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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 542 results for: TIL

Showing: 181 - 200 of 542 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...protein:DNA complexes will remain bound to each other until you are ready to separate them. Most proteins are...This will keep the protein:DNA complexes together until you want to break them. Crosslinking is necessary...

  2. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...PRESTO-TANGO: The ligands that activate many GPCRs are still unknown. To identify some of these unknown ligands...adrenoceptor. GPCRs in chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers...

  5. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic... through the above steps, you may find that you still have a number of options. If this is the case, you...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...improvements Miniature CRISPR-Cas systems By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...The titer is tested following each immunization until the desired level of antibodies is reached - typically...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...

  9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...-36 receptor inhibitors in development, there’s still a lot of mysteries surrounding IL-36 biology. “Little...To study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors...

  10. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...environment” with a strong focus on publishing, but still made it so she could “get exposure to the drug discovery...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

  11. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...administration information. Carefully read over all parts until all aspects are understood. While reading the funding...research project grant application" by the NIH Still want more information on the application process...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...in neuroscience, this architecture is frequently still an open question. The connections between neurons...new neurons came from nearby neurons. It wasn’t until later time points that longer-range connections ...

  14. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...large tags into a precise location in the genome is still inefficient, particularly in human cell lines. Furthermore...large regions of homology enable us to effectively utilize the host cell’s inherent homology directed repair...

  15. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...include embryonic lethality, a short lifespan, or infertility and can arise due to copy number, expression ...would get both mutations onto the same chromosome. Until crossing over occurs between the two loci, F2 and...

  16. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...demonstrating low sensitivity to ambient light, and versatile as it can be activated by both low and high-intensity...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...to both people and the tools we use. Scientists utilize an array of tools that rely on DNA amplification...reference is given, de novo assembly in Geneious can still provide additional insights to resolve the assembly...

  18. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...

  19. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... that is useful, but not 100% complete, we will still make this data available as a partial sequence with...Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, ...

  20. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...gRNA complex binds the target DNA without cleavage until it's activated by light. Once activated, Cas9 cleavage...
Showing: 181 - 200 of 542 results