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We narrowed to 43 results for: iCre

Showing: 1 - 20 of 43 results
  1. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Layer 2-3_IT Isocortex 230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT ...Layer 2-3_IT Isocortex 220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex...Layer 2-3_IT Isocortex 230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT ...Layer 4_IT Isocortex 224056 pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA AiP13152 AiE0050m_3xC2 Cre Layer 4_...Layer 4_IT Isocortex 220734 pAAV-AiE0671m_3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_...Layer 5_IT Isocortex 220735 pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_...Layer 5_ET Isocortex 229920 pAAV-AiE0459m_3xC2-minBG-iCre(R297T)-BGHpA AiP14187 AiE0459m_3xC2 Cre Layer 5_...
  2. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express... pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato... when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255 Cre is coexpressed with...Express iCre under from the CB promoter pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and ...pAdx-CMV-iCre-P2A-tdTomato 73351 Expresses iCre and tdTomato from the CMV promoter pAdxEF1-FLPe-tdTomato 73352 Expresses FLPe...
  3. Caltech Systemic Capsids

    Type
    Collection
    ...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA minBG iCre expression iCre Ting 199775 AiP13778 - pAAV-AiE0450h-minBG-iCre...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) minBG iCre(R297T) expression iCre AIBS , Ting 199776...pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) minBG iCre(R297T) expression iCre AIBS , Ting 199777...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) minBG iCre(R297T) expression iCre AIBS , Ting 214862...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) minBG iCre(R297T) expression iCre AIBS , Ting PHP.S... Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV ...
  4. Microbiology Resources

    Type
    Collection
    ...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene’s ...not listed here. Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...repository contains plasmid tools for a range of microbial fields, including bacteria, viruses, parasites...species you work with, or check out our additional microbiology resources. The plasmids Addgene distributes ...cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause. The standard...
  5. Brain Armamentarium

    Type
    Collection
    ...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...Capsid) 230681-AAV5 AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA AiE2116m is an enhancer sequence, designed...Gradinaru 199777-PHPeB AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre...199776-PHPeB AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre...Gradinaru 199775-PHPeB AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre...Gradinaru 164450-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in...
  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...your experimental results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination...codon usage will improve expression. For example, iCre or FLPo in mammalian cells may facilitate higher...
  7. Recombinases AAV Preps

    Type
    Collection
    ...Carmichael 214848 AiP13780: pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780) minBG N/A 2 Allen Institute...pAAV-EF1a-C-CreintG EF1a none 1 Connie Cepko 69916 pAAV.cTNT.iCre cTnT tdTomato 9 William Pu 203842 pAAV-Ef1a-fDIO-eGFP...Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none 1, PHPeB Hongkui Zeng 140136 pAAV-EF1a-iDreV...
  8. Brain Initiative Collection

    Type
    Collection
    ...Lin Tian 164450-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in...pAAV-EF1a-iCreV can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase...
  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EMTB-mNeonGreen Microtubules EMTB mNeonGreen Dorus Gadella 137803 EMTB-mTurquoise2 Microtubules EMTB mTurquoise2...mTurquoise2-MapTau Microtubules MapTau mTurquoise2 Dorus Gadella 137808 mNeonGreen-MapTau Microtubules MapTau mNeonGreen...mCh-alpha-Tubulin Microtubules alpha-Tubulin mCherry Gia Voeltz 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi...26741 EMTB-3XGFP Microtubules ensconsin GFP William Bement 26742 EMTB-mCherry Microtubules Ensconsin mCherry...18aa-Tubulin Microtubules alpha-Tubulin mClover3 Michael Lin 74256 pKanCMV-mRuby3-18aa-Tubulin Microtubules alpha-Tubulin...mScarlet3_N1 Microtubules EB3 mScarlet3 Dorus Gadella 189768 pmScarlet3-alphaTubulin_C1 Microtubules alpha-Tubulin...pTubulin-miRFP670nano3 Microtubules alpha-Tubulin miRFP670nano3 Vladislav Verkhusha 61196 pCMU-MTUBr Microtubules (Plants...
  10. Optogenetics Plasmid Collection

    Type
    Collection
    ...Find optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and...Optogenetics Plasmids Optogenetics Plasmid Collection Microbial Opsin Plasmids Optical Switch Plasmids Additional...controlled by light. Many of these plasmids encode for microbial opsins, which are membrane channels and pores ...neurons in a precise manner. One of the first microbial opsins to be adopted as an optogenetic tool is...expression and membrane targeting in host cells. Other microbial opsins, such as the Halorhodopsins, Archaerhodopsins...research? Find related posts on Addgene's blog. Microbial Opsin Plasmids Browse, sort, or search the table...
  11. Fluorescent Proteins: FRET

    Type
    Collection
    ...FLIM (FLIM-FRET)? Leica Microsystems Blog . https://www.leica-microsystems.com/science-lab/life-science...Link opens in a new window) Nikon MicroscopyU: Basics of FRET Microscopy (Link opens in a new window) Fluorescent...efficiency can be measured using fluorescence microscopy or spectroscopy by exciting the donor and detecting... measured using fluorescence lifetime imaging microscopy (FLIM) or FLIM-FRET, where the increased energy...
  12. E11 Bio PRISM Collection

    Type
    Collection
    ... for use in expansion microscopy, allowing for super-resolution light microscopy. In brain tissue, these... sparse set of neurons across 10 million cubic microns of mouse hippocampus using PRISM. Adapted from ...). These reagents have been used in expansion microscopy workflows to achieve high-resolution optical ... Multiplex imaging of barcodes with expansion microscopy and reconstruction of neurons with machine learning...
  13. Fluorescent Protein Guide

    Type
    Collection
    ...Talley Lambert. Nikon MicroscopyU (Link opens in a new window) Olympus Microscopy Resource Center (Link...Link opens in a new window) Leica Microsystems Resources for Microscopy in Life Sciences (Link opens in ...
  14. Antibody Guide

    Type
    Collection
    ...conjugate or conjugation process. Sodium azide, an antimicrobial agent, can block some amines needed for conjugation...In those cases, thimerosal can be used as an antimicrobial agent. Other chemical interferences with conjugating...strong interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in..., and wash. Preserve sample and image using a microscope. Special considerations Many of the steps in ...in this protocol vary depending on assay and microscopy techniques used, so be sure to read materials specific... flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647...
  15. Viral Production

    Type
    Collection
    ... (Addgene viral prep # 84445-AAVrg). Electron Microscopy The ratio of empty to full (i.e., genome containing...vector preparations was quantified with electron microscopy after negative staining. Empty vector particles...than full vector particles. Figure 3: Electron micrograph of AAV vector preparation shows that the vast...cultivation, cells are routinely checked for signs of microbial contamination. Purity When possible, all plasmids...
  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...imaging activity in neuronal populations and microcompartments. Nat Methods. 2019 Jul;16(7):649-657. Douglas...quantitative measurement of the local calcium microdomain in the endoplasmic reticulum. Biophys Rep (2019... Zinc FRET sensors ZapCV2 and ZapCV5 Droplet Microfluidic Flow Cytometer For Sorting On Transient Cellular...using a fast indicator evolved for two-photon microscopy. Cell. 2022 Aug 16. pii: S0092-8674(22)00916-...fluorescent voltage sensor to detect neural activity (FlicR1) A Bright and Fast Red Fluorescent Protein Voltage...electrophysiology in mammalian neurons using engineered microbial rhodopsins. Nat Methods. 2014 Aug;11(8):825-33...
  17. Immunology Research Plasmids and Resources

    Type
    Collection
    ... sense conserved microbial molecules during an infection. When TLRs sense a microbial pathogen, they trigger...physical barriers such as the skin and mucosa, antimicrobial peptides and proteins in the blood such as interferons...glycoprotein 1, zinc-binding ZA2G, ZAG B2M beta-2-microglobulin - CALR calreticulin CRT, FLJ26680, RO, SSA, ...receptor 1 C5A, C5AR, C5R1, CD88 CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39, FALL39, HSD26...receptor-like CGRPR, CRLR CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39, FALL39, HSD26...guanylin) GUANYLIN, GUCA2, STARA HAMP hepcidin antimicrobial peptide HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1...
  18. Plasmids for Stem Cell Research

    Type
    Collection
    ...Oct4, Klf4, Sox2, c-Myc, Nanog, Lin28, NR5A2, and microRNA 302/367 in three different combinations of reprogramming...8. Parmar Fibroblasts Neurons Lentiviral Human MicroRNA-mediated conversion of human fibroblasts to neurons... Human Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion of Fibroblasts. Neuron...neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways. EMBO Mol ...
  19. CRISPR Plasmids - Tagging

    Type
    Collection
    ...proteins. The PITCh system depends on MMEJ (microhomology-mediated end-joining), an alternative DSB repair...because MMEJ utilizes very short (around 20 bp) microhomologies as homology arms. The system consists of two...with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and gRNA target sites. The first ...
  20. CRISPR References and Information

    Type
    Collection
    ...integrates information from Cas-OFFinder and Microhomology predictor. Developed by the Jin-Soo Kim Lab ...D10A (pX335) PDF, 255 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive...pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...
Showing: 1 - 20 of 43 results