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Showing: 181 - 200 of 532 results

  1. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...one is not available, don’t lose hope! It could still work. Take a close look at the antigen or antigenic... through the above steps, you may find that you still have a number of options. If this is the case, you...

  2. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...improvements Miniature CRISPR-Cas systems By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus...

  3. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...The titer is tested following each immunization until the desired level of antibodies is reached - typically...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...

  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...environment” with a strong focus on publishing, but still made it so she could “get exposure to the drug discovery...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

  7. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...administration information. Carefully read over all parts until all aspects are understood. While reading the funding...research project grant application" by the NIH Still want more information on the application process...

  8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...proteins. Discovery of anti-CRISPR proteins That is, until the discovery of anti-CRISPR proteins in 2012 (Bondy-Denomy... RNPs, AcrIIA4 reduced off-target editing while still allowing on-target editing (Shin et al., 2017). ...

  9. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...make sure yours counts. Dress professionally but still like yourself. It’s better to be overdressed rather...challenges, your interviewers might.  You’ll probably still be required to give a job talk for a remote interview...

  10. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...include embryonic lethality, a short lifespan, or infertility and can arise due to copy number, expression ...would get both mutations onto the same chromosome. Until crossing over occurs between the two loci, F2 and...

  11. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... that is useful, but not 100% complete, we will still make this data available as a partial sequence with...Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, ...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...gRNA complex binds the target DNA without cleavage until it's activated by light. Once activated, Cas9 cleavage...

  13. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...demonstrating low sensitivity to ambient light, and versatile as it can be activated by both low and high-intensity...

  14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...move in the right direction, the increase notably still does not meet the suggested postdoc salary ($50,...advocating for change: from developing a strategy to distilling a message for the appropriate audience and communicating...

  15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Figure adapted from Kleinstiver et al., 2016. Until recently, two major elements of Cpf1 characterization...off-target editing in a cell culture model. What we still don't know about Cpf1 In their original paper characterizing...

  16. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...large tags into a precise location in the genome is still inefficient, particularly in human cell lines. Furthermore...large regions of homology enable us to effectively utilize the host cell’s inherent homology directed repair...

  17. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...in neuroscience, this architecture is frequently still an open question. The connections between neurons...new neurons came from nearby neurons. It wasn’t until later time points that longer-range connections ...

  19. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...to both people and the tools we use. Scientists utilize an array of tools that rely on DNA amplification...reference is given, de novo assembly in Geneious can still provide additional insights to resolve the assembly...

  20. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...always be added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use...
Showing: 181 - 200 of 532 results