We narrowed to 528 results for: Set

...amounts of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein...

...enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest explorations...

...As we pointed out last year2, delivering a DNA cassette encoding the cas9 gene and single guide RNAs (...

... Seth Kasowitz...

... vast amount of data from a single experimental setup. As we’ve seen for many other CRISPR techniques,...

...12727116. Borkenhagen, L. K., Fieldhouse, J. K., Seto, D., & Gray, G. C. (2019). Are adenoviruses zoonotic...

...to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology potential...

...useful in gene therapy applications where a distinct subset of the cellular population needs to be targeted...

...are highly repetitive, such as those containing cassettes of the same gene multiple times.  *As of March...

...This strain is then transformed with the sgRNA cassette on a plasmid expressing mCherry and resistance...

...fails to upregulate transduction efficiency in the marmoset brain." Neuroscience letters 665 (2018): 182-188...

...Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ...

...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

...the technical implementation of your optogenetics setup (viral expression, efficiency of laser light transmission...

...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic...

...cases, you should validate it for your particular setup. We recommend starting by searching the literature...

...insertions such as fluorescent proteins or selection cassettes. This class of template should have homology arms...

...prokaryotic lineages revealed a curious anomaly amongst a subset of methanogenic archea: these organisms lacked ...

...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation...

...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells...

...training in the academic research environment is setup to prepare trainees for further work in academia...

...Figure 1: Example of an experimental optogenetic setup. A light source delivers light pulses to the subject...

...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience...

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...emission depletion (STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with standard confocal microscopy...

...Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node." Proceedings of...

...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

... pan-mutation editing Benjamin Kleinstiver (Massachusetts General Hospital / Harvard Medical School) presented...