We narrowed to 225 results for: poly
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TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...
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Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m.... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....the surface of the protein where the nascent polypeptide emerges from the exit channel. He then said... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....Chemical transfection Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....production: Gag (structural precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m....interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m.... Selecting the Right Antibody Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m.... from the ori. In the elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts. In a plasmid... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m.... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....which contains a promoter, the envelope gene, and a polyA tail. Figure 1: Lentiviral production uses...