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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 214 results for: TIR

Showing: 1 - 20 of 214 results

  1. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... (Zhang et al., 2015). In this system a modified TIR-1 protein can degrade degron-tagged proteins in an...Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen lab. SapTrap...Project: The Complete Gene Expression Map of an Entire Nervous System." Neuron 99.3 (2018): 430-433. PubMed...

  2. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...Internal Reflection Microscopy (TIRF) Total Internal Reflection Microscopy (TIRF) is a technique used to only...Internal Reflection Fluorescence (TIRF) microscope can be used. TIRF only excites fluorophores within ...the microscope objective evenly illuminating the entire (wide) field of view. Fluorescent light traveling...microscopy project excitation light through the entire axial volume of a sample. Therefore, when a large...limiting photobleaching. Similar to widefield, the entire field of view is excited at one time and captured...light is projected from the objective so that the entire field of view is bathed with an even illumination...emission light from fluorophores higher in the sample. TIRF is also very gentle on the sample as the majority...

  3. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...expressed. Each cell remains the same color for its entire lifetime and daughter cells retain the same color...expressed in a single cell maintains its color over the entire length of the cell, even axons of up to 100 microns...downstream of a neural-specific promoter, or the entire fish could be labeled by injecting Brainbow at ... lines that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...they used CRISPR to create OsTIR1-expressing cell lines by inserting OsTIR1 into the "safe harbor" AAVS1...pooled libraries enable researchers to screen the entire genome for genes that regulate a wide variety of...the NCC-1 library of approved drugs against the entire kit; a parallel analysis that successfully identified...inhibitors are often non-selective, affecting the entire genome and not specific loci. To overcome this ...expressed in a cell that also expressed auxin-sensitive TIR1, any protein fused with their AID tag could be targeted...AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor...

  6. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    .... In this event, the degron domain shuttles the entire tagged protein to the proteasome for degradation...must be AID-tagged with the 25 kDa tag. Next, the TIR1 F-box protein must be expressed (inducibly, ectopically...ectopically, stably… it’s your call). The TIR1 F-box protein will associate with the endogenously expressed...of auxin hormone indole-3-acetic acid (IAA), the TIR1 F-box associated E3 ligase complex will ubiquitinate...

  7. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...focus on our development instead of building the entire ecosystem Proven performance with other bioinformatics..., so our QC team could initiate and monitor the entire pipeline. Benefits of Nextflow for bioinformatics...pieces of software that depend on each other for the entire package to run. The small, portable system provided...process was added, we progressively constructed the entire workflow in the Nextflow script. With our completed...

  8. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...the success of the company is attributed to the entire Addgene family; the depositors and requesting scientists...headed to the Museum of Science in Boston, where the entire Blue Wing was set aside for the event. As guests...family Perhaps the most heartfelt portion of the entire night was when Melina and Ken called their parents...

  9. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...Testing AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: EGFP-expressing...

  10. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...appropriate plasmids, and code the entire page. That would be entirely too much work for one person, it ... expert) the end result is high quality and the entire process is completed more efficiently. Listen to...

  11. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...primarily negative. Stupid, overwhelmed, discouraged, tired…words that indicated there were many barriers between...paper.  Of course, when you think about writing an entire scientific paper, you may feel it’s overwhelming... writing. You can use them to help you write an entire manuscript, or you could use them to write alt ...

  12. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...Passion? A love of learning? No. Or at least not entirely. What truly unites us all is the frustration of... to the day before, which got rid of the issue entirely. In many cases, the answer isn't to work harder...ability to focus, especially on days when I was tired, stressed, or rushing. I would spend the time quietly...

  13. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...selective plane illumination, TIRF or spinning disk confocal use entirely different excitation power regimes...Plasmids for Subcellular Localization Browse the entire Fluorescent Protein Collection ...

  14. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...system relies on a transport inhibitor response 1 (TIR1) expressed under a desired promoter with a specific...) sequence attached to your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 proteins...ubiquitin ligase complex. In the presence of auxin, this TIR1 complex binds to the degron sequence ultimately ...

  15. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Most organisms have less than five enzymes in the entire proteome that are biotinylated. In E.coli for example...responsible for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein...recall, BirA biotinylates only one lysine in the entire E.coli proteome. Using this knowledge, scientists...

  16. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...which is ideal for observing and manipulating the entire developmental cycle. A majority of studies involving...most significant advances is the sequencing of the entire axolotl genome, revealing an abundance of genes...year 2000, becoming the first plant to have its entire genome sequenced (Koornneff and Meinke, 2010). ...

  17. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...idea, but they might feel disconnected from the entire scientific process.  I've spent a lot of time debating...debating how to fix this.  As scientists, we work tirelessly in the lab where the groundbreaking research ...training as an academic scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching...
Showing: 1 - 20 of 214 results