We narrowed to 697 results for: Tec

... Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on Addgene.org... Hot Plasmid Pages to See the Latest Plasmid Technologies ...

...assay, sequencing, RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites...design, cloning, and delivery, as well as their techniques for creating deletions, identifying these deletions...

...They generated these editors using the BE4max architecture. The new editors reported in this study have...relative to BE4max.  Read the paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter...

...Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine. Neuron, 102(4), 745-761.e8....Fluorescent Biosensors Which Fluorescent Microscopy Technique is Best for Me?  In Living Color: The Skinny on...

... curator of the thousands of amazing plasmid technologies contributed by our depositors, Addgene strives...certainly not least, we often feature new plasmid technologies on our blog and encourage you to fill out this...

...Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression of an unknown...plasmid sharing? Check out our Top 10 Plasmid Technologies blog post Or look at our "10 Years of Plasmid...

...theory or practice; business-like or artistic; tech, anti-tech or pragmatic.” Advice for pursuing careers...

...reported doing in a paper that appeared in Nature Biotechnology back in 2011 and then in another Nature Methods...protein for in vivo imaging. Filonov et al (Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.) Near-infrared...

...intertwined. In this blog we will review how CRISPR technology has brought drug and genetic screens together...of control have also been added to the CRISPR technology to allow time-controlled edits to occur, such...

...graduate student down the hall who got it from some technician downstairs who got it from who knows where), ...Cellosaurus, a Cell-Line Knowledge Resource. J Biomol Tech. 2018; 29(2): 25-38....

...Chemogentic and optogenetic technologies have pushed the boundaries in neuroscience by granting targeted...activity. While they serve similar purposes, both techniques offer researchers different advantages and limitations...

...overcomes all of these labor-intensive and invasive techniques to reliably label any post-mitotic cells in Drosophila...et al., 2019. Applications for SPARC SPARC technology is versatile and can be applied across cell types...

...also do videos demonstrating an experiment or a technique to students, like how to pour a gel [for] electrophoresis...really cool to take a step back and look at new technologies in education and ask ‘Can we implement that?...

...interview three science communicators who work in the biotech, education, or nonprofit industries. First up ... a job as a Science Writer at Integrated DNA Technologies (IDT). “It was fairly accidental. I was finishing...

...services we provide like quality control, curation, technical service and open-access educational resources....nonprofit? One big reason is that scientists and technology transfer offices trust us to work for them with...

...sections. The focus groups allowed us to try out techniques that were discussed in the larger sessions and... Well what does that mean? If you start a new technique, then type up the materials and methods. Over ...

...learn about Synthetic Biology and exciting new technologies in the field. One of the main aims of the society...networking to promote the creation of start ups and biotech spin offs. The Synthetic Biology Society also aims...

...field. Advances in genome engineering & CRISPR technology That afternoon's workshop, entitled "Advances...amazement at how much has been done with CRISPR technology in just 2 years and excitement about how much...

...The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833...

...secondary to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect Protein B...

...STAR (Situation, Task, Action, Result) interview technique, which has been addressed in much better pieces...story should be too. Use drama Use any rhetorical technique you have in your arsenal to make sure people pay...

...bacterial strains, such as Stbl3s. ITR loss can be detected by restriction digest using enzymes, like SmaI...Kamens J, Fan M (2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...

...containing lentiviral vectors the iBARs can be detected by next generation sequencing.  In order to avoid...measurements, or monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin...

...reliably confirm the identity of AAV genomes and detect contaminating AAV DNA. In addition, we wrote an...minutes and the scripts  can be easily customized to detect other sequences of interest.   While the VGS process...

...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative, will allow Addgene to... The development of monoclonal antibody (mAb) technology circumvented many of these issues by creating...

...Their aim - to red-shift the boundaries of FP technology by shifting the emission maxima toward the red...However, a challenge in optimizing base editing technologies across several cell types is the lack of a rapid...

... genetically encoded antibody based probe that detects the widely used HA epitope in diverse cellular ...dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and scaffolds...

...postdoc association, started a speaker series or biotech club, managed their own blog or podcast, initiated...the board of a professional society, solved a technical problem, had an alter ego as a mad scientist for...

...scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that ...applied for the government sponsored Paycheck Protection Program loan and received enough funds to make...

...desired identity. The development of single cell technologies, such as single cell RNA-seq (scRNA-seq), now...multi-generational cell lineage trees. CellTags were detected over a 10-week period. Their expression decreased...

... to detect mutants, but this method limits screening to a few hundred worms, making the detection of rare...

...especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...capabilities, courses and office skills. I removed my techniques and protocols, courses taken during my PhD and...

...translational approaches for scientific tool and technique development. However, careers outside of the lab...Management Leadership Networking University technology transfer office positions   Market analyses...

...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9... for microscopy by: Susanna Stroik Microscopy techniques have been moving at the speed of light over the...

...viral particles per cell, you may not be able to detect AAV-mediated gene expression in a target cell. ...confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production...

...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020...

...conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...you stay connected. If possible, test out the technology before the conference begins.“There were some...

... eat, and rest. Try the Pomodoro Technique or the Flowtime Technique. Co-work virtually. With co-working...

...particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI.   Additional...resources Find protocol videos to teach basic lab techniques Plasmid Land: The plasmid cloning game ...

...measurements such as patch clamp recordings. However, technical difficulties such as small fluorescence changes...QuasAr-mOrange and MacQ-mCitrine. All of these sensors can detect action potentials in worms with fluorescence changes...