We narrowed to 644 results for: Vars;

...constructs improved editing in various cell types, although efficacy varied among target sequences. The ...they expanded CGBE target scope by creating BE variants with other Cas9s that recognize different PAMs...capable of predicting editing outcomes of their various CGBEs at different target sequences and in different...

...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

...have a variety of downstream effects including possibly decreasing the number of postdocs various academic...Program in Biological and Biomedical Sciences at Harvard University. Ben and Niroshi have tons of great ...

... Biological and Biomedical Sciences program at Harvard University to get the scoop on the ups and down...candidate in the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...of the PhD program in Genetics and Genomics at Harvard Medical School, and is currently a fellow in clinical...

...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...describes how to target and use FRET flurophores for a variety of applications. While editing this post, I learned...

... Biological and Biomedical Sciences program at Harvard University and pick their brains on how they've...candidate in the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...of the PhD program in Genetics and Genomics at Harvard Medical School, and is currently a fellow in clinical...

...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...

...backbones containing GFP variants used by Sattarzadeh et al., as well as a wide variety of other fluorescent...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ...

...opportunities or to find oneself unsure of the next steps. Harvard PI Angela DePace has put personal development at...conversation. As the conversation proceeds through the various sections, the trainee speaks first for each section...assessment or critique. When trainees ask for help with various issues, the PI is responsible for determining meaningful...

...London), Dr. Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking...Church and his family spend family reunions in various wildernesses and enjoys “three generations walk...

... these various model systems should allow Nabavi and coworkers to test inhibitors of a variety of signaling...the lungs is relatively well known, there are a variety of lesser known mesothelioma subsets. Peritoneal...

...scale: Look at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer...). It’s just such a great collection with such variety.” In most cases, backbones are available to attach...

... is composed of identical repeat sequences and variable spacer sequences. The array is transcribed and...in the non-target strand of DNA to be cut, which varies between CRISPR systems. Cas proteins won’t cut ... CRISPR by mutating these sequences. Pro tip! Various labs have created Cas9 enzymes with looser PAM ...

...developing transgenic mice that express camelid variable domains (aka nanomice), which gives researchers...modifications made to generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

...efficiency in PAM-in and PAM-out configurations with varied nick distances (40 - 130 nt). From these results...H840A are potent editors, but their indel profiles vary. In the PAM-out configuration, D10A tends to generate...Using a D10A nickase to insert an EcoRI site in various spots between the nick sites, they observed >20%...

...Streptomyces, a prokaryotic genus capable of producing a variety of natural products. Recently, this group optimized...base-editing efficiency using XMAS-TREE-based methods at various genomic loci in four different categories. ...  Various Addgenies...

...Polyclonal antibodies produced in animals may be variable among animals and bleed dates. The development...as other affinity reagents such as single-chain variable fragment (scFvs) and nanobodies. Addgene will ...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080...

... confirm expression in target cells. While the various AAV serotypes can be used to achieve different ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

...pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super ecliptic pHluorin...fixed cells. Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein...

...to the beat of their own drum. They have a wide variety of possible genome types that can be either double...positive sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...proteins. RNA viruses replicate their genomes using a variety of RNA or DNA intermediaries, depending on the ...