We narrowed to 432 results for: sequence

...edited sequence anneal with each other, while the 5’ flaps containing the original DNA sequence anneal...yields an edited sequence with the attB/P landing site. A recombinase integrates a sequence from a donor ...technique for precisely integrating large DNA sequences into a genome (Anzalone et al., 2022). While prime...recombinase handles the installation of the donor sequence. The latest work evolves and engineers Bxb1 recombinase...strategy. First, twin prime editing installs two sequences containing attB/P recombinase landing sites on...genome at this site, resulting in the integrated sequence between two attR/L sites. Image reused from Pandey...replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology,...

...quickly transfer a DNA sequence from the transposon vector to one of many TTAA sequences distributed throughout...the genome is 1 in every 256 base-pairs of DNA sequence, making it very useful for genetic engineering...transposon in a completely seamless manner, leaving no sequences or mutations behind. Furthermore, piggyBac™ offers...cells, without leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon...removed leaving behind no accessory transposon sequences.  The use of PBx is particularly important to ...donor removal, which alters the endogenous DNA sequence and has been suggested to cause cytotoxicity [...removal of the cassette later restores the target sequence. This allows researchers to avoid making unnecessary...

...). Like Cas9, dCas9 can bind to a specific DNA sequence via a targeting gRNA. But dCas9 does not cleave...One method required insertion of a recognition sequence into the genomic DNA that could then be recognized...purification of a specific genomic region and RNA sequencing (Fujita et al., 2015). Using similar affinity...gRNA-dCas9 complex. When combined with next-generation sequencing, this method allows for the recovery of a vast...telomeres using a combination of enChIP and RNA sequencing." PLoS One 10.4 (2015): e0123387. PubMed PMID...Toshitsugu, Miyuki Yuno, and Hodaka Fujii. "Efficient sequence‐specific isolation of DNA fragments and chromatin...Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression." Cell 152.5...

...an autonomously replicating sequence (ARS), a partial centromere sequence (CEN), both, or neither, depending... an autonomously replicating sequence (ARS) a partial centromere sequence (CEN) both or neither ...depending...molecular biologist has seen before, showing how DNA sequences are translated into amino acids. And I was astonished... Yeast shuttle vectors have these replication sequences for their yeast targets as well as a bacterial...incorporated into the host cell’s genome, so no special sequence is needed to retain the vector as cells divide...

...cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to create secondary structures...Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...think it’s easy to come up with a workable DNA sequence to encode your peptide of interest and produce... the possible codons at random and expect your sequence to express well in any organism. So what are the...1990). Controlling gene expression through gene sequence - Proteins that are encoded by codons with low...

...evolution techniques mimic natural selection of sequences that evolve a measurable property of a protein...of tobacco etch virus protease (TEV), a highly sequence-specific protease used to cleave affinity tags...encoding FPs tagged with the plasma membrane targetin sequence derived either from p63(1-29) and Lck. Image from...plasma membrane, they tested different target sequences for improved selectivity and found that the best... of MBP, followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide...nearly any gene via specific guide RNA (gRNA) sequences. But these approaches rely on continual expression...antiviral method to degrade RNA from SARS-CoV-2 sequences and live influenza A virus. It was demonstrated...

... signal sequence (gene start) and a transcription termination/polyadenylation signal sequence (gene end...viral sequences (e.g. promoters) can be studied by changing the minigenome cis-acting sequences and monitoring...approximately 19 kb in length. It contains short promoter sequences at its 3’ and 5’ ends that are crucial cis-acting... by the EBOV-specific gene start and gene end sequences. Thus, the minigenome contains all cis-acting ... under control of a T7 RNA polymerase promoter sequence. Thus, T7 RNA polymerase also needs to be present...

... efficient and precise tools for altering DNA sequences. These first base editors were both deaminases... things base editors really need from Cas9 are sequence specificity and the R-loop structure. In order...requires (1) a guide RNA, (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on... types, although efficacy varied among target sequences. The researchers hypothesized that the CGBE edit...different deaminase and UNG enzymes had different sequence preferences, which they defined thoroughly. Plus...outcomes of their various CGBEs at different target sequences and in different cell types, available at www....target loci (between 4.0% to 29.2% of studied sequences) (Huang et al., 2024). BE4max, a CBE, generated...

...target cell populations Enhancers are non-coding sequences of DNA that regulate gene expression by interacting... recognize and bind motifs within the enhancer sequence, then interact with mediator complexes and the...1). Although all cell types share the same DNA sequence, not all enhancers are functional in every cell...to different DNA motifs, results in a given DNA sequence serving as a functional enhancer in one cell type...tools for brain visualization Putative enhancer sequences for specific cell types can be identified based... potential enhancer candidates. To use these sequences for making functional genetic tools, enhancers...enhancer ID (h, m, or q, respectively). These sequences were then cloned into an AAV backbone containing...

...targeting power of CRISPR combined with the large sequence capacity of transposons open whole new worlds ...cofactors. The transposon proteins integrate a payload sequence into genomic DNA downstream from the Cas target... double-stranded breaks and can deliver large sequences and even entire genes. Figure 1: Mechanism... transposon proteins (Tns) to integrate large sequences into genomic DNA downstream of gRNA-specified ...site-specific recombinase to achieve insertion of long sequences. In their experiments, PASSIGE had higher efficiency...