... Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA Chapter 4... and Diplomacy, with a focus on international technology management, from the Fletcher School at Tufts...investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie Chen is a co-founder...and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software Engineer...

...current forming membrane pores) transfection. These techniques are still used and efficiently transfer plasmid...not been tested for HIV.  If not using proper technique, there’s always a small chance that a researcher...transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly after transfection...for virus titration: the p24 ELISA dosing method detects the expression of viral capsid antigen p24, the...Therapy, Dr. Ke Xu (Ed.), ISBN: 978-953-307-539-6, InTech. 4. Walter, W., and U. Stein. "Viral vectors for...

... itself was relatively easy. Figure 4: Detection of edited BRAF genomic loci in Cas9-expressing...dsDNA) at basepair mismatches. To use this tool to detect a BRAF gene edit, simply PCR-amplify the BRAF locus...be in the lab. Based on the power of available techniques and how quick and easy it is to share information...Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. 10.1038/nbt...Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed PMID...

... modular, standardized, disciplined, and open techniques. Chapter 4: Research projects underway in the...Synthetic Biology. Wide adoption of synthetic biology techniques in biology research as can already be seen with...University of Cambridge before spending time in biotech screening direct interactions between drugs and...investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising...

...research.     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform...high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or DNA. Now they have developed...developed this further to provide multiplexed detection, higher sensitivity, and more. Read the paper...and cleaves ssDNA which they have adapted for detecting SNPs. Learn more from this Addgene blog post...

...backgrounds! Show off your favorite reagent, scientific technique, or plasmid repository at your next meeting.   ...blog topic to learn more about FRET and other techniques using FPs! Otherwise, check out our plasmid and... browse plasmids by field, organism, research technique, and more. Download the green DNA background. ...those backgrounds representative of your favorite technique? Our lab-themed background is a great fit for ...

...depositors who quickly shared their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein...reagent sharing even broader and began working with tech transfer offices to make reagents available to scientists...extracting RNA without a kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas by Shravanti Suresh...University detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids deposited...

...classification system for the array of CRISPR technologies. Learning the general principles of this classification...systems. Type III systems are unique, as they can technically target both RNA and DNA, though it is largely...the first taught when introducing CRISPR as a technology. There are three subtypes, A–C, with the beloved...Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing. Cell ...Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system...

...this post even more useful to your colleagues. Technique: Probe Phage Genomes for Host Binding Proteins...editor’s choice posts this month. The first, "Technique: Probe Phage Genomes for Host Binding Proteins...from the University of Alberta and describes a technique you can use to find phage proteins that bind to...2) The post describes an ingenious but simple technique to screen for proteins that could be useful for...

...are swarming to see what can be done with this technology to help patients. Dr. Stoffels and others in ...months compared to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents..., the panelists agreed that, for example, the technology is already available today to cure sickle cell...really sophisticated”.     New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History...

...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ...question. Additionally, a concurrent Nature Biotechnology article describes the development of a mouse...accessible before are now accessible using this technology.” GeCKO library for genomic screens Using lentivirus... Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs...

...sensitive detection of the plant hormones strigolactones By Fabienne Furt Current methods used to detect strigolactones...CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New ready-to-...intensity, it provides a direct and quantitative detection of SLs. The authors also showed that the receptor-based...

...purification tools (and can have secondary functions as detection aids.) When a single protein is expressed, with...from solution. MBP antibodies are available for detection and/or purification of MBP-tagged proteins. The...alternative to amylose resin and also provides a visual detection mechanism via applications such as western blot...of interest, especially if downstream antibody detection is desired… and yes, we have an antibody for that...

...material for a ChIP-seq experiment can be a major technical hurdle.   ChIP-seq requires  cross-linking during...improvement and simplification of the original technique, specifically in terms of initial steps of cell...ChIP-seq, especially in situations when it is technically challenging to collect large numbers of cells...the CUT&RUN method, it seems likely that this technique will quickly become a standard method alongside...

...Snapgene generated maps provide improved feature detection with an easy to use interface. We regularly use... linear or circular sequence. Next, we use the “Detect Common Features” function to find and annotate ...s “Import primers from a list” function, we can detect all of the primers we have designed that will anneal...plasmid maps and sequences use the same feature detection software as the desktop version of Snapgene. When...

...limitations by using gas vesicle reporters that are detectable by an inexpensive and widely available imaging...fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail to pinpoint...each ARG’s distinct ultrasound fingerprint can be detected. Bourdeau et al calls this method acoustic collapse... in relation to internal organs. The limit of detection for arg1 expressing ECN is 109 cells/ml, which...

... be your critical experiments and why? What techniques and skills do you need to use to reach these ...address a topic in a mechanistic way using the technology available.” Choosing a research project “entails...entails good judgement with the current technology to identify a problem, asking what approach will I take... importance of combining “new discoveries or technologies increase chances that your project will be fresh...

...context of IHC, but the problem can impact other techniques like Western blots or immunocytochemistry (ICC... described here may be as effective for other techniques, but it is important to be aware that the issue...Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free Reagents, Immunofluorescence..., Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding of human...

...AAV viral preps to easily find and share useful technical data. These data focus on experimental design ... These questions are focused on injection and detection methods. For example, you can provide location...you wait until performing the experiment? What detection methods did you use for expression of AAV cargo...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources on...

...no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs...called TOPO-TA) cloning; however, the TOPO cloning technique has also be adapted for blunt end cloning. So ... DNA ends to facilitate replication. The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase...Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...