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Showing: 181 - 200 of 482 results

  1. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...we are cloning an ORF, we want to clone from the start codon (ATG) to the stop codon (TGA, in this example...reverse complement to get PCR amplification. We can start similarly, taking the final 18 bases of the ORF,...

  2. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...the 3rd edition has now been released), and have started providing recorded interviews with Addgene community...give researchers the background they need to get started using plasmids and the revolutionary CRISPR technology...

  3. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...Hendrik De Buhr (top right) and his colleagues at the Wistar Institute in Philadelphia, PA will put their award...cancers. Joshua Wang (bottom right), also of the Wistar Institute, says that the lab got interested in ...

  4. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    .... “I hope by depositing at Addgene, people will start using them,” Merkx said. “For us, it is one of the...developed something useful [enough] that other people start using it. For us, it’s like a test.” References...

  5. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...libraries from small amounts of DNA i.e 100 pg. At the start of the workshop, NEB gave a brief presentation describing...hoped, we found that regardless of how much DNA we started the process with, we got great amplification of...

  7. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...replication. While we and our collaborators are starting these experiments, there's so much to do that ... to fight the new Coronavirus. To help get you started, here are a few key details. Our set of constructs...

  8. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ... a formal connection, you can just reach out to start the conversation. Many researchers are open to sharing...a really good time! If it sounds right for you, start thinking outside of your current research box, send...

  9. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...about biology in high school, it wasn’t until I started working in my first molecular biology lab in college... 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...

  10. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...the end of your PhD or contract, you should be starting to send out polite emails with your CV to people...Achieve Success in Academia  Advice for Scientists Starting a Lab   ...

  13. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...in statistics and programming, you’d be wise to start working on these skills. Neuroscientists use lots...always suggest a plasmid to us, request that we start making the AAV, or help us produce new educational...

  14. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...allow researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows...different types of research questions. Are you ready to start EvolvR-ing your targeted mutagenesis experiments...

  15. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... staff receives – in fact, the blog originally started as a way to easily store and share in-depth answers...someone new to antibodies might need to successfully start using them at the bench, along with questions we...

  17. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...your actual samples. They may also be used as a starting point for compensation (Fig. 1B-C). However, it... The single color controls should be used as a starting point, to preserve your sample, and you should...

  18. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...That’s a lot of helpful information! To get you started, we’ll give a quick overview of each CasPEDIA feature...the previous information may be all you need to start your targeting experiment. However, many situations...

  19. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...it pairs with its variable chain domain. B cells start by co-expressing IgM and IgD antibodies, but later...recombination of the antibody gene locus. B cells start by co-expressing IgM and IgD isotypes antibodies...

  20. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...manager,” you’re not alone. A few years ago when I started my career transition from bench science to software...solutions with the users, gather more feedback, and start the cycle over again to refine the solution, or ...
Showing: 181 - 200 of 482 results