We narrowed to 230 results for: poly

...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...

...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...

... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded...

...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

...HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows...

..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

...Chemical transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines...

...resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ...

...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...

...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

...production: Gag (structural precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional...

...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

...which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses...

... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ...

...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...

... interact with mediator complexes and the RNA polymerase at the promoter region of a gene to initiate ...

...they co-expressed Polη, a translesion synthesis polymerase known to preferentially incorporate A bases across...