...aerosols that are generated (while talking or pipetting) during working in the laminar airflow are also ...providing proper training and guidance to the people working in the lab facility. This can be done by training...talk, laugh, sneeze or cough. Wear gloves while working and discard them after use. Human contact can spread... spread contamination when you are working without gloves or masks. Make sure to change the gloves and...inside the laminar airflow before and while you are working. Perform routine checks for mycoplasma contamination... should be cleaned regularly and dampness of any kind should be avoided. Distilled water provided to maintain...because you are not the first person to face this kind of problem. Educate others when you can and help...

...tool of choice. Nicking sgRNAs PE3 and PE5: These tools use an sgRNA to guide nicking of the unedited...editors bound to sgRNAs only drive nicking (right). Start with nicking sgRNAs ~50 bp from the prime editing... Figure 7: Nicking sgRNAs can be designed to target edited DNA, so that nicking only occurs after... prevents the Cas9 nickase from re-binding and nicking the newly synthesized strand before heteroduplex...BioRender.com. PE3b and PE5b: In this approach, the nicking sgRNA is designed to bind only after the edit is...

... to worm motion. Working on the Cat Microbiome Project Another project we are working with is the cat ...computer scientists, engineers, and biologists working together on a variety of projects including the...cat microbiome project. This is a project looking at the makeup of the bacterial species in the gut of the...expensive to make it practical - but we have members looking at how to re-use it as a platform for monitoring...makerspace somewhere near you. The place to start looking for your local space is the Hackerspace website...Victoria. After a decade at Microsoft Research working on speech recognition, he returned to school to...manufacturing tools – a 3D printer, a laser cutter, woodworking tools, and a metalshop. After two summers in ...

...provide constructive criticism, and ask thought-provoking questions. For example, if you’re a virologist...help build your presentation. For example, when thinking about discussing a figure, jot down the question...poster where the presenter spends the entire time talking to the poster, as opposed to engaging with you?...Even the most prepared of us can find ourselves talking to our posters, so if you find yourself in this...let them know while you appreciate the thought-provoking question, you are unable to answer it at this ...give the “elevator pitch,” of his/her project – kindly let the presenter know you are very interested ...interested in viewing which has drawn a large crowd, making it difficult to see the actual poster or interact...

...while still working towards your degree. For me, experimenting with the career and networking opportunities...workshops and networking events. I took advantage of as many of these resources as I could. Networking and learning... I liked – well, I was used to experiments not working, so just like in lab, I sucked it up and moved ... like a great way to share some of the research taking place at our university with the community. I even...and David Shiffman @WhySharksMatter and see what kind of content they share and produce and how they interact...wanted to do. A few weeks into volunteering, I was talking to a little girl about fossils. After we talked...

...very small homology regions (5-25 bp) for repair, making it easier to construct targeting vectors. Addgene...copies the sequence from a repair template with flanking sequence homology for error-free DSB repair. NHEJ...MMEJ uses regions with 5-25 bp of microhomology flanking a DSB to repair DNA. The DNA ends are chewed back...figure above, short (5-25 bp) regions of homology flanking a double-stranded break yield precise deletion... is likely to introduce additional nucleotides flanking the insertion. If the DNA ends anneal incorrectly...5’ and 3’ microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks...sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus (see figure...

... unbiased potential to find things you are not looking for, but there are also benefits gained by lowering...differentially expressed genes can be the next stage in making sense of such data. These targeted screens can ...ligand (such as when a component of a microbe or a cytokine is added to a cell). With a custom CRISPR screen...example, one could study the cellular response to a cytokine known to cause the up-regulation of an important...cell). In this screen, known components of the cytokine signaling pathway could be targeted. Cells failing...the transferred cells is followed. A benefit of working with surface proteins is also that such proteins...eleven published libraries are embedded. These were kindly donated by six different research constellations...

..., D.C., we must be making a dent in the global coffee supply… My back-of-the-napkin calculations put the...attendees. And for those of you presenting, working, and networking at SfN, remember there are only 2 days ...community pulls off such an inspiring, thought-provoking, collaborative event – all while keeping the party...CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited to have Feng Zhang...we've spoken with are excited and passionate about making such tools open-access. Though it is time for me...

...knowledge, shaping their trainees’ very way of thinking too. Albert Einstein might have put it best when... (Brian, 1996). But is it possible to bring the kinds of transformative educational experiences afforded...happened in the lab, and he wanted to provide the same kinds of opportunities for his students. But he also found...research capabilities and laboratory and critical thinking skills (Knutson et al., 2010a,b). Classroom-based...student apprehensions about “failure” (protocols not working, hypotheses being rejected, etc.), while reminding...excellent training in improvisation and the latter making him a more effective motivator (“be like Tony Robbins...at other institutions. Provost believes these networking experiences increase student enthusiasm, and ...

...by (as the name suggests) cross-linking cellular proteins. Cross-linking is a chemical reaction that covalently...solutions.  Along with preserving your sample, cross-linking can block the epitope of some targets, preventing...antibody from binding (Fig.1). The level of cross-linking that occurs depends on the incubation time and ...may be able to recover good antibody binding by tweaking those parameters, but in some cases your antibody... good for epitopes that are sensitive to cross-linking, but it doesn’t preserve sample structure quite...decide which ones to use? Here are some tips for making those decisions: Always always always look at ...recovering epitope availability following cross-linking fixation; they require an additional step generally...