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We narrowed to 352 results for: plasmids 101

Showing: 181 - 200 of 352 results

  1. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids 101: Origin...stability of modified Ti plasmids during plant infection, as well as plasmid yield during preparation ...Agrobacterium carries a “tumor-inducing” or Ti plasmid, which allows it to transfer genetic material into...in two species: Agrobacterium and E. coli. Like plasmids used in other systems, binary vectors are cloned...It’s a lot of information to fit into a single plasmid, so most first-generation binary vectors are quite...pSiM24 are suitable for many applications, the plasmid can also be customized due to its modular structure...Addgene Check out Addgene’s plant vectors! Find the plasmids from this paper at Addgene. Other relevant blog...

  2. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Epitope Availability Antibodies 101: Introduction...Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant antibodies at Addgene   ...

  3. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ... on All Things Plasmid with Our Plasmids 101 Series Get Tips on Verifying Your Plasmid Resources on Addgene.org...Check out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Bonus...recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient plasmid by viewing... of DNA so that it can be easily cloned into a plasmid of interest. You can use similar processes to add... Interest (YGOI) for ligation into a recipient plasmid. Designing primers for PCR based cloning   ...this example). Assuming you are amplifying from plasmid DNA (rather than from genomic DNA or a cDNA library...

  5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    Published
    Aug. 27, 2020, 1:15 p.m.
    ...because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How to Design ...proteins, check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also ...the Addgene blog Browse our Fluorescent Protein 101 blog posts Read our blog post covering an introduction...Resources on Addgene.org Find fluorescent protein plasmids and resources Visit our Molecular Biology Reference...Complementation. Molecular Cell 9:789–798. https://doi.org/10.1016/s1097-2765(02)00496-3 Kerppola TK (2006) Design...Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-...

  6. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...resources on the blog CRISPR 101: Cas9 vs. The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off...its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection ...Molecular Cell, 70(3), 385-394.e3. https://doi.org/10.1016/j.molcel.2018.03.031 Xiao, Y., Luo, M., Dolan, ...and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo, J. ...Molecular Cell, 83(5), 746-758.e5. https://doi.org/10.1016/j.molcel.2023.01.024 Morisaka, H., Yoshimi, K.,...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target...neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn...H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2... has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV...AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471... jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with...post Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...Vectors 101: Viral Applications Adenoviral Vector Production and Troubleshooting Viral Vectors 101: Types...vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early 1 and Early 3) genes .... These genes’ functions are rescued by helper plasmids used during production and/or by genes present...made available by this deletion from the genomic plasmid can be used to house a recombinant sequence (transgene...late genes are usually present in the transfer plasmid for the majority of adenoviral vector systems used...promoters. Mol Ther, 7(4), 526–534. https:/doi.org/10.1016/S1525-0016(03)00023-6. PMID: 12727116. Borkenhagen...Biomedical Sciences. Elsevier. https://doi.org/10.1016/B978-0-12-801238-3.02523-X. Hofmann, C., Löser,...

  10. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...viral vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo intramolecular...contain the full length plasmid.   Figure 1: The full lentiviral plasmid is expected to be >12 kb...in my samples? Plasmid recombination can affect viral vectors (LTRs and ITRs), plasmids with multiple ...other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids and ...browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli Strains blog post. Check... length plasmid. If you find that your bacterial cultures contain a mix of full length plasmid and the...smaller, recombined plasmid around 1.5 kb. When cut with BamHI, the expected linear plasmid is present, but...

  11. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...Chemogenetics Plasmid Serotype Name 119472  AAV5  AV SYN PSAM4 GlyR IRES EGFP   Biosensors Plasmid Serotype...inventory of serotype testing AAV New control AAVs Plasmid Serotype Name 105530  AAV1 pAAV.CMV.PI.EGFP.WPRE.bGH...biosensor AAV inventory New glutamate sensors Plasmid Serotype Name 106176  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A...pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name 104496  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST...of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name 75033  AAV1 pAAV CD68-hM4D(Gi)-...pAAV SYN1 HA-hM3D(Gq) New chemogenetics AAV Plasmid Serotype Name 119741  AAV5  AAV SYN flex PSAM4...

  12. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...

  13. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...Would you buy and use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately...depositing your plasmids encoding antibodies, ScFvs, and nanobodies to Addgene! The plasmids will be made... blog Check out our Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal antibodies...Plückthun, 2015a, b). Recombinant antibodies are plasmid-based and offer numerous advantages, including ...antibodies that you have come to expect from our plasmid and viral services. The first step will be working...working with the community to build a resource of plasmids encoding recombinant antibodies as well as other...through our usual service (see our current antibody plasmid collection) and we plan to introduce affinity reagents...

  14. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate...genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional resources...effects.  Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual...TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches...drugs to further understand their mechanism. These plasmids are available as a collection, facilitating screening...Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments ...

  15. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ... Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your Plasmid Background...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find...appropriately sized product, purify and sequence the plasmid to confirm no mutations were introduced during ...Browse Our Special Collections for Plasmids from Your Field ... plasmid cloning...

  16. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

  17. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...recommended for plasmids up to 25 kb, most cloners should be fine, especially using plasmids in the 10-12...Other Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read...Read our Plasmids 101 Series Additional Resources on Addgene.org Browse Our Molecular Biology Reference...Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...made genomic SDM relatively straightforward, but plasmid-based SDM has lagged behind. While commercial kits... REPLACR (Recombineering of Ends of Linearized Plasmids After PCR). Current site directed mutagenesis...mutation, then use those to PCR amplify the template plasmid. The parental, nonmutated DNA is digested with ...

  18. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...indicators Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype...serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5, AAV8  pAAV-hSyn-mCherry...channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA...55637  AAV1  pAAV-EF1a-Flpo Calcium sensors Plasmid Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-...tips for preparing AAVs Download our viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's...

  19. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While this strategy greatly ...analyzing the plasmid and viral DNA sequences and expression profile at multiple steps from plasmid amplification....2020.618020 Additional resources on the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When ...recombination that are typically employed during viral plasmid amplification (Fischer, KB. 2019). This means that...of this finding, we now include guidance on the plasmid page of DIO/FLEx AAV vectors where this occurs ... mechanism of spontaneous reversion during AAV plasmid amplification. On the left homologous pairs of ... ready to use AAV vectors at Addgene. Find AAV plasmids to use in your experiments. ...

  20. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for expression...protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning.... Now, we've turned some of the most requested plasmids into lentiviral preps to speed up COVID-19 research...Testing AAV inventory for the entire collection! Plasmid Serotype Name Depositor 27056 AAVrg pAAV-Ef1a-DIO...constructs that allow for targeted expression. Plasmid 75470 is now available in AAV1 for Flp-dependent... mCherry reporter. We now also offer AAVrg for plasmid 83898, which targets expression of ChR2 to forebrain...interneurons through the mDlx enhancer element. Plasmid Serotype Name Depositor 75470 AAV1 pAAV-CAG-FLEXFRT-ChR2...
Showing: 181 - 200 of 352 results