Skip to main content

We narrowed to 330 results for: iCre

Showing: 181 - 200 of 330 results
  1. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...plasmid components are brought together and microinjected into either the pronucleus or the cytoplasm ...reagents. Other than that, be nice to your microinjectionist, who has the strategically important job of...
  2. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...field require experience. Working in industry: Microbiology On returning from Ghana at the beginning of ...Industry do tend to pay better. I worked in a Microbiology testing facility, where I tested materials for...
  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...ask incredulously, “You seriously didn’t add 3 microliters of 5 mM star anise?” This is something I was ... could see GFP expression with a fluorescence microscope, but the GFP expression was so low (even in the...
  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Ambros and Gary Ruvkun for their seminal work on micro RNAs in C. elegans. The C. elegans work on small...Versatile Genome Editing Using Linear DNAs with Microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics...
  5. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...relatively small amount of DNA (in the nanograms to micrograms scale depending on the experiment), but issues...require quite large amounts of DNA (easily in the micrograms scale and up). But perhaps more importantly, ...
  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...expression. Check out Sano et al to learn how microRNAs can be used to regulate Sendai virus transgene...Using a Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed PMID: 27764162...
  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Light Inducible Dimers (iLID), iLID nano and iLID micro, which differ from each other by their light/dark... 4.7µM for iLID nano and 800nM to 47µM for iLID micro; Figure 1). With this great affinity range, these...BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin Early Endosome ...
  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...available through Addgene. Stovicek, et al. J Ind Microbiol Biotechnol. 2015. PubMed PMID: 26376869 Jensen...one of them called “claudin-like apicomplexan microneme protein” (CLAMP) was shown to be important for...
  9. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...Cytology and Van Leeuwenhoek Centre for Advanced microscopy (University of Amsterdam). He develops, characterizes...
  10. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...and you can find what you need by field (ex: microbiology, neuroscience), or explore other areas such ...
  11. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...excellent posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR...
  12. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ..., leaving all those healthy bugs in the human microbiome alone. (Seriously, is there anything CRISPRs ...
  13. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...via conjugation.   Read the paper in Nature Microbiology Find the Mobile-CRISPRi plasmids Identification...
  14. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ... of interest. For those of you who are not microbiologists, phages are viruses that infect bacteria and...
Showing: 181 - 200 of 330 results