...detailed CellTag protocol on protocols.io, code and analysis tutorials on GitHub, and a CellTag lineage data...

  13. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...protein localized to their expected locations. An analysis of mWasabi gave similar results. We hope this ...

  14. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...replication and gene transcription during vertebrate embryogenesis. My interests, though, have proven unruly. Instead... at Science magazine. That one I just could not resist applying for. Not that I knew anything about being...like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance of copy...

  15. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage When I...Ribonucleoprotein (RNP) Delivery The RNP delivery method consistently leads to high on-target editing and low off-...strategies activate different HDR sub-pathways like synthesis-dependent strand annealing (SDSA) and single-strand...

  16. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...GST-glutathione. Biotin-based immobilization for kinetic analysis Proteolytic removal of the fusion protein could...could provide a more relevant kinetic analysis, but the trick is to utilize a second immobilization method... purification strategy outlined here provides a basis to determine binding kinetics using a multitude ...

  17. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ... Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion ...approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase. Shuman S. J ...

  18. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the primer assist with restriction...selecting restriction sites, you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer, to ...

  19. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... often describe a process that begins with cell lysis to release AAV particles with subsequent purification...glioblastoma). They also found that exo-AAVs were more resistant to neutralizing anti-AAV antibodies. This is particularly...areas that are difficult to reach, and are more resistant to inactivation through anti-AAV antibodies. ...

  20. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... way into high school classrooms, like gel electrophoresis to separate DNA or transforming cells with ...practice with practical lab skills like developing a hypothesis, precisions pipetting, and good record keeping...in functional genomics research Learn how the Arabidopsis research center brings plant science to life ...
Showing: 181 - 200 of 608 results