Skip to main content

We narrowed to 232 results for: pET

Showing: 181 - 200 of 232 results
  1. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...for the modular assembly of highly similar or repetitive sequences. Intermediate chaining products (called...
  2. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...mammalian cells in 30 seconds or less without using a pipette.  Here’s how this method works: nucleic acids are...
  3. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...
  4. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...
  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... abundance relative to cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to...
  6. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... are performed, the next step is to transform competent E. coli cells and select the positive clones. ...
  7. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate...
  8. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...chain shortages. When flasks, conical tubes or pipette tips are backordered for months, we have to have...
  9. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...serum immunoglobulins indiscriminately, causing competition for binding sites. In cases like these, antibodies...
Showing: 181 - 200 of 232 results