...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...

...backbones containing GFP variants used by Sattarzadeh et al., as well as a wide variety of other fluorescent...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ...

...opportunities or to find oneself unsure of the next steps. Harvard PI Angela DePace has put personal development at...conversation. As the conversation proceeds through the various sections, the trainee speaks first for each section...assessment or critique. When trainees ask for help with various issues, the PI is responsible for determining meaningful...

...London), Dr. Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking...Church and his family spend family reunions in various wildernesses and enjoys “three generations walk...

... these various model systems should allow Nabavi and coworkers to test inhibitors of a variety of signaling...the lungs is relatively well known, there are a variety of lesser known mesothelioma subsets. Peritoneal...

...scale: Look at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer...). It’s just such a great collection with such variety.” In most cases, backbones are available to attach...

... is composed of identical repeat sequences and variable spacer sequences. The array is transcribed and...in the non-target strand of DNA to be cut, which varies between CRISPR systems. Cas proteins won’t cut ... CRISPR by mutating these sequences. Pro tip! Various labs have created Cas9 enzymes with looser PAM ...

...developing transgenic mice that express camelid variable domains (aka nanomice), which gives researchers...modifications made to generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

...efficiency in PAM-in and PAM-out configurations with varied nick distances (40 - 130 nt). From these results...H840A are potent editors, but their indel profiles vary. In the PAM-out configuration, D10A tends to generate...Using a D10A nickase to insert an EcoRI site in various spots between the nick sites, they observed >20%...

...to the beat of their own drum. They have a wide variety of possible genome types that can be either double...positive sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...proteins. RNA viruses replicate their genomes using a variety of RNA or DNA intermediaries, depending on the ...

... confirm expression in target cells. While the various AAV serotypes can be used to achieve different ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

...Streptomyces, a prokaryotic genus capable of producing a variety of natural products. Recently, this group optimized...base-editing efficiency using XMAS-TREE-based methods at various genomic loci in four different categories. ...  Various Addgenies...

...Polyclonal antibodies produced in animals may be variable among animals and bleed dates. The development...as other affinity reagents such as single-chain variable fragment (scFvs) and nanobodies. Addgene will ...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080...

...pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super ecliptic pHluorin...fixed cells. Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein...

...Professor at the Lab for Innovation Science at Harvard, wanted to see how new tools drive innovation in... their gene sequence may have slight or large variations depending on how closely related these species...percentage were not good predictors. There is not much variation in GC content between many organisms and DNA synthesis...

...cilia. They’ll put a variety of plasmids to work to explore the effect of various OCRL1 mutations on cell... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire...

...researchers have been able to study the effects of various stimuli on global translation, but a visual technique...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...represent powerful tools for studying translation in various cell types and physiological states. References...

...used N- and C-terminal dronpa fusions. With these various designs, Xin has been able to develop photoswitchable...has demonstrated the utility of these FLiPs in a variety of research applications and particularly notes...be able to use similar designs to create a wide variety of enzymes and proteins with inducible activity...

...blogger Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent...Joachim Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but...Lambert! Talley Lambert is a Research Associate at Harvard Medical School. He manages the Cell Biology Microscopy...

...never completely eliminate the risk that a minor variation in experimental conditions will derail a research...and why other publishers and organizations have various different versions of checklists and guidelines...Elsevier after her postdoctoral studies, and has held various editorial roles on review and primary research ...