We narrowed to 645 results for: Rest

...has made it possible to eliminate geographical restrictions in life science research." Claim Your Award ...

...content that scientists will find useful and interesting than that will simply get people ordering plasmids...

...is possible in your eyes so their confidence is restored. A good manager does not hold grudges. Never...

... reagents and money.  This was the start of my interest in “stingy science”.  Practicing thrift and conservation...

...separate resistance cassettes and your gene of interest is cloned into/inactivates or completely removes...

...about workplace bullying featured on the always interesting Tenure, She Wrote blog. Unfortunately, academic...

...viral anti-CRISPR genes. These data highlight an interesting "arms race" between phages and prokaryotes; the...

...directed repair (HDR) are echoed in the amount of interest in base editors, which alter genomic DNA without...

... my job I most enjoy is building and telling interesting stories — whether I’m working with a talented...

...mitophagy. Rosella can also be fused to a protein of interest to track the autophagy of that protein. Find ...

...required three components: a master mix, the DNA of interest, and cell extract.    Graphical Abstract...

...use two antibodies - one against your target of interest and a second against the first antibody. When ...

...would be wireless, able to monitor outcomes of interest, and stimulate or manipulate responses through...

...the lookout for aggregation of your protein of interest!   Ready to start epitope tagging?   References...

... also need to be transfected into the cells of interest. In contrast, RNA guides must be expressed from...

...strains you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening Getting...

...Komuhendo is a Lab Technician at Addgene who is interested in medicine and music.       Additional Resources...

...realized that it was easy to find people who were interested. For example, our journal club discussion on ...

...and if you have any tips, input, or stories of interest about library use, let us know in the comments...

... is opened and equilibrium with ambient air is restored. We have no evidence to suggest that the transient...

...vehicle for drug delivery that has recently gained interest special contexts, such as the delivery of therapeutic...

...editing, in virtually any cell type or organisms of interest with only minimal genetic payloads. Different ...

...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...

...drawing me towards the Broad. In order to ask interesting biological questions, you need a tremendous amount...

...browsing job postings and look at the jobs you are interested in. Consider listing the skills you already have...

...assayed conveniently. A direct test for your gene of interest may be another method to assess transfection success...

...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern...

...and photobleaching. Researchers have long been interested in using luminescence to get around these issues...

...Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ...

...multi-gene editing with a single crRNA array. Are you interested in using Cpf1 multiplexing in your research? ...

... developments on this project and on all the interesting things going on at Addgene. Sharing speeds science...

...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...

...ability to reversibly bind and release a protein of interest in response to light allows for tagless protein...

... ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology...

...gRNA and enrich cells for your CRISPR mutant of interest, give these methods a try.  References Højland...

...These modifications result in conformationally restricted RNA which improves mismatch discrimination and...

...understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should aim to keep...

...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...

...protein in an experiment with many proteins of interest! Research the brightness of your fluorophores ...

...primary antibody but doesn’t bind your antigen of interest. Isotype controls are used to make sure the staining...

...thousands of reporter constructs with a gene of interest already inserted. In addition, we have recently...

... binds to a specific region of your protein of interest. For example, if you are studying a protein that...

...University of Pennsylvania. She is particularly interested in science communication and epigenetics. Follow...

...multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis...

...share it as soon as possible. Addgene: How much interest are you seeing in this new tool? Mendenhall: We...

...great potential for multiplexed genome editing. Interested in using PTG plasmids in your research? Check...

...off, to reversibly activate or repress genes of interest. They also “showed that different guide RNAs have...

...biotech startup process and work environment! Interested in Synthetic Biology? Read On! Synthetic Biology...

...whether your manuscript fits the scope and the interest of the journal. Be sure to check the Author Guidelines...

...there a senior person whose career path you are interested in following? Someone you admire for their insight...