We narrowed to 230 results for: pET

1. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   Published

   July 1, 2022, 1:15 p.m.

   ... needed. Traditional media formats are quite competitive while digital can be oversaturated. Transition...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...that inhibit BiFC in your cellular system. BiFC competition analysis can be used in lieu of negative controls...

3. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   Published

   July 1, 2025, 1:15 p.m.

   ...protocol. Are any of your materials expired? Did you pipette carefully? Remember to discard flow-through solutions...

4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...been full of dark matter: enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We...

5. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

   Type

   Blog Post

   Published

   March 17, 2016, 2:30 p.m.

   .... "Minigenomes, transcription and replication competent virus-like particles and beyond: reverse genetics...

6. Course-Based Undergraduate Research Experiences (CUREs)

   Type

   Blog Post

   Published

   Feb. 9, 2023, 2:15 p.m.

   ...Einstein Discovers America. New York./  Callahan, K., Peterson, C., et al. 2022. External collaboration results...

7. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

8. DNA Purification Without a Kit

   Type

   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ...mammalian cells in 30 seconds or less without using a pipette.  Here’s how this method works: nucleic acids are...

9. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...

10. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...for the modular assembly of highly similar or repetitive sequences. Intermediate chaining products (called...

11. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...survive as a researcher because of the tough competition for academic positions and poor grant situation...

12. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...robust research proposal will distinguish between competing models. Comparing model A vs. B is more constructive...

13. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...Finally, if you still find the idea of not holding a pipette again upsetting, there are ways to get back to ...

14. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ...C) installation by base editors. Pathways that compete with the desired outcome are not pictured. ...

15. Transferable Skills Guide: Creativity

    Type

    Blog Post

    Published

    Jan. 18, 2022, 2:15 p.m.

    ...chain shortages. When flasks, conical tubes or pipette tips are backordered for months, we have to have...

16. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...serum immunoglobulins indiscriminately, causing competition for binding sites. In cases like these, antibodies...

17. Viral Vectors 101: Pseudotyping

    Type

    Blog Post

    Published

    April 20, 2021, 2:30 p.m.

    ... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...

18. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... assortment toxins to claim territory or kill competing microorganisms, but did you know bacteria also...

19. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ... abundance relative to cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to...

20. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...But, as so often happens, one gets better with repetition. I learned to look at the event from the company...