We narrowed to 330 results for: iCre

...remain in the delivery of these agents, CRISPR antimicrobials could become our newest line of defense against...the growing problem. Above all, we need new antimicrobials, ideally ones for which the development of ...process essential to most bacteria, custom CRISPR antimicrobials can target specific sequences in a single virulent... Bikard et al. developed sequence-specific, antimicrobial, plasmid-based, CRISPR systems (Find the Citorik...particles using a helper system. These CRISPR antimicrobials successfully resensitized a beta-lactam resistant...observed substantial resistance to the CRISPR antimicrobials, and they suggest that targeting multiple resistance...these techniques is efficient delivery, as the antimicrobial must reach essentially every cell or else small...

...looking at’ to ‘listening to’ microbes But seeing microbes through a microscope is not the only way to visualize...nope, not at the American Society for Microbiology 2018 Microbe meeting. Soon after I arrived, I was looking...improv workshop. These paper microscopes work as conventional microscopes and give a similar optical quality...140X and 2 micron resolution). I have worked many hours in a dark room with a confocal microscope, yet I ...through a paper-based, affordable, and portable microscope called the ‘foldscope’ (Dr. Prakash, Stanford...when looking down a paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful...Caltech presented an exciting way of listening to microbes by using non-invasive methods for cellular imaging...

... switch microbiota/biomarker sensors here Studying the bee microbiome using the Bee Microbiome Toolkit...what the microbes are doing. But aside from sequencing, scientists can also study the microbiome by using...reporter microbes. Let’s take a look at a few of these methods below. Engineering gut microbes in situ...monitor the microbiome with sequencing is vast, the ability to genetically alter microbiome members is ...number of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome, James Collins...developed the Bee Microbiome Toolkit (BTK) to study bumble bee and honey bee microbiome. The toolkit consists...the bee microbiome, these broad-host-range plasmid could also be used to study other microbiome systems...

... techniques like super-resolution microscopy or electron microscopy. Since its introduction in 2015 by...publication.         Why Expansion Microscopy?  Traditional light microscopes are limited in the level of resolution...for EM or SRM? Enter Expansion Microscopy. What is Expansion Microscopy?  ExM provides a straightforward...Expansion Microscopy (ExM) promises an easier, more accessible way to image biological features previously...synapse by conventional light or fluorescence microscopy (Dani, et al., 2010). How do you overcome this...this resolution limit? Electron Microscopy (EM) is one option, but the equipment needed for EM is not something...targets you can label in a sample. Super-resolution microscopy (SRM), a family of imaging techniques developed...

...word out about a particular microorganism, microbial technique, or microbial technology, sign up to write...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be sure... resource page for the Microbiology community. On our new curated Microbiology Collection page, you can...resources for microbial research. Kits, guides, plasmids, and more We have Plasmid Kits for microbial work with...out how to use genome editing techniques in your microbe of choice. For more help with CRISPR, read our ...find plasmids for your work, plasmids in the Microbiology Collection are sorted by organism (see screenshot... microbiology...

...Light sheet fluorescence microscopy vs. confocal microscopy In confocal microscopes, optical sectioning of... light microscopy, particularly related to the neurosciences.  These developments in microscopy coupled...One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). The underlying ...light sheet fluorescence microscopy Modern light sheet fluorescence microscopy was first pioneered by Voie...cleared rodent brain, the Ultramicroscope was developed using a stereomicroscope to capture large field-...www.lavisionbiotec.com/ultramicroscope-overview.html http://www.zeiss.com/microscopy/en_de/products/imaging-systems...techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture of whole...

...Fluorescence microscopy techniques   Fluorescent Widefield Microscopy Fluorescent Widefield Microscopy is the...photon microscopes. Total Internal Reflection Microscopy (TIRF) Total Internal Reflection Microscopy (TIRF...fluorescent microscopy. Collimated (non-converging or diverging) excitation light exits the microscope objective...Epi-fluorescence microscopes. (See left image above for an example image). Point Scanning Confocal Microscopy Point...Point Scanning Confocal Microscopy was the first fluorescence microscopy technique to incorporate optical...Parallelized Confocal Microscopy (Spinning Disk) Parallelized Confocal Microscopy (Spinning Disk) increases...confocal microscopes use parallelization to enhance performance. In spinning disk microscopy, a metal ...

..., we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...attended the American Society for Microbiology's annual meeting (ASM Microbe 2016) for the first time. Our...on the horizon for microbiology research and we’d love to help more microbiologists share these valuable...resources! Developing the Addgene microbiology community #ASMMicrobe2016 was a great opportunity for Addgene... out Our Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...recently begun expanding our presence in the microbiology community. For our first concrete steps into...little bit of what we learned. Check Out Our Microbiology Page! The start of the conference After setting...

...Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia. PNAS 97, 1813–1817...machines that can extend and retract over many microns in response to pH changes without the need for ...chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer of many copies of monomeric...bodies could also perform as actuators in microelectromechanical systems (MEMS) devices. They could be functionalized...

...backstage team. Meet the microenvironment, a tumor’s backstage team. Microenvironments are cellular regions...cancer microenvironment Ever since Stephen Paget’s “seed and soil” theory in 1889, microenvironments have...Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009): 9-17...specialized structures. Tumors are surrounded by microenvironments and what encircles a disease is highly prone...precisely that for breast cancer. What are tumor microenvironments made of?  A tumor’s genesis is divided into...initiation, progression, and metastasis. The microenvironment’s physiology is deeply connected to these 3... soup of life encircling the tumor. How do microenvironment enzymes and metabolic reprograming influence...

...Acoustic reporter genes for noninvasive imaging of microbes in mammalian hosts Article contributed by Lukas...are beginning to find connections between the microbiome and diseases, the immune system, and other vital...vital systems in the body. When a microbe travels through a host organism, the expression of some genes...methods for visualizing the location and function of microbes rely heavily on fluorescent protein genes. These...provide a major benefit to scientists studying microbiome systems that are potentially problematic for ...podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators...

...Larsen New serotype AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) AAV PHP.eB Recombinases...viral prep AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) AAV PHP.eB Recombinases... New viral prep AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) AAV PHP.eB Recombinases...

...continents and research diverse topics including microbiology, brain injury, drug discovery, and synthetic...Cayetano Heredia, Peru Patricia Sheen, Professor in Microbiology, and her group are interested in understanding...biofilms will then be monitored using fluorescence microscopy and FACS. In an additional approach, plasmids... proteins and a new two-photon laser scanning microscope will allow Tsakanova to image cortical neurons...Additionally, optogenetic plasmid tools and non-linear microscopy methods will be used to stimulate and manipulate...extreme life on earth including extremophile microorganisms, biomarkers, and recently, cancer research ... employing synthetic biology resources and microfabrication technologies to generate a self renewable ...

... viral prep AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) AAV-PHPeB Recombinase...

...green fluorescence (Figure 2). Donor cells express iCre with a mitochondrial anchor and a TEV cleavage site... al. (2025). Probing condensate microenvironments with a micropeptide killswitch. Nature, 643(8073), 1107...Red calcium indicators PinkyCaMP and FRCaMPi Micropeptide killswitch for biomolecular condensates AAV-...including confocal and widefield imaging, two-photon microscopy, and fiber photometry. Plus, both designs offer...https://doi.org/10.1371/journal.pbio.3003048   Micropeptide killswitch for biomolecular condensates By Emily...condensate, the killswitch disrupts its unique microenvironment, immobilizing the components and altering ...choose from! Find plasmids to probe condensate microenvironments here! Mensah, M. A., Niskanen, H., et al....

...cells? 1. Epifluorescence microscopy This technique uses a fluorescence microscope where the light source...proteins or dyes. 3. Two-photon microscopy Two-photon excitation microscopy is a fluorescence imaging technique...regarding immune cell mobility than the static microscopy images of chemically coloured lymphoid organs...specimen and the excitation light passes through the microscope objective lens on its way toward the specimen...occurs only at a chosen focal volume and thus the microscope captures only fluorescence coming from this volume...below for an example of two-photon intravital microscopy from PLoS Pathogens - Kamenyeva et al, 2015. ...particular stimulus. Using an epifluorescence microscope and GFP fusion proteins controlled by promoters...

...limits the use of microscopy in open science. But the new field of open-source microscopy seeks to change... Open-Source Microscopy Sharing Hardware vs. Sharing Software In the field of microscopy, where image ...Stirling, et. al, "Robotic microscopy for everyone: the OpenFlexure microscope," Biomed. Opt. Express 11...Since its early years, the light microscope has been a powerful tool in almost all disciplines. In biology...experiments. Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (...openUC2 (Diederich et. al., 2020) aim to make microscopy as a tool accessible to practically everyone....started my PhD, I have been convinced that most microscopy techniques can be replicated with commercially...

...competition between microorganisms for available resources. This competition drives microbes to develop survival...mechanisms. Some microbes even make their own antibiotic compounds. In such a case, the microbe itself would...surrounding microbes would be affected (possibly killed), thus increasing the initial microbe’s chances of...to Use Our Microbiology Pages Additional Resouces on Addgene.org Check Out Our Microbiology Pages ... guest blogger, Jennifer Rivers, a research microbiologist specializing in bacteriology and drug resistance...positive force for public health. Visit Addgene's Microbiology Pages What is antibiotic resistance? Antibiotic...Antibiotic resistance is defined as microbial survival despite exposure to antibiotics designed to kill them...

...  Opto-katanin: a light-powered microtubule-cutting sword Microtubules are one of the most important components...recruited to microtubules under blue light, removing tubulin dimers from the microtubule body. B) Schematic... several strategies are available to disrupt microtubules in cells, they have various disadvantages and...and limitations – for example, microtubule-destabilizing drugs are impossible to target to specific subcellular...published a new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022...Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of Opto-katanin constructs...under CC-BY 4.0 license. A naturally occurring microtubule-severing protein, Katanin is of course named ...

...track them. We have a number of microscopes, including an automated microscope but we need to develop the ...skills at the regular microscope, and the scripting skills at the automated microscope  to keep our worms...Working on the Cat Microbiome Project Another project we are working with is the cat microbiome project. This... domesticated cats and learning about feline microbiomes. One of the aspects of this project that makes...project gives them access to rich and complex microbiome data for analysis. And, well, cats... everyone...possible, but one of our members took an Arduino microcontroller and a motor controller and made it spin again...re-use it as a platform for monitoring homemade microfluidic chips using the fantastic optics and fluidics...