We narrowed to 803 results for: Cre

...expression transposases leading to increased integration frequency. (C) Increasing rounds of transfer on transposition-inducing...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

...each project. This creates accountability on the part of each developer and creates trust amongst the ... Addgene grows and evolves, we will be here to increase all of our teams’ efficiency and productivity....

...prognoses. Survival rates for mesothelioma are creeping upwards, and while most people still die within...world. New mesothelioma cases could be greatly decreased through a ban on asbestos. Nonprofits like the...Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional Resources on Addgene.org ...

...glutathione reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

...you need to speak to start creating slides or thinking about content. Create a Google.slides or Powerpoint...capture interesting key results, striking images, creative ideas, and engaging stories as you encounter or...

...percentage of dystrophin-expressing muscle cells increases over time, and all three groups confirm dystrophin...muscle stem cells are modified by this approach, increasing the possibility that edits will persist long-...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the ...

...should be able to apply someone else's new and incredibly useful technique simply by following the abbreviated...49:45 - 1:18:45), there are also initiatives to create more reliable reagents for future research. I’ve...IPI is working to address this issue directly by creating well characterised antibodies against all human...

...fluorescent dyes, and fluorescent ligands. Figure created with BioRender.com. Fluorescence refers to...fluorescent protein tags, such as high specificity, increased versatility in probe attachment, and compatibility...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction with receptors...

...of antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If...interest. By correlating your data sets, you can increase confidence that the labeling observed represents...experiment. We find it useful to start with a screening procedure in easily genetically modified cell ...

...is a significant hurdle made worse by an ever increasing marketplace. Since 2013 about 11 tools have been...the user is expected to interact with the tool (a screen shot and overview of the table can be found above...re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile...

...for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR base editors...Segment  Hot Plasmid 2: Ouabain selection for increased CRISPR editing efficiency. Listen to the Segement...

...various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR...

...platform changes, such as limiting access and increased priority given to paid content regardless of user...preserve the knowledge, conversations, and community created and shared on our feed, we will not delete @addgene...

...celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured in our blog ...Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene writers High Complexity...

...and Instagram and we’re excited to share these creative entries as the winners of the 2019 contest. ...material tree We often get submissions of decorations created from lab materials. This year, one of the winners...

... guide that curates this information. And so I created the Codon Guide to Synthetic Biology in the hopes...open-access resources whenever possible.  Image credit: Niko McCarty; adapted from Bruce Wetzel and Harry...

...at the University of Amsterdam. In addition to creating and sharing many great fluorescent protein tools...probes and more. Chapter 5 - mTurquoise II and the creation of improved fluorescent proteins through mutagenesis...

...blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for use in mice and human...from 2022! Wow! Our depositors do some really incredible science - I hope you've enjoyed learning about...

...Microbiology Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed...Addgene has so many of these resources that we created filters just for them on our search page. Use the...

...can stage in a lab diorama. Or maybe you want to create a stack of wrapped gifts using life science catalog...Twitter and Instagram shortly after. Have fun, be creative, and enjoy the end of year festivities - however...

...materials into holiday decorations. We appreciated the creative uses of old posters, pipette tip boxes, and more...working on our reports/lab stuff for a few minutes to create this lovely winter scene out of stuff we found ...

...fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments. Professor Fessenden's... also really helped put this technology into a concrete context. I recommend this post to anyone wondering...

...incorporate to reduce your lab consumption and increase your lab sustainability. For my other posts on...Subscribe to the Addgene blog Learn about other creative approaches for lab sustainability Resources on...

...Data reports  Each antibody submission is used to create a data report, which contains experimental data...the experimental conditions.     Figure 4: A screenshot of the materials and methods section for the ...

...complex via the fused HUH endonuclease PCV. This increases HDR efficiency up to 30-fold. Read the paper ...variants with mutations in the rat APOBEC1 that decreases the number of off-target RNA edits by >390 fold...

... doesn’t. Reports receive a DOI for citation, crediting researchers when their data is used by the community...understand our role in opening up science to help create new knowledge and research opportunities. I have...

... affiliated with the recipient organization can create an Addgene account and place an order: a purchasing...order to generate a PO, learn more about how to create a quote. How do I pay the invoice? After your ...

...and energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags) less likely...inntags are indeed innocuous, Georgieva et al. created tagged versions of the cyclin Cdc28 and the chaperone...

...without exclusivity. Chapter 11: The Stem Cell Bank (CReM Bank) at the Center for Regenerative Medicine and... a member of the Addgene board of directors - increasing open source sharing and helping us stick to our...

...compensation committee. The compensation committee creates a compensation philosophy that helps guide employee...rescue organization where I foster dogs and help screen dog adoption applications. My most recent foster...

...into our lab lives. Read on to learn about some creative approaches to lab sustainability, and find ways...learn that it hasn’t even been 50 years since the creation of the United States Environmental Protection ...

... describes an ingenious but simple technique to screen for proteins that could be useful for a wide variety...event gives me the opportunity to dress up as Mr. Incredible, an added bonus. You can find the rest of our...

...can almost be boiled down to one, key concept: Creating a culture of excellent, effective communication...practice and training. This is important because it creates a culture of engagement in the issues at hand and...

...happen anywhere!). The pair quickly teamed up to create Phage Directory in a whirlwind three days in November...purified before sending the phages to the clinicians. Creating a repository of phage data and information Since...

...for research by anyone. As infection rates were increasing in the United States, and many universities and... community speed research is to offer plasmids created as part of research projects, another way is to...

... are we! This expansion will give researchers increased flexibility when planning their research and expanded...This split allows us to provide researchers with increased choice and flexibility; while ensuring we can ...

... to GRABNE, and having both colors allows for increased options for multiplexing in experiments, including...both nLightG and nLightR showing significantly increased selectivity for norepinephrine over dopamine. ...

...ahold of your destinies” and also suggested some concrete actions that can be taken now to solve some of...the possible science careers. She advocated for increasing the value and priority put on good mentoring ...

...proposition, explained Michelle Baird, who has created plasmids as a technician in the Davidson lab for...prompted him to deposit the tools he and his team created with the research community at large, "[Fluorescent...

...submicromolar) DA affinity and high-magnitude responses (increases in fluorescence) at low concentrations (e.g., ...The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: ...

...operons from two different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli... and have already developed a high-throughput screening method for serial acoustic collapse to aid in ...

...DNA-protein interactions, the reliance on fixation does increase the chance of false-positives, as proteins may...RUN doesn’t require fixation and significantly decreases background noise, reducing the read depth necessary...

...critical thinking and problem solving abilities, increased my technical know-how, and helped develop my science...scientific knowledge and technical know-how that increased my autonomy and satisfaction with my work.  5....

...Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA, for example...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the...

... at eScience Labs, an educational company that creates hands-on science lab kits and digital curricula...alternative science careers here Interested in creative science education tools? Check out this Addgene...

...accurately reflect our culture and outlook: Addgene increases productivity by providing world-wide access to...leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters a culture...

...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  ... mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al., 2021....

... to help you do better research. CRISPR is an incredibly fast-moving field, and we want to make it easy...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

...leader’s job to provide the driving inspiration, to create a productive culture and to correct systemic problems...try operating in a different role?  I think the secret sauce can be summed up in one word – initiative...

... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...your applications and experimental design. You can create a new strain with a disrupted gene by transforming...