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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 430 results for: sequence

Showing: 181 - 200 of 430 results

  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...The raw data is then normalized to a reference sequence present in the same amount in all samples (usually...to run multiplex assays, allowing the reference sequence and the gene of interest to be amplified in the...for both the gene of interest and the reference sequence. At the end of the run, each droplet is read as...Complexity of the gene of interest Some gene sequences are harder to amplify and quantify than others...

  2. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately, the vast majority... majority of antibodies are supplied with no sequence information, leading to problems with reproducibility...affinity reagent screening, and next-generation sequencing of hybridomas have opened the door for a new ...

  4. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...sgRNA) that bears complementarity to a 20bp target sequence (Qi et al. 2013). Modifying the CRISPRi system... will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional regulator...Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell, 152...

  5. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...please contact us. Full sequencing of the Viral Genome Next-generation sequencing is performed on viral ...genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient...genomes isolated from the final AAV preparation. Sequencing results are analyzed to confirm the identity ...

  6. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... a genetic sequence and integrate it into any DNA sequence containing the 38-50 bp sequence recognized...they optimized the specific attachment sites via sequence engineering, enabling even more efficient integration...

  7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... effective, ivTRT may be more challenging for sequences with complex secondary structure. Today, multiple.... Miura et al. (2018) suggest using a 50-80 bp sequence around the desired insertion site to search for...C-terminal insertion with Easi-CRISPR. Potential guide sequences (arrows) and their PAM (bold highlighted text)... knock-in design may naturally remove the gRNA sequence or place it far from the PAM; if not, you can ...guide. This design also separates the gRNA target sequences from their PAMs, preventing re-cutting after editing...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ... a ~20 nucleotide targeting sequence and ~75 nucleotide scaffold sequence) is consistent between plants...contain a plant pol III promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos...is commonly tagged with a nuclear localization sequence to enhance targeting to the nucleus, and several...the specifics - promoters used, precise protein sequences or domains, and methods of delivery - the underlying...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...the activator protein cannot bind the promoter sequence anymore and transcription is turned OFF. Negative...protein is present, but cannot bind the promoter sequence and transcription is ON. Once a co-repressor protein... (TetR) can bind to the tetracycline operator sequences (TetO), preventing transcription. In the presence...binds to promoters containing putative operator sequences (CuO) (linked in groups of 6), allowing transcription...which activate their respective UAS and lexAop sequences but are also regulated by QS and quinic acid. ...

  10. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery... genes and siRNA encoded downstream of the UAS sequence are only expressed when Gal4 is expressed. Gal4...multiple genes under the control of UAS enhancer sequences. For example, if you discovered five new genes...

  11. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...protocol before performing single-cell RNA sequencing. The sequencing results covered TFs representing 92% ...average of 206 cells per TF ORF. From this large sequencing endeavor, a map of possible TF-induced cellular...

  12. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences can be used ...plant source), by tuning the length of 5’ leader sequences of a given tRNA to achieve the highest efficiencies...

  14. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    .... Piecing together multiple PCR products using sequence homology is also common. Overlap extension PCR... to splice together two PCR amplicons based on sequence homology, and the subsequent product is then digested...fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis: Letting bacteria...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...longer stretches of sequence could be used. However, a practical limitation is sequencing depth and cost. ...variants with optimal function. High-throughput DNA sequencing is then used to measure the frequency of each...more codons you test in one library, the more sequencing reads you will need. See Fowler et al for more...

  16. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ... strand at each replication fork. Termination sequences present in the chromosomal DNA ensure that the...pSC101 itself. RepA binds to directly repeated sequences called iterons located near the ori and directly...ColIb-P9 plasmid, is complementary to part of the sequence forming this pseudoknot, so its binding prevents...replication may differ in the specialized proteins or sequences necessary for initiation or the mechanisms that...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...platform is compatible with enrichment, single-cell sequencing, and optical pooled screens in human cells. Addgene... improve the specificity of ssAAV, a targeting sequence of miR124T was inserted into the vector to silence...alone or in combination with miR124T targeting sequences, provides a simple yet valuable tool for microglia... REVeRT to functionally reconstituting coding sequences for a range of applications, including CRISPR ...

  18. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...inducible lambda-red gene, and an IPTG-inducible sgRNA sequence targeting the pMB1 replicon on pTargetF (Jiang... this system, you’d first introduce your sgRNA sequence into the pTargetF construct and then introduce... process begins with the addition of the sgRNA sequence in pEcgRNA. Next both plasmids are transformed...pTargetF, we replaced the original 20 bp specific sequences on pTargetF with the ccdB gene containing BsaI...

  20. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...: shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...viral vector for your gene of interest – an shRNA sequence specific to your gene’s mRNA or a sgRNA targeting...
Showing: 181 - 200 of 430 results