We narrowed to 462 results for: bli
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TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....at Addgene have gone virtual! 2. Practice public speaking For almost any science career, there will... will be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving...
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Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....promise to be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and...library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor... -
Trading Spaces - Visiting Scientist Edition
TypeBlog PostPublishedAug. 22, 2023, 1 p.m....researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...expect to generate data that will contribute to a publication? A grant? A patent? It is never too early to ... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....this screen successfully differentiated cells resembling all three germ layers (ectoderm, endoderm, and...amplification, refer to the Zhang lab’s previously published protocol and its relevant modifications on the... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m....vectors VSV-G and psPAX2, which were used in a publication by Xiuyuan Ou, et al. that characterized the ... requested these plasmids from Addgene. This publication then resulted in the development of a new plasmid... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....do just this. In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....libraries are popular tools for genetic screens, enabling genome-wide interrogation of the genes required...are refractory to lentiviral transduction, now enabling forward genetic screens in cells with few genetic... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate in the biotech revolution...detect compounds in the environment, and openly publish protocols so that anyone can engineer yeast to ... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....Davidson and Roger Tsien played enormous roles in enabling researchers to utilize and develop these tools...Update (7/17/2018): Check out Fatima's recent publication on her HMO work in Cell Chemical Biology! Additional... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....PMCID: PMC6070313. Find the plasmids from this publication at Addgene! Additional Resources on the Addgene...pages Find plasmids from the BioBits BrightTM publication here ... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....nucleofection The in vivo editing experiments in this publication were performed using a technique called nucleofection...Additonal Corn Lab Members who worked on this publication: References 1. Richardson, Christopher... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....available. With new fluorescent proteins being published every month, deciding on the best protein for ...common use (particularly not the most recently published ones), they can be a good starting point. In many... -
Advice for Scientists Starting a Lab
TypeBlog PostPublishedApril 29, 2014, 1:43 p.m....In the blink of an eye, the long days in the lab as a graduate student and postdoc come to an end and...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....to make the sequences of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative...Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded their recombinant... -
Fluorescent Proteins 101: Luciferases
TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....imaging Improving Well-established High-throughput assays Available Well-established Natural and engineered... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....resulting in a fluorescent signal. Since their first publication in 2012, the Nagai laboratory has assembled a...sensitivity and faster imaging compared to previous publications. They also developed calcium, cAMP, and ATP ... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene! Addgene was established as an e-commerce company, which helped it stay...