We narrowed to 214 results for: gnal
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TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....Molecular Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy...
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New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....Marvin E., et al. "A protein-tagging system for signal amplification in gene expression and fluorescence... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....negatives: Interactions leading to detectable reporter signals depend on a number of factors, including protein... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....of a bacterially derived in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine motifs... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....inducible translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins in 2004... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....HIV components: LTRs, PPT, RRE, and psi packaging signal. Viral components typically total <1.5kb, which... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....stress response circuit by integrating neuronal signalling and triggering release of the stress hormone ... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m.... activation of the fluorophore. The fluorescent signal can be used as an indicator to determine whether... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m..... It shows, base-by-base, the average amount of signal that disagrees with the reference sequence derived... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....3-5 nucleotide PAM sequence serves as a binding signal for Cas9 and this sequence is a strict requirement... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m....with a phorbol ester activator gave fluorescent signal in the ticker tape from cFos::GFP-iPAK4 within ... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....presence of liver metastasis by producing a detectable signal in urine. The bottom line with plasmid addiction... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....target is inserted, disrupting the EGFP fluorescent signal, for details on this plasmid, see Mashiko et al... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....color as the fused XFP can be used to amplify the signal. Imaging endogenous fluorescence in live animals... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....Gardiner, 2011). As many biological processes and signaling pathways that regulate regeneration and wound ... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....molecules can bind to a single primary, resulting in signal amplification. Another reason is cost effectiveness... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....inexpensive HDMI transmitter that could transmit a signal between a wall. There was a SLIGHT delay, but I... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....particularly interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....result can be quantified by the strength of the signal in comparison to a control sample. Southern blotting...