We narrowed to 227 results for: poly

...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...

...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

...HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows...

... (cap) genes. AAV replication uses the host’s polymerase, but requires Rep proteins to process a double-stranded...

...Chemical transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines...

...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

...resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ...

...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...

...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

...production: Gag (structural precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional...

...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...

...which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses...

...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

...Paolo, et al. "Systemic errors in quantitative polymerase chain reaction titration of self-complementary...

... interact with mediator complexes and the RNA polymerase at the promoter region of a gene to initiate ...

...they co-expressed Polη, a translesion synthesis polymerase known to preferentially incorporate A bases across...

...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke...

... Selecting the Right Antibody Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene...