We narrowed to 546 results for: TIL;

...protein:DNA complexes will remain bound to each other until you are ready to separate them. Most proteins are...This will keep the protein:DNA complexes together until you want to break them. Crosslinking is necessary...

... networks of specific proteins in living cells. Until recently, the enzymes most frequently used for PL...paper, they describe TurboID and miniTurbo, which utilize non-toxic biotin, but label nearly as quickly as...

...science, but also science writing.  “And since I was still in grad school, I had the opportunity to take a ... Susan personally found this easier to do while still in school since opportunities, such as writing for...

... additional chemicals or modifications. This versatility opens up the potential for spatial and temporal...are more evenly controlled. The Grefen Lab has utilized the 2in1 cloning system to generate a series of...

...-36 receptor inhibitors in development, there’s still a lot of mysteries surrounding IL-36 biology. “Little...To study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors...

...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic... through the above steps, you may find that you still have a number of options. If this is the case, you...

...improvements Miniature CRISPR-Cas systems By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus...

...all of us played with LEGO as a child (some of us still do), so we are used to the idea of general interfaces...of Click Biology is made possible through the utilization and development of genetically engineered components...

...The titer is tested following each immunization until the desired level of antibodies is reached - typically...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...

...immunohistochemistry techniques. Thus, these proteins provide a versatile method to identify the expression of transgenes...the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small’ tags to identify proteins...

...environment” with a strong focus on publishing, but still made it so she could “get exposure to the drug discovery...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

...administration information. Carefully read over all parts until all aspects are understood. While reading the funding...research project grant application" by the NIH Still want more information on the application process...

...in neuroscience, this architecture is frequently still an open question. The connections between neurons...new neurons came from nearby neurons. It wasn’t until later time points that longer-range connections ...

...is a modified version of L440. This vector is versatile in that it can be used for both in situ hybridization...feedings. Some of the phenotypes are not evident until after amputation. For example, the dramatic “two-headed...

...large tags into a precise location in the genome is still inefficient, particularly in human cell lines. Furthermore...large regions of homology enable us to effectively utilize the host cell’s inherent homology directed repair...

...include embryonic lethality, a short lifespan, or infertility and can arise due to copy number, expression ...would get both mutations onto the same chromosome. Until crossing over occurs between the two loci, F2 and...

...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...

...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...demonstrating low sensitivity to ambient light, and versatile as it can be activated by both low and high-intensity...

...proteins. Discovery of anti-CRISPR proteins That is, until the discovery of anti-CRISPR proteins in 2012 (Bondy-Denomy... RNPs, AcrIIA4 reduced off-target editing while still allowing on-target editing (Shin et al., 2017). ...

...make sure yours counts. Dress professionally but still like yourself. It’s better to be overdressed rather...challenges, your interviewers might.  You’ll probably still be required to give a job talk for a remote interview...