We narrowed to 214 results for: TIR
-
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....thought they would be working in a lab for their entire career. Yet even success in academia means you’...
-
Neuronal labeling with Spaghetti Monster
TypeBlog PostPublishedAug. 14, 2018, 12:42 p.m....cross-react with other antibodies, or are unavailable entirely. The other advantage is that these tags are small... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m.... the ideal codon for every amino acid across an entire polypeptide. Many researchers have created codon-optimization... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m....to limit the spread of infection throughout the entire population. This property of Cas13a opens up the... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....providing a new gRNA rather than engineering an entirely new protein. This makes it much cheaper to use... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m.... beginning of the coding sequence to delete the entire gene? Perhaps you are more interested in the c-... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....Woodgett. Public Library of Science (PLOS) created a stir earlier this year when it announced its data access... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....basics about CRISPR in our CRISPR guide Browse our entire COVID-19 plasmid collection ... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....to avoid animal use by using semi-synthetic or entirely synthetic VL and VH sequences. Phage display also... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....Fluorescence is observed when these RNAs complex and the entire aptamer can hybridize and fold. (Alam et al., 2017... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m.... et al. (2016). To date, small probes that are entirely genetically encoded do not exist, but the development... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....does follow the general structure and flow of the entire session. I myself have a terrible habit of saying... -
Site Directed Mutagenesis by PCR
TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m.... mutation) in a PCR protocol that amplifies the entire plasmid template. The parent template is removed... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....sequencing technologies could one day sequence an entire genome for under $1,000 (10). The future of sequencing... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....sequencing The only way to quantify off-target effects entirely is to perform whole genome sequencing. While this... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m.... photoswitches OptoXRs are great for activating entire signaling pathways, but what if you’re interested... -
Plasmids 101: Dimers and Multimers
TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m.... on as a dimer, depicted on the right, with the entire monomeric sequence duplicated in tandem, resulting... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....solutions that could save both your lab and the entire research enterprise time and funds. It’s time to... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....real-time correspondence via e-mail throughout the entire year. In addition to their direct training role...