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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18 results for: TIR

Showing: 1 - 18 of 18 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...needed. Add stir bar and stir slowly at 4 °C for 1 h, then keep at 4 °C for 3 h without stirring to allow...controller Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents...PEI powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...deionized water and adjust to a final volume of 1000 mL. Stir at RT until fully dissolved. Pro-Tip This step is... challenging due to the high viscosity of PEG. Stirring under medium heat will promote faster dissolution... to ~7.4. Autoclave or sterile filter. Pro-Tip Stirring during the cooling period is recommended or the...proceed overnight at 4 °C if needed. Transfer the entire sample to 3 x 500 mL conical bottles and centrifuge...

  2. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant...presence of particles still in solution. Continue to stir until all particles have dissolved. This may take...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...wash and add RNase-free water or TE as soon as the entire tube is dried but while the white pellet is still...Pro-Tip To avoid multiple freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of...

  4. Lentivirus Production

    Type
    Protocol
    ...controller Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high glucose, Corning... of powder in 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...

  5. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...portion of the insert, but we do not sequence the entire plasmid. Addgene strongly recommends that you...Make sure the primer only anneals once within the entire construct. Is the primer an appropriate distance...

  6. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying from a plasmid or...plenty of starting material. We recommend using your entire PCR reaction and 1μg of recipient plasmid. It is...

  9. General Transfection

    Type
    Protocol
    ...of powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...

  11. Pipetting Protocol

    Type
    Protocol
    ... you can slowly release the plunger. Remove the entire pipette from the container making sure not to touch...

  17. AAV ddPCR Titration

    Type
    Protocol
    ...liquid up and down 10–20 times. Gently cover the entire dilution plate with Microseal adhesive seal - do...

  18. CRISPR Library Amplification

    Type
    Protocol
    ...can dramatically plateau and sometimes fall off entirely if the column or reagents are significantly overloaded...
Showing: 1 - 18 of 18 results